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David Grawoig, Ph.D.
	
  
16	
  Royce	
  Rd	
  #1	
  
	
   Boston,	
  MA	
  02134	
  	
  
	
   (847)-­‐927-­‐9245	
  
	
   grawoig@gmail.com	
  	
  
SUMMARY
Innovative	
   scientist	
   with	
   experience	
   in	
   complex	
   laboratory	
   automation,	
   chemical	
  
biology,	
   virology,	
   drug	
   discovery,	
   and	
   bioinformatics.	
   Seeking	
   challenging	
  
opportunities	
  in	
  diagnostics,	
  drug	
  discovery,	
  and	
  synthetic	
  biology.	
  
	
  
EDUCATION
Ph.D.,	
  Chemistry,	
  University	
  of	
  North	
  Carolina-­‐Chapel	
  Hill	
   2014	
  
Dissertation:	
  	
  SAR	
  by	
  SHAPE:	
  Towards	
  small	
  molecules	
  that	
  target	
  RNA	
  
	
  
B.S.,	
  Biochemistry,	
  University	
  of	
  Wisconsin-­‐Madison	
   2007	
  
	
  
RESEARCH SKILLS
Laboratory	
  –	
  SHAPE	
  (selective	
  2'-­‐OH	
  acylation	
  analyzed	
  by	
  primer	
  extension)	
  RNA	
  
structure	
  experiments,	
  PCR,	
  DNA/RNA	
  sequencing,	
  protein/RNA	
  expression	
  &	
  
purification,	
  cell	
  culture,	
  radioligand	
  binding	
  assays,	
  western	
  blotting	
  
Assay	
   development	
   –	
   automated	
   magnetic	
   bead	
   nucleic	
   acid	
   purification,	
   dual	
  
luciferase	
   assays,	
   high-­‐throughput	
   screening	
   (design	
   and	
   execution),	
   SOP	
  
development	
  
Automation	
  –	
  Tecan	
  Freedom	
  Evo,	
  robotic	
  liquid	
  handling,	
  MCA96	
  programming	
  
Bio/chemoinformatics	
   –	
  JMP,	
  Python,	
  Schrodinger	
  (Maestro,	
  Canvas),	
  C,	
  C++,	
  Java,	
  
Python,	
  SQL,	
  UNIX	
  shell,	
  SHAPE	
  analysis	
  (ShapeFinder,	
  QuShape),	
  XRNA	
  
Data	
  analysis	
  –	
  Kaleidagraph,	
  Prism,	
  Word,	
  Excel,	
  SAS,	
  Illustrator,	
  Acrobat	
  	
  
Grant	
  writing	
  –NIH	
  R01	
  and	
  NIH	
  training	
  grants	
  
	
   	
  
RESEARCH EXPERIENCE
Predoctoral	
  Research	
  Assistant	
  	
   2009–2014	
  
Weeks	
  lab,	
  Dept.	
  of	
  Chemistry,	
  University	
  of	
  North	
  Carolina-­‐Chapel	
  Hill	
  	
   	
  	
  
Developed	
  and	
  executed	
  automated	
  screen	
  for	
  small	
  molecules	
  binding	
  viral	
  RNA.	
  
	
  
Undergraduate	
  and	
  Post-­‐graduate	
  Researcher	
   2006–2008	
  
Ruoho	
  lab,	
  Dept.	
  of	
  Pharmacology,	
  University	
  of	
  Wisconsin-­‐Madison	
  
Researched	
  membrane	
  protein	
  ligand	
  binding,	
  structure	
  and	
  function.	
  
	
  
Undergraduate	
  Researcher	
   2005–2006	
  
Cho	
  Lab,	
  Dept.	
  of	
  Chemistry,	
  University	
  of	
  Illinois	
  at	
  Chicago	
  
Expressed	
  and	
  purified	
  peripheral	
  membrane	
  proteins	
  (advisor:	
  Dr.	
  R.V.	
  Stahelin).	
  
	
  
HONORS AND AWARDS
NIH	
  Virology	
  Training	
  Grant	
  Trainee	
   2009-­‐2010	
  
UNC	
  Biological	
  and	
  Biomedical	
  Sciences	
  Program	
  Trainee	
   2008-­‐2009	
  
	
  
David Grawoig, Ph.D.	
  

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David Grawoig resume 061215

  • 1. David Grawoig, Ph.D.   16  Royce  Rd  #1     Boston,  MA  02134       (847)-­‐927-­‐9245     grawoig@gmail.com     SUMMARY Innovative   scientist   with   experience   in   complex   laboratory   automation,   chemical   biology,   virology,   drug   discovery,   and   bioinformatics.   Seeking   challenging   opportunities  in  diagnostics,  drug  discovery,  and  synthetic  biology.     EDUCATION Ph.D.,  Chemistry,  University  of  North  Carolina-­‐Chapel  Hill   2014   Dissertation:    SAR  by  SHAPE:  Towards  small  molecules  that  target  RNA     B.S.,  Biochemistry,  University  of  Wisconsin-­‐Madison   2007     RESEARCH SKILLS Laboratory  –  SHAPE  (selective  2'-­‐OH  acylation  analyzed  by  primer  extension)  RNA   structure  experiments,  PCR,  DNA/RNA  sequencing,  protein/RNA  expression  &   purification,  cell  culture,  radioligand  binding  assays,  western  blotting   Assay   development   –   automated   magnetic   bead   nucleic   acid   purification,   dual   luciferase   assays,   high-­‐throughput   screening   (design   and   execution),   SOP   development   Automation  –  Tecan  Freedom  Evo,  robotic  liquid  handling,  MCA96  programming   Bio/chemoinformatics   –  JMP,  Python,  Schrodinger  (Maestro,  Canvas),  C,  C++,  Java,   Python,  SQL,  UNIX  shell,  SHAPE  analysis  (ShapeFinder,  QuShape),  XRNA   Data  analysis  –  Kaleidagraph,  Prism,  Word,  Excel,  SAS,  Illustrator,  Acrobat     Grant  writing  –NIH  R01  and  NIH  training  grants       RESEARCH EXPERIENCE Predoctoral  Research  Assistant     2009–2014   Weeks  lab,  Dept.  of  Chemistry,  University  of  North  Carolina-­‐Chapel  Hill         Developed  and  executed  automated  screen  for  small  molecules  binding  viral  RNA.     Undergraduate  and  Post-­‐graduate  Researcher   2006–2008   Ruoho  lab,  Dept.  of  Pharmacology,  University  of  Wisconsin-­‐Madison   Researched  membrane  protein  ligand  binding,  structure  and  function.     Undergraduate  Researcher   2005–2006   Cho  Lab,  Dept.  of  Chemistry,  University  of  Illinois  at  Chicago   Expressed  and  purified  peripheral  membrane  proteins  (advisor:  Dr.  R.V.  Stahelin).     HONORS AND AWARDS NIH  Virology  Training  Grant  Trainee   2009-­‐2010   UNC  Biological  and  Biomedical  Sciences  Program  Trainee   2008-­‐2009     David Grawoig, Ph.D.