1. Group II, 8-Linnaeus

2. Definition
 Gel electrophoresis separates molecules on
the basis of their charge and size.
 Gel electrophoresis is a procedure that
separates molecules on the basis of their rate
of movement through a gel under the
influence of an electrical field.
 This is the process of removing a functional
DNA fragment ( a gene) from one organism
and combining it with the DNA of another
organism to study how the gene works.

3. How does it work?
 DNA is negatively charged.
 When placed in an electrical field, DNA
will migrate toward the positive pole
(anode).
 An agarose gel is used to slow the
movement of DNA and separate by size.

4. +-
Power
H

O2

DNA

5. What is an Agarose?
 Linear polymer that comes from seaweed.
 The gel is 1% agarous and has no
electrical charge.
 Agarose was first used in biology when
Robert Koch* used it as a culture
medium for Tuberculosis bacteria in
1882