Efecto de la aplicación de ácido salicílico sobre parámetros fisiológicos en plantas de rábano tratadas bajo estrés salino

1. 8,7% of the world’s soils are saline
(FAO, 2021).
Context of saline soils in Colombia
Sources
Irrigation Minerals Agricultural
amendments
12,3% of the soils in Colombia are
saline (IDEAM, 2020). Salt tolerance /
adaption
Effects of salicylic acid
Effects of Salinity
Planthing
Problem

2. Radish production context in Colombia
Source: Own elaboration
90%
Of de national
production is in
Cundinamarca

3. Radish production context in Colombia

4. National production (in years)

5. Why radish?
● Short germination time, 3 to 7 days.
● Radishes have a moderate
commercial value and are grown
and consumed worldwide.
● Radish is sensitive to environmental
conditions, that makes them a
useful indicator of environmental
changes and stresses.
(Kostka-Rick,1993)
Source: Holly Osburn

6. How production is affected by salinity?
● affects soil physicochemical properties
● ecological balance
● Low economic returns
● Soil erosions.

7. Effects of salinity on plants

8. Effects of salinity on plants

9. Effects of salicylic acid on plant physiology
Sonali et al., (2022). Frente. Biosci. (Ed. histórica) , 27(11), 310

10. Effects of salicylic acid on plant physiology
Sonali et al., 2022. Frente. Biosci. (Ed. histórica) , 27(11), 310

11. General objective
Determine the effect of seed priming application of salicylic
acid on physiological parameters of radish plants (Raphanus
sativus L.) subjected to salinity stress.
Fuente: PngFree
Fuente: EOS DATA ANALYTICS

12. Specific objectives
● Determine the effect of the application of salicylic acid on parameters
associated with photosynthesis in radish plants under salinity stress.
● Determine the effect of salicylic acid application on membrane stability in
radish plants under salinity stress.
● Determine the effect of the application of salicylic acid on parameters
associated with water status in radish plants under salinity stress.
● Determine the effect of salicylic acid application on growth parameters in
radish plants under salinity stress.
● Determine the effect of salicylic acid application on development
parameters in radish plants under salinity stress.

13. Research questions
Will seed inhibition with salicylic acid mitigate salt stress effects
associated with physiological parameters in radishes?
Hypothesis
Radish plants treated with salicylic acid will present positive
responses related to growth under salt stress.

14. Materials and methods
Radish seeds variety
Crimson Giant
Pots ½ L?
Substratum
Soil + rice husk 60% Peat 40% Basket
Petri dishes Salicylic acid

15. Materials and methods
Imbibition
Preparation of mother
solution and dilutions
Sowing
Salinity
application

16. Experiment design
Completely randomized design (DCA) with 4 treatments and 4 replications
- T1. 0 mM (SA) / 0
ds/m (NaCl) / Water
- T2. 0 mM (SA) / 5
ds/m (NaCl) / Water
- T3. 0,25 mM (SA) / 5
ds/m (NaCl) / Water
- T4. 0,4 mM (SA) / 5
ds/m (NaCl) / Water

17. Fertilization plan

18. Objective
Photosynthesis
Variables to evaluate by objective
1
Objective
Membrane stability
2
Objective
Water status
3
Objective
Growth parameters
4 Objective
Development
parameters
5
Leaf temperature (°C)
Relative chlorophyll content
(SPAD)
Electrolyte loss
Relative water content
Foliar area
Dry weight
Number of Leafs
Diameter tuberous
root

19. Associated with photosynthesis
Leaf temperature (°C)
Relative chlorophyll
content (SPAD)

20. Membrane stability
Electrolyte loss

21. Water status
Relative water content
FRESH
WEIGHT
SATURATED
WEIGHT
DRY WEIGHT

22. Growth analysis
Direct growth variables Indirect growth variables
Length of aerial part Dry weight
Leaf area Leaf area index
Net assimilation rate
Relative growth rate

23. Development analysis
Number of Leafs Diameter
tuberous root

24. Analysis of data
● Software R-Studio version 12.1.4
● Analysis of variance (ANOVA) for a DCA for
independent data with a p<0.05.
● Validation of the assumptions of normality and
homoscedasticity by means of the Shapiro-Wilk
and Bartlett tests.
● Tukey's test with a p<0.05 to determine
significant differences between treatments.

25. Expected results
• Priming application of the SA developes
significative effects on the regulation of
the negative effects of salinity stress.
• Stress with NaCl affects the hydric
status of the plant, leaf area, area
under the canopy, relative chlorophyll
content (CRA), dry and fresh weight of
root and aerial part, root diameter.
• SA provides the plant with better
adaptation and mechanisms against
salt stress before and after to
germination

26. Schedule

27. Budget

28. Bibliography
Sonali Sangwan, Ahora Sheen Shameem, Shikha Yashveer, Hemender
Tanwar, Javid A. Parray, Hanuman Singh Jatav, Sushma Sharma, Himani
Punia, RZ Sayyed, Waleed Hassan Almalki, Peter Poczai. Papel del ácido
salicílico en la lucha contra el estrés por calor en las plantas:
conocimientos sobre la modulación de los procesos vitales. Frente.
Biosci. (Ed. histórica) 2022 , 27(11), 310.
https://doi.org/10.31083/j.fbl2711310
Reinhard Kostka-Rick, William J. Manning, Radish (Raphanus sativus L.): A
model for studying plant responses to air pollutants and other
environmental stresses, Environmental Pollution, Volume 82, Issue 2,
1993, Pages 107-138, ISSN 0269-7491,
https://doi.org/10.1016/0269-7491(93)90109-2.
(https://www.sciencedirect.com/science/article/pii/0269749193901092)

29. Thanks
Answers?

30. Effect of seed priming application of salicylic acid
on physiological parameters in radish (Raphanus
sativus L.) plants subjected to salt stress.
Alejandro Rojas, Victoria Loaiza, Karen Benavides, Mateo Vanegas,
Yair Contreras.
Fisiología vegetal básica.
Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
sede Bogotá