OBJECTIVES
At the endof the experiment, you should be able
to
– extract the DNA from strawberries and view it
- describe the physical appearance of the DNA of
strawberries
7.
1. Strictly followthe procedures.
2. The experiment will last for 20
minutes only.
3. If you are not sure on what to
do, ask your teacher.
4. CLEAN your table after you
finished.
Experiment
8.
Discussion
1. Describe theDNA of the
strawberry.
2. How do the components of
the salt-soapy mixture help in
extracting the DNA?
9.
Discussion Describe theDNA of the strawberry.
Some answers are
white fine, white strands
stringy cotton-like fibers
13.
Describe the DNAof the strawberry.
Some answers are
white fine, white strands
stringy cotton-like fibers
14.
Summary • Breakopen cells by smashing the
fruit
• Dissolve organelles and cell
membranes with detergent.
• Separate DNA from proteins with
salt:
• Na(+) is attracted to (–)DNA so it
bumps off the protein.
• Filter out the clumps with a
cheesecloth
• DNA precipitates in cold alcohol and
is spooled out.