How do detectives solve
mysteries of crimes if
there are no CCTV
footages and no
witnesses?
You eat DNA everyday. Do you
believe it?
All organisms have DNA.
We can only see DNA if we
use a microscope.
DNA Extraction from
Strawberry
OBJECTIVES
At the end of the experiment, you should be able
to
– extract the DNA from strawberries and view it
- describe the physical appearance of the DNA of
strawberries
1. Strictly follow the procedures.
2. The experiment will last for 20
minutes only.
3. If you are not sure on what to
do, ask your teacher.
4. CLEAN your table after you
finished.
Experiment
Discussion
1. Describe the DNA of the
strawberry.
2. How do the components of
the salt-soapy mixture help in
extracting the DNA?
Discussion Describe the DNA of the strawberry.
Some answers are
white fine, white strands
stringy cotton-like fibers
Describe the DNA of the strawberry.
Some answers are
white fine, white strands
stringy cotton-like fibers
Summary • Break open cells by smashing the
fruit
• Dissolve organelles and cell
membranes with detergent.
• Separate DNA from proteins with
salt:
• Na(+) is attracted to (–)DNA so it
bumps off the protein.
• Filter out the clumps with a
cheesecloth
• DNA precipitates in cold alcohol and
is spooled out.

DNA Extraction in Strawberry-Science.pptx

  • 1.
    How do detectivessolve mysteries of crimes if there are no CCTV footages and no witnesses?
  • 3.
    You eat DNAeveryday. Do you believe it? All organisms have DNA. We can only see DNA if we use a microscope.
  • 4.
  • 5.
    OBJECTIVES At the endof the experiment, you should be able to – extract the DNA from strawberries and view it - describe the physical appearance of the DNA of strawberries
  • 7.
    1. Strictly followthe procedures. 2. The experiment will last for 20 minutes only. 3. If you are not sure on what to do, ask your teacher. 4. CLEAN your table after you finished. Experiment
  • 8.
    Discussion 1. Describe theDNA of the strawberry. 2. How do the components of the salt-soapy mixture help in extracting the DNA?
  • 9.
    Discussion Describe theDNA of the strawberry. Some answers are white fine, white strands stringy cotton-like fibers
  • 13.
    Describe the DNAof the strawberry. Some answers are white fine, white strands stringy cotton-like fibers
  • 14.
    Summary • Breakopen cells by smashing the fruit • Dissolve organelles and cell membranes with detergent. • Separate DNA from proteins with salt: • Na(+) is attracted to (–)DNA so it bumps off the protein. • Filter out the clumps with a cheesecloth • DNA precipitates in cold alcohol and is spooled out.