PCR explained in simple terms - A T G & C of PCR - Question and answers PCRajithnandanam
www.technologyinscience.blogspot.com
PCR - polymerase chain reaction explained in simple question answer format. Type in your doubts on PCR in the comment.
A detailed description about the basic steps involved in the - PCR - Polymerase Chain Reaction, its applications,its limitations and steps to overcome it.
PCR explained in simple terms - A T G & C of PCR - Question and answers PCRajithnandanam
www.technologyinscience.blogspot.com
PCR - polymerase chain reaction explained in simple question answer format. Type in your doubts on PCR in the comment.
A detailed description about the basic steps involved in the - PCR - Polymerase Chain Reaction, its applications,its limitations and steps to overcome it.
The methods for producing a DNA profile were developed by Alec Jeffreys and his team in 1985. Jefferys discovered that an unknown sample of DNA such as blood, hair, saliva, or semen could be analyzed and a unique DNA pattern/profile could be developed.
DNA analysis was first discovered, a process called Restriction Fragment Length Polymorphism (RFLP) was used to analyze
DNA analysis is a cornerstone technique in forensic science, used to identify individuals and establish genetic relationships. It involves extracting DNA from biological samples found at crime scenes, such as blood, hair, saliva, or skin cells, and comparing it to known samples, like those from suspects or victims.
The methods for producing a DNA profile were developed by Alec Jeffreys and his team in 1985. Jefferys discovered that an unknown sample of DNA such as blood, hair, saliva, or semen could be analyzed and a unique DNA pattern/profile could be developed.
DNA analysis was first discovered, a process called Restriction Fragment Length Polymorphism (RFLP) was used to analyze
DNA analysis is a cornerstone technique in forensic science, used to identify individuals and establish genetic relationships. It involves extracting DNA from biological samples found at crime scenes, such as blood, hair, saliva, or skin cells, and comparing it to known samples, like those from suspects or victims.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
Have you ever wanted a Ruby client API to communicate with your web service? Smithy is a protocol-agnostic language for defining services and SDKs. Smithy Ruby is an implementation of Smithy that generates a Ruby SDK using a Smithy model. In this talk, we will explore Smithy and Smithy Ruby to learn how to generate custom feature-rich SDKs that can communicate with any web service, such as a Rails JSON API.
2. Murder of Nicole Beaumont
Houston, Texas
• Nicole Beaumont was gunned
down at 10.45 a.m on 3rd
April
2003 on her front doorstep.
• An elderly neighbour saw Nicole
shot twice by a man stood at the
end of her garden path. He wore a
white t-shirt and blue jeans. He
ran off in the direction of Sankey
park, westside
• The neighbour maintains the man
looked very like Nicole’s ex-
husband Ted Scholes
3. Police seized a pair of Levi’s
blue jeans and a white T-shirt.
• The articles were found in
Scholes’ washbasket
• Police forensics upon
examining the clothes
found small specks of
blood on the t-shirt
• The police explained they
would be analysing the
white blood cells for
genetic analysis to see if
they belonged to Nicole
Beaumont
4. The police explained they would
be using a technique called
Polymerase Chain Reaction
• PCR ‘amplifies’ small
samples of DNA so
that it is easier to
analyse by
electrophoresis.
• It is essentially
making lots of copies
of the original suspect
strand for closer study
5. In a short time many copies of
the suspect strand can be made
•Each cycle can
take only a matter
of minutes .
•If the cycle takes
2 minutes a single
strand could
become 1024 new
strands in 20
minutes
7. Genetic fingerprinting
• Once a large enough
sample of DNA has been
generated using PCR it
can be analysed.
• For analysis, comparison
with a known sample is
necessary. So in the
Beaumont case police had
plenty of Nicole’s blood
to compare with the speck
from Schole’s T-shirt
8. A closer look at the compared fingerprints
from the Beaumont murder.
9. Back from the dead
• PCR has been used to re-open
murder cases.
• The body of hanged man,
James Hanratty was exhumed
40 years later to determine
beyond all doubt whether he
had committed the A6 murders
• DNA from soft tissue in the
corpse was compared to nasal
mucus found at the scene of the
crime (still on a handkerchief)
in police files
• Testing proved that the nasal
mucus was indeed Hanratty’s,
he was guilty..
10. How electrophoresis produces a
genetic fingerprint
• It’s a bit like
chromotography.
• DNA fragments are
seperated not by a solvent
but by electrodes which pull
the negatively charged
phosphate groups in DNA
through a trough of Agarose
to the positive electrode.
• The DNA can be tagged
with radioactive markers
• It can then be ‘developed’
on a photographic plate
11. Why is the DNA in fragments?
• It is cut into fragments on
purpose
• This is done using just one kind
of Restriction Endonuclease
(e.g. to cut the AATT
sequence.)
• This means you will always
have a sample of various
fragments always of the same
lengths (and so weights).
• If you take a DNA sample from
the same person years later it
too will chop up in the same
predictable way.
John’s sample 1994
(extract)
John’s sample 2004
12. Because there are many different
fragments of different lengths…
• Because they are different
sizes the fragments have
different molecular masses
• As we know larger heavier
molecules will not travel as
far as smaller lighter
molecules
• An endonuclease might cut
at the AATT sequence
• The same endonuclease
must always be used.
Why?
