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STUDY OF ADULTERANTS IN FOOD-STUFFS

INVESTIGATORY PROJECT

CHEMISTRY PROJECT WORK

Chemistry Project on STUDY OF ADULTERANTS IN FOOD

Name: Taher Shabbir Hussain
Class: XII-B
School: Indian Public School
Year: 2009-2010
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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

Certified to be the bonafide work done by
Mr. / Miss

CHEMISTRY LAB

_

__ during the year ___2009-2010__

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in the _

XII – B_________

of class

TAHER SHABBIR HUSSAIN

Date _______________________________ P.G.T. in __

CHEMISTRY ___

INDIAN PUBLIC SCHOOL

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Salmiya-Kuwait

Submitted for ALL INDIA SENIOR SCHOOL CERTIFICATE PRACTICAL
Examination held in ____CHEMISTRY LAB

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Salmiya , Kuwait.

_ at INDIAN PUBLIC SCHOOL

Examiner

Date _______________
Seal

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

I would like to express my sincere gratitude to my chemistry mentor
Mrs. Shirley Zachariah, for her vital support, guidance and encouragement -

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without which this project would not have come forth. I would also like to
express my gratitude to the lab assistant Mrs. Julie Sam for her support during

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the making of this project.

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

Page No.
6
8
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12
13
14
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Contents
Objective
Introduction
Theory
Experiment 1
Experiment 2
Experiment 3
Result
Conclusion
Bibliography

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S.No.
I.
II.
III.
IV.
V.
VI.
VII.
VIII.
IX.

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

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The Objective of this project is to study some of the
common food adulterants present in different food stuffs.

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

Adulteration in food is normally present in its most crude form;
prohibited substances are either added or partly or wholly substituted.

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Normally the contamination/adulteration in food is done either for financial
gain or due to carelessness and lack in proper hygienic condition of processing,
storing, transportation and marketing. This ultimately results that the

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consumer is either cheated or often become victim of diseases. Such types of
adulteration are quite common in developing countries or backward countries.
It is equally important for the consumer to know the common adulterants and

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their effect on health.

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

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The increasing number of food producers and the outstanding amount
of import foodstuffs enables the producers to mislead and cheat consumers.
To differentiate those who take advantage of legal rules from the ones who
commit food adulteration is very difficult. The consciousness of consumers
would be crucial. Ignorance and unfair market behavior may endanger
consumer health and misleading can lead to poisoning. So we need simple
screening tests for their detection.

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In the past few decades, adulteration of food has become one of the
serious problems. Consumption of adulterated food causes serious diseases
like cancer, diarrhoea, asthma, ulcers, etc. Majority of fats, oils and butter are
paraffin wax, castor oil and hydrocarbons. Red chilli powder is mixed with brick
powder and pepper is mixed with dried papaya seeds. These adulterants can
be easily identified by simple chemical tests.
Several agencies have been set up by the Government of India to
remove adulterants from food stuffs.

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AGMARK - acronym for agricultural marketing....this organization
certifies food products for their quality. Its objective is to promote the Grading
and Standardization of agricultural and allied commodities.

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

To detect the presence of adulterants in fat, oil and butter.

REQUIREMENTS

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Test-tube, acetic anhydride, conc. H2SO4, acetic acid, conc. HNO3.
PROCEDURE

Common adulterants present in ghee and oil are paraffin wax,
hydrocarbons, dyes and argemone oil. These are detected as follows :

(iii)

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(ii)

Adulteration of paraffin wax and hydrocarbon in vegetable ghee
Heat small amount of vegetable ghee with acetic anhydride. Droplets
of oil floating on the surface of unused acetic anhydride indicates the
presence of wax or hydrocarbons.
Adulteration of dyes in fat
Heat 1mL of fat with a mixture of 1mL of conc. sulphuric acid and
4mL of acetic acid. Appearance of pink or red colour indicates
presence of dye in fat.
Adulteration of argemone oil in edible oils
To small amount of oil in a test-tube, add few drops of conc. HNO3
and shake. Appearance of red colour in the acid layer indicates
presence of argemone oil.

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(i)

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

To detect the presence of adulterants in sugar
REQUIREMENTS

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Test-tubes, dil. HCl.
PROCEDURE

Sugar is usually contaminated with washing soda and other insoluble
substances which are detected as follows :

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(ii)

Adulteration of various insoluble substances in sugar
Take small amount of sugar in a test-tube and shake it with little
water. Pure sugar dissolves in water but insoluble impurities do not
dissolve.
Adulteration of chalk powder, washing soda in sugar
To small amount of sugar in a test-tube, add few drops of dil. HCl.
Brisk effervescence of CO2 shows the presence of chalk powder or
washing soda in the given sample of sugar.

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(i)

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

To detect the presence of adulterants in samples of chilli powder,
turmeric powder and pepper
REQUIREMENTS

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Test-tubes, conc. HCl, dil. HNO3, KI solution
PROCEDURE

(iii)

(iv)

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(ii)

Adulteration of red lead salts in chilli powder
To a sample of chilli powder, add dil. HNO3. Filter the solution and
add 2 drops of potassium iodide solution to the filtrate. Yellow ppt.
indicates the presence of lead salts in chilli powder.
Adulteration of yellow lead salts to turmeric powder
To a sample of turmeric powder add conc. HCl. Appearance of
magenta colour shows the presence of yellow oxides of lead in
turmeric powder.
Adulteration of brick powder in red chilli powder
Add small amount of given red chilli powder in beaker containing
water. Brick powder settles at the bottom while pure chilli powder
floats over water.
Adulteration of dried papaya seeds in pepper
Add small amount of sample of pepper to a beaker containing water
and stir with a glass rod. Dried papaya seeds being lighter float over
water while pure pepper settles at the bottom.

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(i)

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Common adulterants present in chilli powder, turmeric powder and
pepper are red coloured lead salts, yellow lead salts and dried papaya seeds
respectively. They are detected as follows :

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

2.

Adulteration of dyes
in fat

3.

Adulteration of
argemone oil in edible
oils
Adulteration of
various insoluble
substances in sugar

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7.

8.

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5.

OBSERVATION
Appearance of oil
floating on the
surface.

Appearance of pink
colour.
No red colour
observed

Pure sugar
dissolves in water
but insoluble
impurities do not
dissolve.

Adulteration of chalk To small amount of sugar in a
No brisk
powder, washing soda test tube, add a few drops of
effervescence
in sugar
dil. HCl.
observed.
Adeulteration of
To sample of turmeric
Appearance of
yellow lead salts to
powder, add conc. HCl.
magenta colour
turmeric powder
Adulteration of red
To a sample of chilli powder,
No yellow ppt.
lead salts in chilli
add dil. HNO3. Filter the
powder
solution and add 2 drops of
KI solution to the filtrate.
Adulteration of brick
Add small amount of given Brick powder settles
powder in chilli
red chilli powder in a beaker at the bottom while
powder
containing water.
pure chilli powder
floats over water.
Adulteration of dried Add small amount of sample Dried papaya seeds
papaya seeds in
of pepper to beaker
being lighter float
pepper
containing water and stir
over water while
with a glass rod.
pure pepper settles
at the bottom.

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4.

PROCEDURE
Heat small amount of
vegetable ghee with acetic
anhydride. Droplets of oil
floating on the surface of
unused acetic anhydride
indicate the presence of wax
or hydrocarbon.
Heat 1mL of fat with a
mixture of 1mL of conc.
H2SO4 and 4mL of acetic acid.
To small amount of oil in a
test tube, add few drops of
conc. HNO3 & shake.
Take small amount of sugar
in a test tube and shake it
with little water.

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EXPERIMENT
Adulteration of
paraffin wax and
hydrocarbon in
vegetable ghee

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EXPT. NO.
1.

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

Selection of wholesome and non-adulterated food is essential for daily
life to make sure that such foods do not cause any health hazard. It is not
possible to ensure wholesome food only on visual examination when the toxic

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contaminants are present in ppm level. However, visual examination of the
food before purchase makes sure to ensure absence of insects, visual fungus,
foreign matters, etc. Therefore, due care taken by the consumer at the time of

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purchase of food after thoroughly examining can be of great help. Secondly,
label declaration on packed food is very important for knowing the ingredients
and nutritional value. It also helps in checking the freshness of the food and

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the period of best before use. The consumer should avoid taking food from an
unhygienic place and food being prepared under unhygienic conditions. Such
types of food may cause various diseases. Consumption of cut fruits being sold

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in unhygienic conditions should be avoided. It is always better to buy certified
food from reputed shop.

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Chemistry Project on STUDY OF ADULTERANTS IN FOOD

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www.wikipedia.com

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ENCARTA ENCYCLOPEDIA 2009

www.answers.com

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www.google.com

15 | P a g e

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  • 1. om E.c iC BS STUDY OF ADULTERANTS IN FOOD-STUFFS INVESTIGATORY PROJECT CHEMISTRY PROJECT WORK Chemistry Project on STUDY OF ADULTERANTS IN FOOD Name: Taher Shabbir Hussain Class: XII-B School: Indian Public School Year: 2009-2010
  • 2. iC BS E.c om Chemistry Project on STUDY OF ADULTERANTS IN FOOD 2|P a ge
  • 3. Chemistry Project on STUDY OF ADULTERANTS IN FOOD Certified to be the bonafide work done by Mr. / Miss CHEMISTRY LAB _ __ during the year ___2009-2010__ om in the _ XII – B_________ of class TAHER SHABBIR HUSSAIN Date _______________________________ P.G.T. in __ CHEMISTRY ___ INDIAN PUBLIC SCHOOL BS E.c Salmiya-Kuwait Submitted for ALL INDIA SENIOR SCHOOL CERTIFICATE PRACTICAL Examination held in ____CHEMISTRY LAB iC Salmiya , Kuwait. _ at INDIAN PUBLIC SCHOOL Examiner Date _______________ Seal 3|P a ge
  • 4. Chemistry Project on STUDY OF ADULTERANTS IN FOOD I would like to express my sincere gratitude to my chemistry mentor Mrs. Shirley Zachariah, for her vital support, guidance and encouragement - om without which this project would not have come forth. I would also like to express my gratitude to the lab assistant Mrs. Julie Sam for her support during iC BS E.c the making of this project. 4|P a ge
  • 5. Chemistry Project on STUDY OF ADULTERANTS IN FOOD Page No. 6 8 9 10 11 12 13 14 15 BS E.c om Contents Objective Introduction Theory Experiment 1 Experiment 2 Experiment 3 Result Conclusion Bibliography iC S.No. I. II. III. IV. V. VI. VII. VIII. IX. 5|P a ge
  • 6. Chemistry Project on STUDY OF ADULTERANTS IN FOOD iC BS E.c om The Objective of this project is to study some of the common food adulterants present in different food stuffs. 6|P a ge
  • 7. iC BS E.c om Chemistry Project on STUDY OF ADULTERANTS IN FOOD 7|P a ge
  • 8. Chemistry Project on STUDY OF ADULTERANTS IN FOOD Adulteration in food is normally present in its most crude form; prohibited substances are either added or partly or wholly substituted. om Normally the contamination/adulteration in food is done either for financial gain or due to carelessness and lack in proper hygienic condition of processing, storing, transportation and marketing. This ultimately results that the E.c consumer is either cheated or often become victim of diseases. Such types of adulteration are quite common in developing countries or backward countries. It is equally important for the consumer to know the common adulterants and iC BS their effect on health. 8|P a ge
  • 9. Chemistry Project on STUDY OF ADULTERANTS IN FOOD om The increasing number of food producers and the outstanding amount of import foodstuffs enables the producers to mislead and cheat consumers. To differentiate those who take advantage of legal rules from the ones who commit food adulteration is very difficult. The consciousness of consumers would be crucial. Ignorance and unfair market behavior may endanger consumer health and misleading can lead to poisoning. So we need simple screening tests for their detection. BS E.c In the past few decades, adulteration of food has become one of the serious problems. Consumption of adulterated food causes serious diseases like cancer, diarrhoea, asthma, ulcers, etc. Majority of fats, oils and butter are paraffin wax, castor oil and hydrocarbons. Red chilli powder is mixed with brick powder and pepper is mixed with dried papaya seeds. These adulterants can be easily identified by simple chemical tests. Several agencies have been set up by the Government of India to remove adulterants from food stuffs. iC AGMARK - acronym for agricultural marketing....this organization certifies food products for their quality. Its objective is to promote the Grading and Standardization of agricultural and allied commodities. 9|P a ge
  • 10. Chemistry Project on STUDY OF ADULTERANTS IN FOOD To detect the presence of adulterants in fat, oil and butter. REQUIREMENTS om Test-tube, acetic anhydride, conc. H2SO4, acetic acid, conc. HNO3. PROCEDURE Common adulterants present in ghee and oil are paraffin wax, hydrocarbons, dyes and argemone oil. These are detected as follows : (iii) E.c BS (ii) Adulteration of paraffin wax and hydrocarbon in vegetable ghee Heat small amount of vegetable ghee with acetic anhydride. Droplets of oil floating on the surface of unused acetic anhydride indicates the presence of wax or hydrocarbons. Adulteration of dyes in fat Heat 1mL of fat with a mixture of 1mL of conc. sulphuric acid and 4mL of acetic acid. Appearance of pink or red colour indicates presence of dye in fat. Adulteration of argemone oil in edible oils To small amount of oil in a test-tube, add few drops of conc. HNO3 and shake. Appearance of red colour in the acid layer indicates presence of argemone oil. iC (i) 10 | P a g e
  • 11. Chemistry Project on STUDY OF ADULTERANTS IN FOOD To detect the presence of adulterants in sugar REQUIREMENTS om Test-tubes, dil. HCl. PROCEDURE Sugar is usually contaminated with washing soda and other insoluble substances which are detected as follows : E.c BS (ii) Adulteration of various insoluble substances in sugar Take small amount of sugar in a test-tube and shake it with little water. Pure sugar dissolves in water but insoluble impurities do not dissolve. Adulteration of chalk powder, washing soda in sugar To small amount of sugar in a test-tube, add few drops of dil. HCl. Brisk effervescence of CO2 shows the presence of chalk powder or washing soda in the given sample of sugar. iC (i) 11 | P a g e
  • 12. Chemistry Project on STUDY OF ADULTERANTS IN FOOD To detect the presence of adulterants in samples of chilli powder, turmeric powder and pepper REQUIREMENTS om Test-tubes, conc. HCl, dil. HNO3, KI solution PROCEDURE (iii) (iv) BS (ii) Adulteration of red lead salts in chilli powder To a sample of chilli powder, add dil. HNO3. Filter the solution and add 2 drops of potassium iodide solution to the filtrate. Yellow ppt. indicates the presence of lead salts in chilli powder. Adulteration of yellow lead salts to turmeric powder To a sample of turmeric powder add conc. HCl. Appearance of magenta colour shows the presence of yellow oxides of lead in turmeric powder. Adulteration of brick powder in red chilli powder Add small amount of given red chilli powder in beaker containing water. Brick powder settles at the bottom while pure chilli powder floats over water. Adulteration of dried papaya seeds in pepper Add small amount of sample of pepper to a beaker containing water and stir with a glass rod. Dried papaya seeds being lighter float over water while pure pepper settles at the bottom. iC (i) E.c Common adulterants present in chilli powder, turmeric powder and pepper are red coloured lead salts, yellow lead salts and dried papaya seeds respectively. They are detected as follows : 12 | P a g e
  • 13. Chemistry Project on STUDY OF ADULTERANTS IN FOOD 2. Adulteration of dyes in fat 3. Adulteration of argemone oil in edible oils Adulteration of various insoluble substances in sugar 6. 7. 8. 9. BS 5. OBSERVATION Appearance of oil floating on the surface. Appearance of pink colour. No red colour observed Pure sugar dissolves in water but insoluble impurities do not dissolve. Adulteration of chalk To small amount of sugar in a No brisk powder, washing soda test tube, add a few drops of effervescence in sugar dil. HCl. observed. Adeulteration of To sample of turmeric Appearance of yellow lead salts to powder, add conc. HCl. magenta colour turmeric powder Adulteration of red To a sample of chilli powder, No yellow ppt. lead salts in chilli add dil. HNO3. Filter the powder solution and add 2 drops of KI solution to the filtrate. Adulteration of brick Add small amount of given Brick powder settles powder in chilli red chilli powder in a beaker at the bottom while powder containing water. pure chilli powder floats over water. Adulteration of dried Add small amount of sample Dried papaya seeds papaya seeds in of pepper to beaker being lighter float pepper containing water and stir over water while with a glass rod. pure pepper settles at the bottom. iC 4. PROCEDURE Heat small amount of vegetable ghee with acetic anhydride. Droplets of oil floating on the surface of unused acetic anhydride indicate the presence of wax or hydrocarbon. Heat 1mL of fat with a mixture of 1mL of conc. H2SO4 and 4mL of acetic acid. To small amount of oil in a test tube, add few drops of conc. HNO3 & shake. Take small amount of sugar in a test tube and shake it with little water. om EXPERIMENT Adulteration of paraffin wax and hydrocarbon in vegetable ghee E.c EXPT. NO. 1. 13 | P a g e
  • 14. Chemistry Project on STUDY OF ADULTERANTS IN FOOD Selection of wholesome and non-adulterated food is essential for daily life to make sure that such foods do not cause any health hazard. It is not possible to ensure wholesome food only on visual examination when the toxic om contaminants are present in ppm level. However, visual examination of the food before purchase makes sure to ensure absence of insects, visual fungus, foreign matters, etc. Therefore, due care taken by the consumer at the time of E.c purchase of food after thoroughly examining can be of great help. Secondly, label declaration on packed food is very important for knowing the ingredients and nutritional value. It also helps in checking the freshness of the food and BS the period of best before use. The consumer should avoid taking food from an unhygienic place and food being prepared under unhygienic conditions. Such types of food may cause various diseases. Consumption of cut fruits being sold iC in unhygienic conditions should be avoided. It is always better to buy certified food from reputed shop. 14 | P a g e
  • 15. Chemistry Project on STUDY OF ADULTERANTS IN FOOD E.c www.wikipedia.com om ENCARTA ENCYCLOPEDIA 2009 www.answers.com iC BS www.google.com 15 | P a g e