This study compared embryo development from fresh versus vitrified metaphase II oocytes after ICSI. Sibling oocytes from patients undergoing IVF were either used fresh or vitrified and later warmed. Results showed fertilization and embryo development rates up to day 2 were not affected by vitrification. Oocyte survival after warming was over 95%. Pregnancy rates were also similar between fresh and vitrified oocytes. This study provides evidence that vitrification does not impair early embryo development and can support ongoing pregnancies comparable to fresh oocytes.
In-vitro fertilization (IVF) is a process by which oocytes are fertilized by sperm outside the women’s womb, in vitro. It still represents one of the most exciting modern scientific developments and continues to have a tremendous impact on
people's lives.
Here, we will discuss all about the embryo development inside the dish.
Also we discuss which embryo to choose for transferring into female's uterus.
In vitro fertilization is a multistage procedure for preventing fertility or genetic problems with the conception of a child. The in vitro fertilization is a complex process.IVF is the most effective form of assisted reproductive technology. There are certain steps involved in the process. The best center for in-vitro fertilization is the SCI IVF Centre.
In-vitro fertilization (IVF) is a process by which oocytes are fertilized by sperm outside the women’s womb, in vitro. It still represents one of the most exciting modern scientific developments and continues to have a tremendous impact on
people's lives.
Here, we will discuss all about the embryo development inside the dish.
Also we discuss which embryo to choose for transferring into female's uterus.
In vitro fertilization is a multistage procedure for preventing fertility or genetic problems with the conception of a child. The in vitro fertilization is a complex process.IVF is the most effective form of assisted reproductive technology. There are certain steps involved in the process. The best center for in-vitro fertilization is the SCI IVF Centre.
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
The term in vitro, from the Latin meaning in glass, is used, because early biological experiments involving cultivation of tissues outside the living organism from which they came, were carried out in glass containers such as beakers, test tubes, or petri dishes.
It was while performing SUZI that a single spermatozoon accidentally penetrated into the oolemma and provided the hint that a direct sperm injection would be more efficient.
1st successful birth by ICSI took place on Jan 14, 1992.
interest in stem cells is raising in different field of medicine. The question is : is it successful in Gynecology or it is still too early to say that. The present talk may help to explore this .
EMBRYO QUALITY ASSESSMENT, WHICH TO SELECT? Rahul Sen
Traditional embryo evaluation systems are simple, non-invasive, cost-effective & mainstay in majority of IVF laboratories. Embryo selection based on combinations of morphology scores at different stages of embryonic development with time may be more effective
The term in vitro, from the Latin meaning in glass, is used, because early biological experiments involving cultivation of tissues outside the living organism from which they came, were carried out in glass containers such as beakers, test tubes, or petri dishes.
It was while performing SUZI that a single spermatozoon accidentally penetrated into the oolemma and provided the hint that a direct sperm injection would be more efficient.
1st successful birth by ICSI took place on Jan 14, 1992.
interest in stem cells is raising in different field of medicine. The question is : is it successful in Gynecology or it is still too early to say that. The present talk may help to explore this .
Hvordan fik jeg praktikpladsen?
Hvem er NlRAS?
Hvad lavede jeg i praktikperioden?
Hvilke kompetencer skal man have?
Hvad fik jeg ud af min praktik?
Hvilke gode råd kan jeg give jer?
1.Finansieringen af Skjern Å genopretning
2.Kommissorium for Skjern Å genopretningsprojektet
3.Naturstyrelsens plejeplan for Natura-2000 område nr. 68 Skjern Å
4.Plejeplansopbygning og forslag til pleje
This was the first training session I did for using Mendeley as a Reference Management software after being inducted into the Mendeley Advisors' Group. The target group for this presentation was Master's students with no prior experience of doing research or using reference management software. These students had applied for competitive grants to the Research Capacity Building Program being run by the India Research Initiative on Peri-Urban Human-Animal-Environment Health (which employs me at the time of uploading this presentation). In addition to providing them with seed funding to support their Master's theses, the Capacity Building Program also provided multiple opportunities for learning, networking and skill building, including a workshop on the Basics of EcoHealth Research Methods, in course of which this presentation was made.
November 7, 2016
For over 35 years, the “14-Day Rule,” prohibiting in vitro experimentation on embryos beyond 14 days, stood as an ethical line in the sand for embryo research around the world. Throughout the arc of the rule’s existence it had not been questioned, as scientists had been unable to grow embryos in vitro either up to, or beyond, 14 days; a practical limitation that served as a backstop to the ethical rule. However, in May 2016, labs in the U.S. and the U.K. were the first to report being able to sustain human embryos in vitro for up to 13 days. This development and other advances in in vitro research involving organized, embryo-like cellular structures have raised a number of questions about the rule, its genesis, application, and future scope. This conference convened experts in bioethics, stem cell research, embryology, and law to discuss the ethical underpinnings and future scope of the rule. Questions discussed included:
- What are the historical, ethical and scientific rationales for establishing the 14-Day Rule?
- Should the 14-Day Rule be revisited in light of recent advances?
- Should the 14-Day Rule even apply to research involving the in vitro culture of embryo-like cellular structures?
This event was free and open to the public.
This event was sponsored by the Harvard University Office of the Vice Provost for Research, the Edmond J. Safra Center for Ethics at Harvard University, and the Petrie-Flom Center for Health Law Policy, Biotechnology, and Bioethics at Harvard Law School, and the Harvard Stem Cell Institute, with support from the International Society for Stem Cell Research and the Center for Bioethics at Harvard Medical School.
View the full agenda and learn more on the website: http://petrieflom.law.harvard.edu/events/details/advances-in-in-vitro-research-and-the-14-day-rule.
Bees sting,but gives us honey.Serpent bites,but it’s poison is used in medicine for treatment of cancer.
Tigers attack,but because of their presence in forests,the poachers dare not to loot,The treasure like skin of deers,tusks of elephants,logs of trees etc.Elephant when gets enraged throws the Mahaut[rider on back] out and tramples the people assembled nearby.But their presence in the temple yard and their participation in procession and cart festival of God adds attraction to the ceremony.Elephants in some forest areas are also used to transport logs of wood.
Crows seize the pieces of bread from the hands of tiny toddlers,But they keep the environment clean by picking worms and rotten things spread around the area.
Spiders web at the corners of the walls look ugly but they catch mosquitoes and flies.Cows and bulls when enraged attack with their horns.But cows give milk and bulls draw carts and plough the fields in rural areas.When the dogs become mad they bite and humans get rabies,But they are homely and faithful in nature.The dog guards the master and his house.Monkeys are a nuisance but the trained ones entertain humans by their aerobatics.Donkeys kick with their hinder legs but they carry loads.Horses are powerful and proud.They are not honest.When humans invest them in races and use them for gambling they may ruin them, if luck fails.But they serve as means of livelihood for the poor who use them to draw carts.Fishing,rearing of hens and pigs in farms also provide employment for the humans.Birds eat the grains in fields but they pick the worms that cause the disease to the plants or burrow the plants.The dancing peacock represents pride.Swan stands for graceful movement,cocky means arrogant,Eagle’s eye represents sharp view,fox represents cunningness and wisdom.
When child learns to speak,we appreciate-” the child speaks like a parrot”.When a lady sings melodiously we appreciate-“she sings like a cuckoo’.Wild boars dug the soil and eat root vegitables like carrot,potatoes etc.Scientists say that they may be used to detect silent bombs buried underground,During second world war in Germany and other places ,where the metal detectors and police dogs have failed to find out.
RECENT ADVANCES ON TREATMENT OF FEMALE INFERTILITY.pptxasmita924867
The inability to conceive a child is most often viewed as a private matter, but public health perspectives and skills can contribute greatly to our knowledge about infertility, and the development of effective and rational public policy for prevention, access to health care, and regulation of new technologies. We offer a primer of public health aspects of infertility in an effort to encourage the broad spectrum of public health professionals to become more knowledgeable about these topics and join in the national debate about preventive strategies, cost-benefit assessment, resource allocation, and ethics.
this presentation highlights the principles of uterine and ovarian transplantation. It explores the past and examines the current status for uterine and ovarian factor infertility.
Indivisualization of Ovulation Induction - Dr Dhorepatil BharatiBharati Dhorepatil
IVF started to develop fast with the aim of maximizing pregnancy rates per cycle
Higher number of oocytes and thus more embryos
Use of unphysiological high doses of gonadotropins
Time consuming protocols
Higher costs
Patient discomfort
Higher risk of OHSS
Very high risk of multiple gestation
Optimal Timing of Oocyte Preincubation for Intra Cytoplasmic Sperm Injection ...theijes
IN Vitro Fertilization (IVF) i.e. fertilizing an oocyte with the sperm under in vitro condition is the most convincing option for treating infertility in the couples in which conception is not possible with conventional treatments. It is achieved either by co-culturing oocyte with sperms (conventional IVF) or by injecting single sperm in the cytoplasm of oocyte (Intra Cytoplasmic Sperm Injection - ICSI). The cultured embryos are then transferred from day2 to 4 (cleavage stage) or day 5 (blastocyst stage) in the uterus of the woman under treatment for implantation. The benefit of in vitro oocyte culture prior to insemination during conventional IVF has been demonstrated; however there are discrepancies about its advantage during ICSI procedure. We undertook this work to examine the effect of duration of pre-incubation on the rate of fertilization after ICSI. This work was carried out by making the retrospective analysis of data regarding oocyte pre incubation accumulated at Niramaya IVF Center during June 2010 to December 2015.ICSI cycles were categorized in to 5 different groups according to the duration of oocyte incubation period prior to ICSI as : Group I - oocytes not incubated, Group II - oocytes incubated between 1-3 hours, Group III- oocytes incubated between 3-5 hours, Group IV - oocytes incubated between 5-7 hours and Group V - oocytes incubated formore than 7 hours. It was observed that rate of fertilization varies with the duration of pre-incubation of oocyte prior to ICSI. We concluded that in vitro culture of oocyte for short duration prior to ICSI has beneficial impact on fertilization.
This is a slide on in vitro fertilization and everything you need to know about it in your medical school. All data and information are validated and extracted from authentic resources.
Embryo development of fresh versus vitrified mii oocytes after icsi
1. Embryo development of fresh
“versus” vitrified metaphase II
oocytes after ICSI: a prospective
randomized sibling-oocyte study
Human Reproduction Vol.25 No.1 pp.66-73, 2010
2009/12/24
Ian Huang
2. March 2004 to May 2009
• Italian Law No. 40 regulates ART
1. no more than 3 oocytes could be inseminated per
cycle.
2. all obtained embryos had to be transferred.
3. embryo cryopreservation and gamete donation
were forbidden.
• As a results, oocyte selection and oocyte
preservation have been a routine practice in Italian
IVF centers.
3. Recent edict of Italian Constitutional
Court (151/2009)
• 1. embryo freezing is constitutional, if
determined by the health of the patient.
• 2. the numbers of oocytes to be inseminated
is now chosen by the physician.
• The population of this study is from
2008/9/2 to 2009/3/10
4. Aim
• To compare the in vitro performance of
fresh and vitrified oocytes post-ICSI
procedure.
• Measure fertilization, PN morphology,
embryo development and embryo
morphology of fresh and vitrified sibling
oocytes.
5. Target population
• 1. < 42 yrs old
• 2. more than 6 normal MII oocytes
• 3. MESA/TESE or motile sperm count <
0.5x10^6/ml after preparation are excluded.
• 4. PB biopsy is excluded.
6. Methods
• GnRH agonist long protocol and GnRH-
antagonist protocol for stimulation.
• Oocyte collection at 35 h post-hCG.
• Denudation at 37 to 40 h post-hCG.
• Oocytes with dark cytoplasm, centrally
located granular area, giant with vacuoles,
large polar body were excluded.
7. • radom number assign for normal MII oocyte by
computer-generated random list.
• 1, 2, 3 for fresh ICSI
• others for vitrification(warmed ICSI). (4, 5, 6,
….so on)
• fresh ICSI:
maximum of 3 oocyte were inseminated, and all
obtained embryos are transferred at 44-48 h post
ICSI.
• warmed ICSI:
warmed at 36-40 h post-hCG in nature cycle,
surviving oocytes were cultured at 37℃(6%CO2,
5%O2) for 2 h before ICSI.
11. Discussion
• According to the results, embryo development up to Day 2
is not affected by vitrification procedure.
• Oocyte survival rate was higher than 95%.
• The number of oocytes degenerated during warming
procedure was negligible and did not affect the overall
fertilization rate.
• Although some, non-significant, differences in pronuclear
morphology were observed, embryo quality was similar in
the two groups.
• The percentage of top quality embryos per fertilized
oocyte was about 52% in fresh and vitrified group of
oocytes.
12. Discussion
• Four ongoing pregnancies (30.7%) were obtained
in women aged > 38 years. It cannot be excluded
that endometrium receptivity may also be involved
in successful implantation of embryos derived
from vitrified oocytes.
• By transferring embryos in a natural unstimulated
cycle, synchronization of embryo and endometrial
development can be, in fact, probably better
obtained.
13. Discussion
• This results indicate that oocyte vitrification
procedure followed by ICSI is not inferior to fresh
insemination procedure, with regard to
fertilization and embryo developmental rates.
• Moreover, ongoing clinical pregnancy is
compatible with this procedure, even with a
restricted number of oocytes available for
insemination.
• The promising clinical results obtained, in a
population of infertile patients, need to be
confirmed on a larger scale.