This document is Barbara de Giorgio's resume. She authorizes the use of her resume for potential future employment opportunities according to Italian law. The resume provides her personal details, education history, work experience, skills and qualifications. It lists her experience in molecular biology laboratory operations, personnel management, genetic analysis and sequencing. It also provides references that can be contacted.

1. I authorise the use of this resume for data processing aimed at developing potential future
employment opportunities, according to Italian Law D.lds 196/2003
BARBARA DE GIORGIO
Summary s
Education s
Work experiences s
Birth: 06/06/1976
Address: via Vallone 2, 27100 Pavia (PV) – Italy
Mobile: +39 3922846342 / +39 3480036301
E-mail: barbara.degiorgio@unimi.it
Status: single
Nationality: Italian
Lab-website: http://users.unimi.it/bollatilab/default.html
LinkedIn:llhttp://www.linkedin.com/pub/barbara-de-giorgio/28/238/991
Lab operations management: Design and leading molecular biology experiments, analysis of
experimental data from Sanger sequencing, Q-PCR, Genotyping and CNV analysis. Maintain
laboratory functionality, solve the majority of hardware and software instrumentation
troubleshootings and optimize the protocols.
Personnel managment: Excellent training and supervision of student and new lab members, strong
team worker able to comunicate effectively.
University of Pavia Sep 2007 – Feb 2011
Phd Pathology and Genetics.
Principal arguments of the PhD thesis: analysis and research of mutations of candidate genes
responsible for Marfan Syndrome and gene expression analysis of FBN1.
Classification: Awarded
University of Milan Sep 2000 – Mar 2007
Ms degree in Molecular Biology.
Principal arguments of the degree thesis: (Forensic Science) study of correlation between the histo-
morphological analysis of bone tissue and the genetic identification analysis.
Classification: 109/110
Postdoctoral Research Associate Sep 2013 – to date
EPIGET - Epidemiology, Epigenetics and Toxicology Lab Department of Clinical Sciences
and Community Health, Università degli Studi di Milano, Milan, Italy.
Principal tasks: Correlation with PM10 exposure, genotype and release of microvesicles in plasma.
Genotyping of cytochrome P450 genes involved in detox process.
Consultant, Sanger Sequencing Mar 2014 – July 2015
TOMA Advanced Biomedical Assay S.p.A., Busto Arsizio (MI).
Principal tasks: Consultant for Sanger Sequencing analysis on genes involved in Breast Cancer and
Lynch Sindrome (e.g. BRCA1/2, MSH2, MLH1, MSH6).
Molecular biologist Sep 2012 – Aug 2013
Multimedica S.p.A. Group _ (Cover Maternity).
Principal tasks: Detection, Quantification and Genotyping of Viral Infection (HCV, HBV, HIV,
HPV) and Bacterial Infection (Mycobacterium tuberculosis, Chlamydia trachomatis).
Detection of genetic variants in the coagulation factors (FII, FV, MTHFR).

2. I authorise the use of this resume for data processing aimed at developing potential future
employment opportunities, according to Italian Law D.lds 196/2003
Non professional work experiences s
s
Additional trainings s
s
English Course Milan, 2015
Emotional English Teaching– Milano.
Gene Expression profiling with HTS: Rome, 19
th
-22
th
October 2015
RNA-Seq data analysis.
ELIXIR-ITA & CINECA, Auditorium via dei Tizii 2/C, Rome.
Next generation sequencing (NGS) Training Verona, July 2015
on Illumina platform (NextSeq500, HiSeq1000)
Stage at Centro di Genomica Funzionale & PersonalGenomics_UniVr (Supervisor: Prof.
M. Delledonne).
Life Technologies™ QuantStudio™ 3D Monza, 14
th
May 2015
Digital PCR System Training
ThermoFisher_ Life Technologies – Monza.
English Course Milan, 2014
International House – Milano.
Next generation sequencing (NGS), non Rome, 09
th
-10
th
Jun 2014 (ECM)
invasive prenatal testing (NIPT), Real Time
PCR: novità, orientamenti, percorsi diagnostici
SIGU, Istituto CSS-Mendel.
Course of Clinical & Molecular Biology Rome, 22
nd
-26
th
Oct 2012 (ECM)
(focus on Sanger sequencing)
SPML, SIBioC Medicina di Laboratorio.
Biotechnology and Medicine Milan, 16
th
Jun 2011 (ECM)
Multimedica S.p.A. - Milano.
Molecular biologist Sep 2007 – Aug 2012
Fondazione IRCCS Policlinico S. Matteo, Pavia.
Principal tasks: Analysis and research of mutations of candidate genes responsible for rare genetic
disorders (e.g. Marfan Syndrome, Noonan Syndrome, Anderson-Fabry disease), Breast Cancer and
Cystic Fibrosis, gene expression analysis, CNV analysis, allelic discrimination and
pharmacogenetics analysis.
Forensic Scientist Nov 2005 – May 2007
Institute of Legal Medicine, Milan.
Principal tasks: study of correlation between the histo-morphological analysis of bone tissue and the
genetic identification analysis.
Sales Agent May 2007 – Aug 2007
Palazzo BenEssere (Integree Group), Rieti.
Call center operator Jun 2006 – Jul 2006
Ipsos - Milano.
Customer Service Representatative Jul 2000 – Aug 2007
Auchan S.p.A. Group, Cinisello Balsamo (MI).
Refresher course: Boss Unit at CEREF Scrl (Centro Regionale Formazione e Studi Cooperativi _
v.le Monza 12, 20127 Milan).
Manager of famaily pub Sep 1995 – Aug 2000
La tavern del Gaucho – Lucky pub, Monza.

3. I authorise the use of this resume for data processing aimed at developing potential future
employment opportunities, according to Italian Law D.lds 196/2003
Pubblications ___________________________________________________________
Partecipation to International Conferences & Advanced Schools S
s
Skills and Competences s
Number of Conferences presentation 16 Abstracts
4 Posters
Lecturer of pharmacogenomics 01
st
Oct 2011 – 03
rd
Oct 2011
Italian Federation of Cardiology.
Master Course: “Le Basi Genetiche delle Malattie Cardiovascolari e della Risposta Terapeutica”
(The Genetics Basis of Cardiovascolary Disease and the Therapeutic Response).
Lecturer of RT-Q PCR 28
th
May 2010 – 30
th
May 2010
Italian Federation of Cardiology.
Master Course: “Le Basi Genetiche delle Malattie Cardiovascolari e della Risposta Terapeutica”
(The Genetics Basis of Cardiovascolary Disease and the Therapeutic Response).
Lab Training Sep 2013 – to date
Lab of Molecular Biology, Milan.
Jun 2007 – Aug 2012
Lab of Molecular Biology, Pavia.
Technical skills
 DNA and RNA Extraction: from blood, tissues and buccal swabs with Promega
Maxwell
®
16 System - Personal Automation™ or commercial columns, and with Abbott
Molecular's m2000sp for sample preparation;
 Gene amplification: PCR and Primers design (Primer Express
®
);
 Gel electrophoresis: for PCR results analysis;
 Purifications of PCR products;
 Sanger sequencing: DNA sequencing with ABI Prism 3130 Genetic Analyzer;
 Pyrosequencing for SNPs detection and DNA methylation analysis;
 Quantitative PCR: with Applied Biosystems 7900HT Real-Time PCR System, and with
m2000rt for real-time amplification and detection;
 Amplification of autosomal and sex forensic STR: with forensic PCR commercial kits
(Promega Corporation and Applied Biosystems);
 Histological Analysis of bone: preparation and analysis of undecalcified and decalcified
sections of bone with three staining methods (EE, PAS, Feulgen);
 Use of Automated Liquid Handling: for preparation of complex PCR plate (e.g. 384 Q-
PCR plate);
 Data analysis: sequence, gene expression, genotyping and CNV analysis with ABI
software.
Computer skills
1. Susceptibility to particle health effects, miRNA and exosomes: rationale and
study protocol of the SPHERE study. BMC Public Health. 2014; 14(1): 1137.
2. Novel human pathological mutations. Gene symbol: LMNA. Disease:
cardiomyopathy, dilated with conduction defects. Hum Genet. 2009 Apr;125(3):350.
Biotechnology and Medicine Milan, 16
th
Jun 2011 (ECM)
Multimedica S.p.A. - Milano.
Real-Time PCR: 7900HT Basic Training Monza, 4
th
-5
th
Mar 2010
Lifetechnologies, Monza (MI).
Introductione to the NGS Technologies (SOLID
TM
) Milan, 19
th
Nov 2009
Università Bicocca – Milano.

4. I authorise the use of this resume for data processing aimed at developing potential future
employment opportunities, according to Italian Law D.lds 196/2003
Licences s
Referees s
Biologist register
 Section A: number AA_068993 (from2013).
Driving Licence
 European driving licence: B (from1995).
Contact person
 Prof., Valentina Bollati, EPIGET - Epidemiology, Epigenetics and Toxicology Lab
Department of Clinical Sciences and Community Health, Università degli Studi di Milano,
Milan, Italy.
Phone: +39 0250320127
Mail: valentina.bollati@unimi.it
 MD, PhD Andrea Piccinini, Institute of Legal Medicine, University of Milan, Forensic
Genetics Laboratory, via L. Mangiagalli 37, Milano, Italy.
Phone: ++39(0)2.50315706
Mail: andrea.piccinini@unimi.it
 Federico Sebastiani, Professional Services Manager EMEA, Illumina, via Senigallia18/2,
Milano Italy.
Phone: +39 3423399886
Mail: fsebastiani@illumina.com
 Luigi Da Prato, Sr. Sales Specialist Genetic Analysis Genetic Solutions Division,
ThermoFisher, Via G.B. Tiepolo 18, Monza MB, Italy.
Phone: +39 3356231753
Mail: Luigi.DaPrato@thermofisher.com
 Alessandro Bosetti, Technical Services Scientist Manager Italy, Promega, via Decembrio,
28, Milano, Italy.
Mail: alessandro.bosetti@promega.com
Barbara de Giorgio
Computer skills
 Operating systems: Microsoft Windows xp/7/8;
 Software: Microsoft Office, Internet Explorer, Mozzila Firefox;
 Applied science software: biological databases (Pubmed, Ensembl), bioinformatics tools
for genomic analysis (Blast, Sequencer, SeqScape, Copy Caller, Genescan, Genotyper).
Linguistic Knowledge
 Mother tongue: Italian;
 Other: English (good level).