Gene expression

•Download as PPTX, PDF•

1 like•53 views

Venkata Satish Kola

journal discussion

Health & Medicine

PRESENTER:DR.K.V.SATISH
MODERATOR:DR.K.V.V.S.SATYA KUMAR SIR
MD(HOM)
HOD,PG DEPARTMENT OF REPERTORY
DATE:10/10/2018

 GENE is a sequence
of DNA or RNA that codes for a
functonal molecule

 In genetics, gene expression is the most
fundamental level at which the genotype gives
rise to the phenotype, i.e. observable trait.
The genetic code stored in DNA is
"interpreted" by gene expression, and the
properties of the expression give rise to the
organism's phenotype. Such phenotypes are
often expressed by the synthesis of proteins
that control the organism's shape, or that act
as enzymes catalysing specific metabolic
pathways characterising the
organism. Regulation of gene expression is
thus critical to an organism's development

 Transcription - Transcription is the
process by which a segment of DNA is
used to generate an RNA template. The
DNA segment is “read” by an enzyme
called RNA polymerase, which produces
a strand of RNA that is complimentary to
the DNA.

 Processing - This primary RNA
transcript is then modified to convert it
into mature messenger RNA (mRNA)
that can be used in translation.

 RNA export - The majority of mature
RNA is then transported from the
nucleus to the cytoplasm. Although
some RNAs function in the nucleus,
most are carried through pores in the
nucleus into the cytosol, including all
RNAs involved in protein synthesis.

 Translation - The final mRNA carries the
information needed to code for proteins. Every
three base pairs on the mRNA corresponds to
a binding site for a transfer RNA (tRNA) which
carries an amino acid. The amino acids are
then linked together in a chain by a ribosome
to create a rudimentary protein chain.
 Protein folding - The long chain of amino
acids folds to form a three-dimensional
structures using enzymes called chaperones.
This three-dimensional structure is the final,
functional form of the protein.

“Life Time Achievement Award”by
European Committee of Homeopathy,

 Homoeopathic remedies carry a specific
signals that can be identified by specific
receptors and can act as a trigger to turn on
and off some relevant genes ,initiating the
cascade of gene actions to alter and correct
the gene expressions that had gone wrong to
produce the disorder/disease condition

What's hot

Protein synthesis

JohnCabrido

Ribosome in relation to cell growths & vision

rajendra prasad central agricultural university

Ribosomes: Unravelling the structure of Protein Factories

Sai Ram

Protein synthesis

Jigar Patel

Translation in Prokaryotes

Sonia John

AP Bio Ch 10 Power Point

Anthony DePhillips

Pathways of intracellular signal transduction

MohamedEramHosen

Ribosomes- Protein Synthesis

Hassan Ahmed Khan

ribosomes

Dilip Pandya

Protein synthesis (translation)

Sihabudin Sihab

Unit 9 Dna Translation And Mutation

Olympus High School - Jeff Taylor

Regulation of gene expression

MANSI

transciption powerpoint

Nikka Bañez

protein synthesis

Nawfal Aldujaily

Ribosomes - Origin, Chemical composition, Structure and Function

Kalpesh Damor

Ribosome ppt (introduction structure & Function)

Dryogeshcsv

Gene expression concept and analysis

Noha Lotfy Ibrahim

5’ capping

EmaSushan

Regulation of gene expression (molecular biology)

IndrajaDoradla

Prokaryotic transcription

Prasanna R Kovath

What's hot (20)

Protein synthesis

Ribosome in relation to cell growths & vision

Ribosomes: Unravelling the structure of Protein Factories

Protein synthesis

Translation in Prokaryotes

AP Bio Ch 10 Power Point

Pathways of intracellular signal transduction

Ribosomes- Protein Synthesis

ribosomes

Protein synthesis (translation)

Unit 9 Dna Translation And Mutation

Regulation of gene expression

transciption powerpoint

protein synthesis

Ribosomes - Origin, Chemical composition, Structure and Function

Ribosome ppt (introduction structure & Function)

Gene expression concept and analysis

5’ capping

Regulation of gene expression (molecular biology)

Prokaryotic transcription

Similar to Gene expression

Gene expression & regulation

Dr.SIBI P ITTIYAVIRAH

Bruno Mmassy

Gene expression system

Dilip22Morani

Gene expression

Dr. Muhammad Atif Raza

Transcription in prokaryotes and eukaryotes.pdf

ssuser880f82

POST TRANSCRIPTIONAL MODOFICATION.pptx

SuganyaPaulraj

Genes in Action

Martin Jellinek

Provide an in depth description of biological information transfer (what is the chemistry underlying each information transfer event, which nucleotide sequences are involved etc.) Solution The genetic information is stored in Deoxyribonucleic acid,DNA. DNA contains the information needed to build an individual. Genetic information is transferred from DNA and converted to protein.RNA molecules work as messengers.Proteins are the biological workers.Information of the DNA is copied to a RNA molecule in transcription.RNA directs the protein synthesis in a translation.Protein’s 3D structure determines it’s function.Information transfer only in one direction. The biological information flows from DNA to RNA,and from there to proteins.It is ultimately the DNA that controls every function of the cell through protein synthesis.As a carrier of genetic information,DNA in a cell must be duplicated (replicated),maintained and passed dawn accurately to the daughter cells. DNA is deoxyribonucleic acid,which is found in chromosomes, contains inherited information,they are made up of nucleotides,and are what make up genes. A nucleotide is composed of a sugar (deoxyribose),a phosphate group,and a base.There are 4 bases found in DNA, Adenine (A),Thymine (T),Guanine (G),and Cytosine (C).Adenine and guanine are double ring bases while thymine and cytosine are single ring bases.Nucleotides are joined to each other by covalent bonds between the phosphate group of one nucleotide and the 3\' carbon atom of the deoxyribose (sugar) of the next nucleotide.Each DNA molecule is unique because the order of nucleotides is unique. The order of nucleotides determines the order of amino acids in a protein.RNA is a nucleic acid composed of nucleotides and consists of one strand of nucleotides.There are three different types of RNA- Ribosomal,Messenger,and Transfer.Ribosomal RNA is the RNA molecules found in ribosomes. The large subunit RNA contains the enzymatic activity that makes the peptide bonds between amino acids. Messenger RNA is what controls the order of amino acids in a protein and determines which gene it codes for.Transfer RNA brings amino acids to ribosomes.The transfer RNA has two recognition sites- one recognizes an amino acid and the other recognizes one codon.The transfer RNA brings the the correct amino acid to the ribosome. Transcription is the process by which the information contained in a section of DNA is replicated in the form of a newly assembled piece of messenger RNA (mRNA).Enzymes facilitating the process include RNA polymerase and transcription factors.In eukaryotic cells the primary transcript is pre-mRNA. Pre-mRNA must be processed for translation to proceed.Processing includes the addition of a 5\' cap and a poly-A tail to the pre-mRNA chain,followed by splicing.Alternative splicing occurs when appropriate, increasing the diversity of the proteins that any single mRNA can produce.The product of the entire transcription process is a mature mRNA ch.

Provide an in depth description of biological information transfer (.pdf

MALASADHNANI

Gene expression: Translation and Transcription

Cyra Mae Soreda

Prokaryotic vs eukaryotic 3

GGS Medical College/Baba Farid Univ.of Health Sciences.

The molecular genetics of gene expression by : CHRISTONE JAY NOVIDA

Cj Novida

Gene expression&regulation part ii

Dr.SIBI P ITTIYAVIRAH

• Define transcription • Define translation • What are the 3 steps of translation? • Define the “genetic dogma” • What is the function of Transfer RNA? • What is the function of RNA polymerase? • What is the function of DNA polymerase? • Define “splicing of RNA” • What is an exon? • What component of the cell does the translation? • What molecule in the cell does transcription? • What are the functions of: operon, promotor? • What is the difference between inducible operon and repressible operon? Solution • Define transcription Transcription is the process of making an RNA copy of a gene sequence. This copy, called a messenger RNA (mRNA) molecule, leaves the cell nucleus and enters the cytoplasm, where it directs the synthesis of the protein, which it encodes. Here is a more complete definition of transcription. • Define translation Translation is the process of translating the sequence of a messenger RNA (mRNA) molecule to a sequence of amino acids during protein synthesis. The genetic code describes the relationship between the sequence of base pairs in a gene and the corresponding amino acid sequence that it encodes. In the cell cytoplasm, the ribosome reads the sequence of the mRNA in groups of three bases to assemble the protein. Here is a more complete definition of translation: • What are the 3 steps of translation? Step # 1. Initiation: Initiation of translation in E .coli involves the small ribosome subunit, a mRNA molecule, a specific charge initiator tRNA, GTP, Mg++ and number of proteinaceous initiation factors (IFs). These are initially part of the small subunit and are required to enhance binding affinity of the various translational components (Table 8.1). Unlike ribosomal proteins, IFs are released from the ribosome once initiation is completed. Step # 2. Elongation: Once both subunits of the ribosome are assembled with the mRNA, binding site for two charged tRNA molecules are formed. These are designated as the ‘P’ or peptidyl and the ‘A’ or aminoacyl sites. The charged initiator tRNA binds to the P site, provided that the AUG triplet of mRNA is in the corresponding position of the small subunit. The increase of the growing polypeptide chain by one amino acid is called elongation. Step # 3. Termination: Termination of protein synthesis is carried out by triplet codes (UAG, UAA, UGA; stop codons) present at site A. These codons do not specify an amino acid, nor do they call for a tRNA in the A site. These codons are called stop codons, termination codons or nonsense codons. The finished polypeptide is still attached to the terminal tRNA at the P site, and the A site is empty. • Define the “genetic dogma” A theory in genetics and molecular biology subject to several exceptions that genetic information is coded in self-replicating DNA and undergoes unidirectional transfer to messenger RNAs in transcription which act as templates for protein synthesis in translation • What is the function of Transfer RNA? The tRNA molecule, or tr.

• Define transcription• Define translation• What are the 3 steps.pdf

arihantelehyb

Genetics

Dr. Darshanaa Arunachalam

Control of gene expression ppt

Ibad khan

Chapter 20 Molecular Genetics Lesson 2 - Genes_Transcription and Translation

j3di79

Transcription

sadhana gupta

Gene expression Gene expression is the process by which the genetic code - the nucleotide sequence - of a gene is used to direct protein synthesis and produce the structures of the cell. Genes that code for amino acid sequences are known as \'structural genes\'. The process of gene expression involves two main stages: Transcription: the production of messenger RNA (mRNA) by the enzyme RNA polymerase, and the processing of the resulting mRNA molecule. Translation: the use of mRNA to direct protein synthesis, and the subsequent post-translational processing of the protein molecule. Some genes are responsible for the production of other forms of RNA that play a role in translation, including transfer RNA (tRNA) and ribosomal RNA (rRNA). A structural gene involves a number of different components: · Exons. Exons code for amino acids and collectively determine the amino acid sequence of the protein product. It is these portions of the gene that are represented in final mature mRNA molecule. · Introns. Introns are portions of the gene that do not code for amino acids, and are removed (spliced) from the mRNA molecule before translation. Gene control regions · Start site. A start site for transcription. · A promoter. A region a few hundred nucleotides \'upstream\' of the gene (toward the 5\' end). It is not transcribed into mRNA, but plays a role in controlling the transcription of the gene. Transcription factors bind to specific nucleotide sequences in the promoter region and assist in the binding of RNA polymerases. · Enhancers. Some transcription factors (called activators) bind to regions called \'enhancers\' that increase the rate of transcription. These sites may be thousands of nucleotides from the coding sequences or within an intron. Some enhancers are conditional and only work in the presence of other factors as well as transcription factors. · Silencers. Some transcription factors (called repressors) bind to regions called \'silencers\' that depress the rate of transcription. Transcription Transcription involves four steps: Transcription is the process of RNA synthesis, controlled by the interaction of promoters and enhancers. Several different types of RNA are produced, including messenger RNA(mRNA), which specifies the sequence of amino acids in the protein product, plus transfer RNA (tRNA) and ribosomal RNA (rRNA), which play a role in the translation process. 1. Initiation. The DNA molecule unwinds and separates to form a small open complex. RNA polymerase binds to the promoter of the template strand. 2. Elongation. RNA polymerase moves along the template strand, synthesising an mRNA molecule. In prokaryotes RNA polymerase is a holoenzyme consisting of a number of subunits, including a sigma factor (transcription factor) that recognises the promoter. In eukaryotes there are three RNA polymerases: I, II and III. The process includes a proofreading mechanism. 3. Termination. In prokaryotes there are two ways in which transcription is ter.

Gene expressionGene expression is the process by which the genetic.pdf

ANSAPPARELS

Chapter 8: Microbial Genetics * Plasmids Exist in Cells Separate from Chromosomes Big Picture: Genetics The science of heredity Central dogma of molecular biology Mutations Gene expression controlled by operons Alteration of bacterial genes and/or gene expression Cause of disease Prevent disease treatment Manipulated for human benefit Big Picture: Genetics Structure and Function of the Genetic Material Learning Objectives 8-1 Define genetics, genome, chromosome, gene, genetic code, genotype, phenotype, and genomics. 8-2 Describe how DNA serves as genetic information. 8-3 Describe the process of DNA replication. 8-4 Describe protein synthesis, including transcription, RNA processing, and translation. 8-5 Compare protein synthesis in prokaryotes and eukaryotes. Structure and Function of the Genetic Material Genetics: the study of genes, how they carry information, how information is expressed, and how genes are replicated Chromosomes: structures containing DNA that physically carry hereditary information; the chromosomes contain genes Genes: segments of DNA that encode functional products, usually proteins Genome: all the genetic information in a cell Structure and Function of the Genetic Material The genetic code is a set of rules that determines how a nucleotide sequence is converted to an amino acid sequence of a proteinCentral dogma: Genotype and Phenotype Genotype: the genetic makeup of an organism Phenotype: expression of the genes DNA and Chromosomes Bacteria usually have a single circular chromosome made of DNA and associated proteins Short tandem repeats (STRs): repeating sequences of noncoding DNA Figure 8.1 A Prokaryotic Chromosome Chromosome The Flow of Genetic Information Vertical gene transfer: flow of genetic information from one generation to the next Horizontal gene transfer: flow of genetic information between individuals of the SAME generation (see the middle portion of the next slide!) Figure 8.2 The Flow of Genetic Information Parent cell DNA Genetic information is used within a cell to produce the proteins needed for the cell to function. Genetic information can be transferred horizontally between cells of the same generation. Genetic information can be transferred vertically to the next generation of cells. New combinations of genes Translation Cell metabolizes and grows Recombinant cell Offspring cells Transcription DNA Replication DNA forms a double helix “Backbone” consists of deoxyribose-phosphate Two strands of nucleotides are held together by hydrogen bonds between A-T and C-G Strands are antiparallel Order of the nitrogen-containing bases forms the genetic instructions of the organism DNA Replication One strand serves as a template for the production of a second strand Topoisomerase and gyrase relax the strands Helicase separates the strands A replication fork is created DNA Replication DNA poly ...

Chapter 8 Microbial GeneticsPlasm

JinElias52

please explain transcription and translation Solution Ans: Transcription is the process of making an RNA copy of a gene sequence. This copy, called a messenger RNA (mRNA) molecule, leaves the cell nucleus and enters the cytoplasm, where it directs the synthesis of the protein, which it encodes. Translation is the process of translating the sequence of a messenger RNA (mRNA) molecule to a sequence of amino acids during protein synthesis. The genetic code describes the relationship between the sequence of base pairs in a gene and the corresponding amino acid sequence that it encodes. In the cell cytoplasm, the ribosome reads the sequence of the mRNA in groups of three bases to assemble the protein. Transcription is the process by which DNA is copied (transcribed) to mRNA, which carries the information needed for protein synthesis. Transcription takes place in two broad steps. First, pre- messenger RNA is formed, with the involvement of RNA polymerase enzymes. The process relies on Watson-Crick base pairing, and the resultant single strand of RNA is the reverse- complement of the original DNA sequence. The pre-messenger RNA is then \"edited\" to produce the desired mRNA molecule in a process called RNA splicing. Formation of pre-messenger RNA The mechanism of transcription has parallels in that of DNA replication. As with DNA replication, partial unwinding of the double helix must occur before transcription can take place, and it is the RNA polymerase enzymes that catalyze this process. Unlike DNA replication, in which both strands are copied, only one strand is transcribed. The strand that contains the gene is called the sense strand, while the complementary strand is the antisense strand. The mRNA produced in transcription is a copy of the sense strand, but it is the antisense strand that is transcribed. Ribonucleotide triphosphates (NTPs) align along the antisense DNA strand, with Watson-Crick base pairing (A pairs with U). RNA polymerase joins the ribonucleotides together to form a pre- messenger RNA molecule that is complementary to a region of the antisense DNA strand. Transcription ends when the RNA polymerase enzyme reaches a triplet of bases that is read as a \"stop\" signal. The DNA molecule re-winds to re-form the double helix. RNA splicing The pre-messenger RNA thus formed contains introns which are not required for protein synthesis. The pre-messenger RNA is chopped up to remove the introns and create messenger RNA (mRNA) in a process called RNA splicing Alternative splicing In alternative splicing, individual exons are either spliced or included, giving rise to several different possible mRNA products. Each mRNA product codes for a different protein isoform; these protein isoforms differ in their peptide sequence and therefore their biological activity. It is estimated that up to 60% of human gene products undergo alternative splicing. Alternative splicing contributes to protein diversity a single gene transcript (RNA) can have tho.

please explain transcription and translationSolutionAnsTran.pdf

siennatimbok52331

Similar to Gene expression (20)

Gene expression & regulation

Gene expression system

Gene expression

Transcription in prokaryotes and eukaryotes.pdf

POST TRANSCRIPTIONAL MODOFICATION.pptx

Genes in Action

Provide an in depth description of biological information transfer (.pdf

Gene expression: Translation and Transcription

Prokaryotic vs eukaryotic 3

The molecular genetics of gene expression by : CHRISTONE JAY NOVIDA

Gene expression&regulation part ii

• Define transcription• Define translation• What are the 3 steps.pdf

Genetics

Control of gene expression ppt

Chapter 20 Molecular Genetics Lesson 2 - Genes_Transcription and Translation

Transcription

Gene expressionGene expression is the process by which the genetic.pdf

Chapter 8 Microbial GeneticsPlasm

please explain transcription and translationSolutionAnsTran.pdf

Recently uploaded

Russian Call Girls Lucknow Just Call 👉👉7877925207 Top Class Call Girl Service Available Our agency presents a selection of young, charming call girls #J11 available for bookings at Oyo Hotels. Experience high-class escort services at pocket-friendly rates, with our female escorts exuding both beauty and a delightful personality, ready to meet your desires. Whether it's Housewives,#J11 College girls, Russian girls, Muslim girls, or any other preference, we offer a diverse range of options to cater to your tastes. We provide both in-call and out-call services for your convenience. Our in-call location in Lucknow ensures cleanliness, hygiene, and 100% safety, while our out-call services offer doorstep delivery for added ease. We value your time and money, hence we kindly request pic collectors, time-passers, and bargain hunters to refrain from contacting us. Our services feature various packages at competitive rates: One shot: ₹2000/in-call, ₹5000/out-call Two shots with one girl: ₹3500/in-call, ₹6000/out-call Body to body massage with sex: ₹3000/in-call Full night for one person: ₹7000/in-call, ₹10000/out-call Full night for more than 1 person: Contact us at 🔝 7877925207 🔝. for details Operating 24/7, we serve various locations in Lucknow, including Green Park, near metro stations. For premium call girl services in Lucknow 🔝 7877925207 🔝. Thank you for considering us!

Russian Call Girls Lucknow Just Call 👉👉7877925207 Top Class Call Girl Service...

adilkhan87451

The Most Attractive Hyderabad Call Girls Kothapet 𖠋 9332606886 𖠋 Will You Miss This Chance Of Getting Into My Sexy Boobs?t Booking Contact Details WhatsApp Chat: +91-9332606886 Hyderabad Escort Service includes providing maximum physical satisfaction to their clients as well as engaging conversation that keeps your time enjoyable and entertaining. Plus they look fabulously elegant; making an impressionable Independent Escorts Hyderabad understands the value of confidentiality and discretion - they will go the extra mile to meet your needs. Simply contact them via text messaging or through their online profiles; they'd be more than delighted to accommodate any request or arrange a romantic date or fun-filled night together. We provide - Flexibility Choices and options Lists of many beauty fantasies Turn your dream into reality Perfect companionship Cheap and convenient In-call and Out-call services And many more. 01-May(Ast)

The Most Attractive Hyderabad Call Girls Kothapet 𖠋 9332606886 𖠋 Will You Mis...

chandars293

Jogeshwari ! Call Girls Service Mumbai - 450+ Call Girl Cash Payment 9004268417 Neha Thakur Booking Contact Details WhatsApp Chat: +91-9004268417 Mumbai Escort Service includes providing maximum physical satisfaction to their clients as well as engaging conversation that keeps your time enjoyable and entertaining. Plus they look fabulously elegant;$S2 making an impressionable. Independent Escorts Mumbai understands the value of confidentiality and discretion - they will go the extra mile to meet your needs. Simply contact them via text messaging or through their online profiles; they'd be more than delighted to accommodate any request or arrange a romantic date or fun-filled night together. We provide - Flexibility Choices and options Lists of many beauty fantasies Turn your dream into reality Perfect companionship Cheap and convenient In-call and Out-call services And many more.

Jogeshwari ! Call Girls Service Mumbai - 450+ Call Girl Cash Payment 90042684...

Anamika Rawat

Top Rated Bangalore Call Girls Majestic ⟟ 9332606886 ⟟ Call Me For Genuine Sex Service At Affordable Rate Booking Contact Details Whatsapp Chat :+91- 9332606886 These professional and Call girls in Bangalore can satisfy your every desire - be it sensual massage or an erotic shower, they are always more than willing to meet them. Furthermore, they pay close attention to body language cues to ensure you enjoy every minute spent in their company - both in-call and outcall services are offered. Is an excellent choice for anyone seeking an exciting and thrilling night of fun and excitement The Oberoi, Bengaluru Taj West End The Leela Palace Bengaluru JW Marriott Hotel Bengaluru The Ritz-Carlton, Bangalore Sheraton Grand Bangalore Hotel at Brigade Gateway Conrad Bengaluru ITC Gardenia, Bengaluru Renaissance Bengaluru Race Course Hotel The Lalit Ashok Bangalore 30 apr 24 (LK)

Top Rated Bangalore Call Girls Majestic ⟟ 9332606886 ⟟ Call Me For Genuine S...

narwatsonia7

Night 7k to 12k Navi Mumbai Call Girl Photo 👉 BOOK NOW 9833363713 👈 ♀️ night girls hear seeking Navi Mumbai Escorts I’m sweet , warm , beautiful and friendly, independent escort girl. My body is sexy and natural. All my pictures are recent and real. InCall and outcalls as well offer class and good service. Don’t hesitate to contact me, message me on whatssap. I’m waitting for you We Are Provide Escorts Service All OYO Hotels ,3*,4*,5* Star Hotel And Home Flat, Apartment. Guest-House. Services In -Call And Out – Call Both Are Services Available. 24Hrs. Any Time Any Where Call Whatsapp, +91-◖9833363713 ◗ Our Services and Rates: – – In call Shot 1500 Out Call Short 2000 : – In call Night 6000 Out Call Night 7000 Minimum To Maximum Range Available Only Serious And Genuine Persons Can Contact Us : +91-◖9833363713 ◗ More Information And Contact Profile Real Pic Visit Our Website City Wise Escorts Service Agency. Good Looking Cheap And Best Models Girls U Can Get Best Click On Link

Night 7k to 12k Navi Mumbai Call Girl Photo 👉 BOOK NOW 9833363713 👈 ♀️ night ...

aartirawatdelhi

💕SONAM KUMAR💕Premium Call Girls Jaipur ↘️9257276172 ↙️One Night Stand With Lonely Housewife In Top Rated Hotel WhatsApp Chat : +91-9257276172 Get More Information About Jaipur Escorts Service Jaipur Escorts offer their services to their clients at any time of day or night - including sex, travel and tours. Contact them via telephone or the internet. Hire an escort as your movie partner, date or party companion - they're sure to exceed all your expectations. Girls available 24/7 make for great companions who offer sensual body massages and drinks/food service during your time together. Find one who fits with your personality or budget - these girls have you covered.

💕SONAM KUMAR💕Premium Call Girls Jaipur ↘️9257276172 ↙️One Night Stand With Lo...

khalifaescort01

☑️░ 9630942363 ░ CALL GIRLS ░ VIP ░ ESCORT ░ SERVICES ░ AGENCY ░ 9630942363 THE GENUINE ESCORT AGENCY VIP LUXURY CALL GIRLS HIGH CLASS MODELS CALL GIRLS GENUINE ESCORT BOOK BOOK APPOINTMENT - 9630942363 THE GENUINE ESCORT AGENCY BEST VIP CALL GIRLS & ESCORTS SERVICE 9630942363 VIP CALL GIRLS ALL TYPE WOMEN AVAILABLE INCALL & OUTCALL BOTH AVAILABLE BOOK NOW 9630942363 VIP GENUINE INDEPENDENT ESCORT AGENCY VIP PRIVATE AUNTIES BEAUTIFUL LOOKING HOT AND SEXT GIRLS AND PARTY TYPE GIRLS YOU WANT SERVICE THEN CALL THIS NUMBER 9630942363 ROOM ALSO PROVIDE HOME & HOTELS SERVICE FULL SAFE AND SECURE WORK WITHOUT CONDOMS, ORAL, SUCKING, LIP TO LIP, ANAL, BACK SHOTS, SEX 69, WITHOUT BLOWJOB AND MUCH MORE FOR BOOKING 9630942363

Call Girls Hosur Just Call 9630942363 Top Class Call Girl Service Available

GENUINE ESCORT AGENCY

VIP Service Call Girls Sindhi Colony 📳 7877925207 For 18+ VIP Call Girl At Their Home & Hotels Why you Choose Us- +91-7877925207 Hyderabad Escort Service includes providing maximum physical satisfaction to their clients as well as engaging conversation that keeps your time enjoyable and entertaining. Plus they look fabulously elegant; making an impressionable I Have Extremely Beautiful Broad Minded Cute Sexy & Hot Call Girls and Escorts, We Are Located in 3* 4* 5* Hotels. Safe & Secure High Class Services Affordable Rate 100% Satisfaction, Unlimited Enjoyment. Any Time for Model/Teens Escort in Delhi High class luxury and premium escorts agency. ★ CALL US High Class Luxury and Premium Escort Service We Provide Well Educated,Royal Class Female,High-Class Escort Service Offering a Top High Class Escort Service In The & Several Nearby All Places of . A300424ST ★ To Enjoy With Hot and Sexy Girls . ★ We Are Providing :- • 3* 5* 7*Hotels Service • Hygienic Full AC Neat and Clean Rooms Avail. • Daily New Escorts Staff Available • Minimum to Maximum Range Available. HOT⇄ 7877925207 Mr Chirag ji Our escort service •BJ (Blowjob) •FK (French kissing) •Kissing with tongue •O-Level (Oral sex) •Long Silky Brown Hair •69 (69 sex) •A-Level (5 start Escort) •Dating

VIP Service Call Girls Sindhi Colony 📳 7877925207 For 18+ VIP Call Girl At Th...

jageshsingh5554

9630942363 THE GENUINE ESCORT AGENCY VIP LUXURY CALL GIRLS HIGH CLASS MODELS CALL GIRLS GENUINE ESCORT BOOK BOOK APPOINTMENT - 9630942363 THE GENUINE ESCORT AGENCY BEST VIP CALL GIRLS & ESCORTS SERVICE 9630942363 VIP CALL GIRLS ALL TYPE WOMEN AVAILABLE INCALL & OUTCALL BOTH AVAILABLE BOOK NOW 9630942363 VIP GENUINE INDEPENDENT ESCORT AGENCY VIP PRIVATE AUNTIES BEAUTIFUL LOOKING HOT AND SEXT GIRLS AND PARTY TYPE GIRLS YOU WANT SERVICE THEN CALL THIS NUMBER 9630942363 ROOM ALSO PROVIDE HOME & HOTELS SERVICE FULL SAFE AND SECURE WORK WITHOUT CONDOMS, ORAL, SUCKING, LIP TO LIP, ANAL, BACK SHOTS, SEX 69, WITHOUT BLOWJOB AND MUCH MORE FOR BOOKING 9630942363

Call Girls Ahmedabad Just Call 9630942363 Top Class Call Girl Service Available

GENUINE ESCORT AGENCY

Call Girls Raipur Just Call 9630942363 Top Class Call Girl Service Available

GENUINE ESCORT AGENCY

Russian Call Girls Service Jaipur {8445551418} ❤️PALLAVI VIP Jaipur Call Girl UKHBET Escorts Service Available ✔ All Day And Night Booking Details Are here 05-May-2024( Mj) Click On WhatsApp Chat +91-8445551418 Jaipur Escorts Make You Feel Like Royalty Jaipur Escorts catering to high profile clients are available 24-7 to meet their clients' needs and are also available for dinner dates, business conferences, foreign travel trips and clubbing activities. Their customers are contacted directly through in order to prevent risky situations from developing.is home to some of India's most beautiful women. These girls come highly educated with great backgrounds; furthermore they are extremely beautiful and will make you feel like royalty if hired for hours or days! Siya Khan | FOR BOOKING CALL Our All Services :- 1 )★ (Oral to consummation) bjnonCovered 2 )★ Special Massage 3 )★ O-Level (Oral sex) 4 )★ Blow Job; 5 )★ COB (Come On Body) 6 )★. Extraball (Have sex ordinarily) 7 )★ A-Level (5 star escort) 8 )★ Strip-bother 9 )★ BBBJ (Bareback Blowjob) 10 )★ Spending time in my rooms 11 )★ BJ (Blowjob Without a Condom) 12 )★ COF (Come On Face) 13 )★ Completion

Russian Call Girls Service Jaipur {8445551418} ❤️PALLAVI VIP Jaipur Call Gir...

parulsinha

Call Girls Tirupati Just Call 8250077686 Top Class Call Girl Service Available Easy & Fast Booking Procedure Of Tirupati Escorts Call girls are ready and waiting to provide the quality essentialness that will help ease away life's tensions Tirupati Escorts are regular companions of elite gentlemen and can take them places they could only dream of visiting. These ladies take great care in maintaining themselves to look appealing; visiting salons and spas regularly keeps them looking their best; their portfolios can be found online via an escort’s agency website. EXCITING OPTIONS AVAILABLE AT OUR INDEPENDENT ESCORTS Candlelight dinner Evening date Night clubs or parties Hotels or your flats Business trip Business party Stripping party Tirupati Escort Service are adept at performing wild anal sex, deep throat sex and cum eating - perfect for satisfying all your sexual needs and playing any game imaginable. Affordable professional assistants are readily available and ready to assist with any situation - be it romantic date or solo night. Their beautiful appearances and striking personalities will leave you speechless, while they can accompany you to restaurants or bars where their presence will surely leave an impression.

Call Girls Tirupati Just Call 8250077686 Top Class Call Girl Service Available

Dipal Arora

Top Rated Hyderabad Call Girls Erragadda ⟟ 9332606886 ⟟ Call Me For Genuine Sex Service At Affordable Rate Booking Contact Details WhatsApp Chat: +91-9332606886 Hyderabad Escort Service includes providing maximum physical satisfaction to their clients as well as engaging conversation that keeps your time enjoyable and entertaining. Plus they look fabulously elegant; making an impressionable Independent Escorts Hyderabad understands the value of confidentiality and discretion - they will go the extra mile to meet your needs. Simply contact them via text messaging or through their online profiles; they'd be more than delighted to accommodate any request or arrange a romantic date or fun-filled night together. We provide - Flexibility Choices and options Lists of many beauty fantasies Turn your dream into reality Perfect companionship Cheap and convenient In-call and Out-call services And many more. 02-May(Ast)

Top Rated Hyderabad Call Girls Erragadda ⟟ 9332606886 ⟟ Call Me For Genuine ...

chandars293

Call Girls Shimla Just Call 8617370543 Top Class Call Girl Service Available Easy & Fast Booking Procedure Of Shimla Escorts Call girls are ready and waiting to provide the quality essentialness that will help ease away life's tensions Shimla Escorts are regular companions of elite gentlemen and can take them places they could only dream of visiting. These ladies take great care in maintaining themselves to look appealing; visiting salons and spas regularly keeps them looking their best; their portfolios can be found online via an escort’s agency website. EXCITING OPTIONS AVAILABLE AT OUR INDEPENDENT ESCORTS Candlelight dinner Evening date Night clubs or parties Hotels or your flats Business trip Business party Stripping party Shimla Escort Service are adept at performing wild anal sex, deep throat sex and cum eating - perfect for satisfying all your sexual needs and playing any game imaginable. Affordable professional assistants are readily available and ready to assist with any situation - be it romantic date or solo night. Their beautiful appearances and striking personalities will leave you speechless, while they can accompany you to restaurants or bars where their presence will surely leave an impression.

Call Girls Shimla Just Call 8617370543 Top Class Call Girl Service Available

Dipal Arora

Top Rated Bangalore Call Girls Mg Road ⟟ 9332606886 ⟟ Call Me For Genuine Sex Service At Affordable Rate Booking Contact Details Whatsapp Chat :+91- 9332606886 These professional and Call girls in Bangalore can satisfy your every desire - be it sensual massage or an erotic shower, they are always more than willing to meet them. Furthermore, they pay close attention to body language cues to ensure you enjoy every minute spent in their company - both in-call and outcall services are offered. Is an excellent choice for anyone seeking an exciting and thrilling night of fun and excitement The Oberoi, Bengaluru Taj West End The Leela Palace Bengaluru JW Marriott Hotel Bengaluru The Ritz-Carlton, Bangalore Sheraton Grand Bangalore Hotel at Brigade Gateway Conrad Bengaluru ITC Gardenia, Bengaluru Renaissance Bengaluru Race Course Hotel The Lalit Ashok Bangalore 30 apr 24 (LK)

Top Rated Bangalore Call Girls Mg Road ⟟ 9332606886 ⟟ Call Me For Genuine S...

narwatsonia7

Call Girls Vasai Virar Just Call 9630942363 Top Class Call Girl Service Avail...

GENUINE ESCORT AGENCY

VIP Hyderabad Call Girls Bahadurpally 7877925207 ₹5000 To 25K With AC Room 💚😋 Call Mr Chirag Ji +91-7877925207 NORTH/SOUTH INDIANS HIGH CLASS MODELS/COLLEGE GIRLS/ HOUSEWIVES/BODY TO BODY MASSAGE, SANDWICH MASSAGE, SHOWER BATHING, BLOW JOB, LICKING, FRONT AND BACK SHOT, RUBBING, KISSING SMOOCHING ETC ETC... UNLIMITED FUN Call 24/7. Your assistance for your service... ☛ ✔✔ secure✔✔ 100% safe ✔✔ Vip Callgirl Service offers you the most beautiful High Profile sexy independent female Escorts in genuine ✔✔✔ To enjoy with hot and sexy girls ✔✔✔ A03524SW ★providing:- • Models • vip Models • Russian Models • Foreigner Models • TV Actress and Celebrities • Receptionist • Air Hostess • Call Center Working Girls/Women • Hi-Tech Co. Girls/Women • Housewife • Collage Going Girls. • Travelling Escorts. • Ramp-models • Foreigner And Many More.. ★OUR BEST SERVICES: - FOR BOOKING ★ A-Level (5 star escort) ★ Strip-tease ★ BBBJ (Bareback Blowjob)

VIP Hyderabad Call Girls Bahadurpally 7877925207 ₹5000 To 25K With AC Room 💚😋

TANUJA PANDEY

Call Girls Kurnool Just Call 8250077686 Top Class Call Girl Service Available Easy & Fast Booking Procedure Of Kurnool Escorts Call girls are ready and waiting to provide the quality essentialness that will help ease away life's tensions Kurnool Escorts are regular companions of elite gentlemen and can take them places they could only dream of visiting. These ladies take great care in maintaining themselves to look appealing; visiting salons and spas regularly keeps them looking their best; their portfolios can be found online via an escort’s agency website. EXCITING OPTIONS AVAILABLE AT OUR INDEPENDENT ESCORTS Candlelight dinner Evening date Night clubs or parties Hotels or your flats Business trip Business party Stripping party Kurnool Escort Service are adept at performing wild anal sex, deep throat sex and cum eating - perfect for satisfying all your sexual needs and playing any game imaginable. Affordable professional assistants are readily available and ready to assist with any situation - be it romantic date or solo night. Their beautiful appearances and striking personalities will leave you speechless, while they can accompany you to restaurants or bars where their presence will surely leave an impression.

Call Girls Kurnool Just Call 8250077686 Top Class Call Girl Service Available

Dipal Arora

Call Girls Guntur Just Call 8250077686 Top Class Call Girl Service Available Easy & Fast Booking Procedure Of Guntur Escorts Call girls are ready and waiting to provide the quality essentialness that will help ease away life's tensions Guntur Escorts are regular companions of elite gentlemen and can take them places they could only dream of visiting. These ladies take great care in maintaining themselves to look appealing; visiting salons and spas regularly keeps them looking their best; their portfolios can be found online via an escort’s agency website. EXCITING OPTIONS AVAILABLE AT OUR INDEPENDENT ESCORTS Candlelight dinner Evening date Night clubs or parties Hotels or your flats Business trip Business party Stripping party Guntur Escort Service are adept at performing wild anal sex, deep throat sex and cum eating - perfect for satisfying all your sexual needs and playing any game imaginable. Affordable professional assistants are readily available and ready to assist with any situation - be it romantic date or solo night. Their beautiful appearances and striking personalities will leave you speechless, while they can accompany you to restaurants or bars where their presence will surely leave an impression.

Call Girls Guntur Just Call 8250077686 Top Class Call Girl Service Available

Dipal Arora

Call Girl In Pune 👉 Just CALL ME: 9352988975 💋 Call Out Call Both With High profile Girls VIP Call Girl Pune As Enjoy Never Ending Pune VIP Call Girls is highly qualified professionals. #S10 They will offer only top quality services and never try to scam or drain you of money; in fact, they strive to give complete satisfaction so that each cent spent was worth its worth. Furthermore, these professionals pride themselves in keeping customer details confidential; any information gleaned will never be shared with any third party. Two shots with one girl: ₹4500/in-call, ₹7500/out-call Body to body massage with : ₹5500/in-call Full night for one person: ₹7000/in-call, ₹12000/out-call •𝐂𝐨𝐥𝐥𝐞𝐠𝐞 𝐆𝐢𝐫𝐥𝐬 •𝐇𝐨𝐮𝐬𝐞𝐰𝐢𝐯𝐞𝐬 •𝐇𝐢𝐠𝐡 𝐏𝐫𝐨𝐟𝐢𝐥𝐞 𝐆𝐢𝐫𝐥𝐬 •𝐑𝐚𝐦𝐩 𝐌𝐨𝐝𝐞𝐥𝐬 •𝐅𝐨𝐫𝐞𝐢𝐠𝐧𝐞𝐫 𝐆𝐢𝐫𝐥𝐬 𝐎𝐮𝐫 𝐞𝐬𝐜𝐨𝐫𝐭 𝐬𝐞𝐫𝐯𝐢𝐜𝐞𝐬 •𝐇𝐉 (𝐇𝐚𝐧𝐝 𝐉𝐨𝐛) •𝐁𝐉 (𝐁𝐥𝐨𝐰𝐣𝐨𝐛) # Completion # (Oral To Completion) # Special Massage # O-Level (Oral ) # Oral With A Noncondom) # COB (Come On Body) # Extraball (Have Many Times) # All Meetings 100%Safe Call Payal:-- 9352988975

Call Girl In Pune 👉 Just CALL ME: 9352988975 💋 Call Out Call Both With High p...

chetankumar9855

Recently uploaded (20)