SlideShare a Scribd company logo
Mibelle AG Biochemistry, 5033 Buchs/Switzerland, Phone +41 (0)62 836 13 45, info@mibellebiochemistry.com, www.mibellebiochemistry.com
0
30000
60000
90000
120000
150000
Control
Malus Domestica stem cell extract
0.01% 0.1%
Cellnumber
S-142/ © Mibelle Biochemistry
-50
-40
-30
-20
-10
0
Malus Domestica stem cell extract
Decrease%
S-142/ © Mibelle Biochemistry
Control 0.01% 0.1%
Dr. M. Sieber
BIONET Corp., Taipei 231, Taiwan
Sponsor:
Mibelle AG - Biochemistry, 5033 Buchs, Switzerland
Test Products
• Malus Domestica stem cell extract
Cell Line
• Umbilical cord derived stem cells
Assays
• Growth / Proliferation Test
Cells were seeded on 6-well plates (2x104
/well). The medium
was changed after a first incubation period, the cell number
was counted and pictures were taken.
• UV Exposure Damage Test
Cells were seeded on 96-well plates (5x103
/well), the medium
was changed after a first incubation period and the cells were
exposed to UV radiation afterwards. The MTS assay was
performed after 48 hours.
Results
Malus Domestica stem cell extract shows a promoting effect
on stem cell growth. It also shows a protective effect against
UV damage.
Effect of PhytoCellTecTM
Malus Domestica on Human
Stem Cells
Protection of MSC against UV DamageIncrease of MSC Cell Growth
Control 0.1% Malus Domestica
stem cell extract
S-142/ © Mibelle Biochemistry
Picture of MSC at 8th day

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Estudios sobre las celulas madres

  • 1. Mibelle AG Biochemistry, 5033 Buchs/Switzerland, Phone +41 (0)62 836 13 45, info@mibellebiochemistry.com, www.mibellebiochemistry.com 0 30000 60000 90000 120000 150000 Control Malus Domestica stem cell extract 0.01% 0.1% Cellnumber S-142/ © Mibelle Biochemistry -50 -40 -30 -20 -10 0 Malus Domestica stem cell extract Decrease% S-142/ © Mibelle Biochemistry Control 0.01% 0.1% Dr. M. Sieber BIONET Corp., Taipei 231, Taiwan Sponsor: Mibelle AG - Biochemistry, 5033 Buchs, Switzerland Test Products • Malus Domestica stem cell extract Cell Line • Umbilical cord derived stem cells Assays • Growth / Proliferation Test Cells were seeded on 6-well plates (2x104 /well). The medium was changed after a first incubation period, the cell number was counted and pictures were taken. • UV Exposure Damage Test Cells were seeded on 96-well plates (5x103 /well), the medium was changed after a first incubation period and the cells were exposed to UV radiation afterwards. The MTS assay was performed after 48 hours. Results Malus Domestica stem cell extract shows a promoting effect on stem cell growth. It also shows a protective effect against UV damage. Effect of PhytoCellTecTM Malus Domestica on Human Stem Cells Protection of MSC against UV DamageIncrease of MSC Cell Growth Control 0.1% Malus Domestica stem cell extract S-142/ © Mibelle Biochemistry Picture of MSC at 8th day