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‫عباده‬ ‫من‬ ‫هللا‬ ‫يخشى‬ ‫إنما‬
‫العلماء‬
“
‫المو‬ ‫فى‬ ‫للحاضرين‬ ‫بالشكر‬ ‫نتقدم‬
‫عد‬
ELISA & PCR
Preparation of ELISA
2
1
3 4
ELISA Reader
Steps For ELISA (indirect)
Enzyme-linked immunosorbent Assay
Step 1
inactivated HIV antigens
Step 2
serum antibodies
Step 3
Anti-human Ig
coupled to enzyme
Step 5 ( Measurement )
Step 4
Chromogen or substrate
PCR Polymerase Chain Reaction
Steps of PCR
Sampling
Separation of RNA
RNA to DNA RT
PCR
Denaturation of DNA
Annealing
Amplification
Final Result in gel
ELISA & PCR.ppt
ELISA & PCR.ppt

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ELISA & PCR.ppt