This document provides an overview of chemical risk assessment and toxicology as it relates to foods and food ingredients. It discusses key concepts in toxicology like dose-response relationships, factors that influence toxicity, and the absorption, distribution, metabolism and excretion of toxicants in the body. It then explains the risk assessment process and considers acrylamide as a case study, discussing its properties, levels found in various foods, and cancer bioassay results. It also reviews epidemiology studies that found no increased cancer risk from dietary acrylamide exposure.
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displayed hyperlipidemia as shown by their elevated levels of serum and liver cholesterol, triglyceride, PL, VLDL, LDL and the reduction in the HDL level. It can be concluded that 0.5g/day of A. nervosa treatment was effective in reduction of cholesterol, PL, TG, VLDL, LDL and HDL in a dose dependant manner.
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IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
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Role of serum trace elements magnesium, copper and zinc, level in Libyan pati...iosrjce
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
The global prevalence of obesity is increasing rapidly and high dietary fat intake is major risk factor for the development of obesity. The present study was taken undertaken to evaluate the effect of Argyreia Nervosa Burn.F leaf ethanol extract on serum lipid profile in Wistar male albino rat fed with high fat diet and to compare it with a standard hyperlipidemic drug Sibutramine (10mg/kg). Fifty four health Wistar albino male rats were randomized in to 9 groups of 6 animals each. The groups were followed as follows Group I: Sham operated Normal (Normal Diet), Group II: Control (High fat diet), Group III: Sibutramine 10 mg/kg + HFD, Group IV: EEAN (100mg/kg) + HFD, Group V: EEAN (200mg/kg) +HFD, Group VI: EEAN
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displayed hyperlipidemia as shown by their elevated levels of serum and liver cholesterol, triglyceride, PL, VLDL, LDL and the reduction in the HDL level. It can be concluded that 0.5g/day of A. nervosa treatment was effective in reduction of cholesterol, PL, TG, VLDL, LDL and HDL in a dose dependant manner.
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Format: Microsoft PowerPoint (.ppt)
File size: 1.59 MB
Date posted: 4/25/2017
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Coughlin_IAFP_Food chemical risk assessment_August 2013
1. Chemical Hazards: How Do We
Translate Presence to Risk?
An Overview of Risk Analysis
James R. Coughlin, PhD CFS
President, Coughlin & Associates
Aliso Viejo, California
jrcoughlin@cox.net
www.linkedin.com/in/jamescoughlin
IAFP / Charlotte / July 29, 2013
SYMPOSIUM
“Chemical Risk Assessment 101:
A Better Understanding of a Complex
Subject Made Easier”
2. Objectives/Outline
Why is toxicology and risk assessment of
chemicals important for foods and food
ingredients?
General toxicology considerations, toxicity
evaluation and risk assessment
Key determinants of human risk
Consider “Risk-Benefit” Evaluation:
Acrylamide as case example
California Proposition 65
4. Toxicology – A Multidisciplinary Science
Chemistry
Biology
Pathology
Physiology
New Fields:
Genomics
Proteomics
Toxicogenomics
Nutrigenomics
Nutrition
Immunology
Public Health
Pharmacology
Statistics
Epidemiology
Newest:
The Microbiome
5.
6. Acute / Chronic Exposure and
Health Effects
“Acute” Exposure
A single exposure to a chemical that can result
in some form of toxicity or illness
“Chronic” Exposure
Usually a lower-dose exposure
for longer periods of time,
associated with
chronic/delayed effects
7. Factors That Influence Toxicity
Dose / duration / frequency of intake
Species / strain / age / sex
General state of health
Genetic & epigenetic factors
Nutritional status
Individual susceptibility (child, pregnant
woman, elderly, immune compromised)
Synergism / antagonism
Adaptation to the effect
8. Fate of Toxicants in Living Systems
“ADME”
Absorption - how much is absorbed by the body?
Distribution - what organs/body fluids does the
toxicant go to?
Metabolism - is it modified by enzymes in the body,
and to what extent? Activation to more toxic
compound vs. Detoxification
Excretion - how much of the toxicant (or its
metabolites) is retained and/or removed from the
body?
10. Human Relevance of Rodent Cancer Bioassays
Some eminent toxicologists have questioned the human relevance
of tumors seen in lifetime rodent bioassays, and they believe it’s
time to STOP doing chronic rodent bioassays at the “Maximum
Tolerated Dose”
We toxicologists make two possibly flawed assumptions about
chronic cancer bioassays…
Dose Extrapolation – effects seen at high rodent doses will also
occur at much lower human doses
Species Extrapolation – if cancer is seen in rodents, then cancer
probably occurs in humans
BUT…we need to understand Mechanisms and Modes of Action for
a chemical before we can use bioassay tumor results for regulatory
or warning purposes.
11. Risk Assessment Paradigm
Hazard Identification - Determination of adverse effects
caused by high intakes of the chemical (epidemiology, clinical,
animal, short-term and specialized studies)
Dose-Response Assessment
Selection of critical data set and toxic effect levels
Determination of Uncertainty or Safety Factors
Derive an Acceptable Daily Intake (ADI)
Exposure (Intake) Assessment
Evaluation of the range and distribution of human intakes
Risk Characterization
Estimation of the fraction of the population exceeding ADI
Evaluation of the magnitude of potential excess intakes.
12. Methods for Oral “RfDs”
RfDs - U.S. EPA’s Reference Dose (similar to other agencies’
Acceptable Daily Intake or “ADI”)
NOAEL - “No Observed Adverse Effect Level” – dose found
experimentally where there is an absence of adverse effects
LOAEL - “Lowest Observed Adverse Effect Level” – the lowest
experimental dose which increases the frequency or severity of
toxic effects
Uncertainty Factors - in risk assessment of chemical toxicants,
generally 10-fold increments with 100-fold as the default
Modifying Factors - magnitude depends on study weaknesses,
severity of effects, bioavailability, susceptible subpopulations
(such as diseased people, kids).
15. Acrylamide Snapshot: Chemistry and Toxicology
Occupational neurotoxin; genotoxic / mutagenic in cell cultures
Known rat carcinogen, classified as “probable human carcinogen”
Metabolized to glycidamide (an epoxide), also an animal carcinogen
Acrylamide & glycidamide can bind to DNA, amino acids and proteins
DNA adducts carcinogenic potential
Blood hemoglobin adducts biomarker of exposure
Dietary proteins may reduce acrylamide uptake in humans
Protective enzymes can detoxify acrylamide and glycidamide
Discovered by the Swedes in 2002 in hundreds of heat-processed
food products, making up about 40% of our calories.
7
16. Swedish Discovery of Acrylamide in Foods
(announced April 2002)
Tareke et al., J. Agric. Food Chem. 50: 4998-5006 (2002)
Discovered after illness investigations of tunnel workers
exposed to acrylamide as a grouting agent in 1997;
background levels of Hemoglobin-acrylamide adducts of non-
smoking Swedes were found to be elevated
Higher temperature / time / surface area increase levels:
Carbohydrate-rich foods high: 150 - 4,000 ppb
Protein-rich foods low, e.g. meats: 5 - 50 ppb
Not detected in unheated or boiled foods; 120 C is needed
Swedish adult acrylamide intake estimated to be 100 μg/day,
but now known to be much lower in most populations.
16
20. U.S. National Toxicology Program (NTP) –
Carcinogen Bioassay of Acrylamide
U.S. FDA nominated acrylamide and glycidamide for complete toxicology
testing in November 2002 for future risk assessment purposes
2-year cancer bioassay in rats and mice fed acrylamide in drinking water
(untreated control + 4 treatment doses), with ancillary studies on
metabolism, genotoxicity and toxicokinetics
NTP Technical Report No. 575 was peer-reviewed in April 2011; Panel
accepted conclusions that there was “Clear Evidence of Carcinogenicity”
in male & female rats and male & female mice
For consideration: the observed NTP tumor findings and cancer potencies
may be useful in increasing acrylamide’s acceptable risk level.
14
22. Acrylamide Risk Assessment Considerations Based
on NTP Cancer Bioassay
FAO/WHO Joint Expert Committee on Food Additives (JECFA)
acrylamide risk assessment (2010) used preliminary NTP data
on benign tumors in the rat mammary gland and mouse
Harderian gland, but these endpoints are not biologically
relevant to human risk assessment
JECFA and national authorities should reevaluate acrylamide’s
potential for human risk based on the lower incidences of
more relevant NTP malignant rat and mouse tumor endpoints
Lack of human cancer risk must be factored into any risk
assessment and risk management plans adopted by national
regulatory agencies (FDA, Health Canada, EFSA) and global
public health authorities (JECFA, Codex).
22
23. Recent Dietary Epidemiology Studies of
Acrylamide (Human Studies)
Pelucchi et al. 2011. “Exposure to Acrylamide and
Human Cancer - A Review and Meta-analysis of
Epidemiologic Studies.” Annals Oncology 22: 1487-1499.
“Conclusions: Available studies consistently suggest
a lack of an increased risk of most types of cancer
from exposure to acrylamide.”
Lipworth et al. 2012. “Review of Epidemiologic Studies
of Dietary Acrylamide Intake and the Risk of Cancer.”
Eur. J. Cancer Protection 21: 375-386.
Concluded no increased human risk, and urged that
no further epidemiology studies even be initiated.
23
24. “Acrylamide in Foods: A Review of the Science and
Future Considerations”
David R. Lineback, James R. Coughlin and Richard H. Stadler,
Ann. Rev. Food Sci. & Technol. 3: 15-35 (April 2012)
Most countries have advised consumers to follow the dietary
recommendations for a balanced diet issued by their food
regulatory and public health agencies.
The data available to date have been insufficient to warrant any
recommendation for a significant change in the dietary
recommendations because of acrylamide.
Current epidemiological and toxicological evidence are
insufficient to indicate that the amounts of acrylamide consumed
in the normal diet are likely to result in adverse human health
effects, particularly cancer.
24
25. No Significant Risk Level [NSRL] = (1 x 10-5)
Over 500 Carcinogens
MADL = No Observable Effect Level
1000
Over 300 Reproductive Toxicants [DARTs]
Exposure (µg/day), Not Concentration!
26. Acrylamide Battleground under California Prop 65
Listed in 1990 as a carcinogen; “Safe Harbor” level = 0.2 μg/day; must
stay below this level to avoid cancer warnings; if you can detect it, even a
1-ounce serving of any food exceeds this level
French fries: Attorney General sued and settled case (2008) against
frozen fries/tater tots demanding a 50% reduction in levels; fast-food
restaurant fries have had cancer warnings posted for years
Potato chips: AG settled (2008) the case against chip manufacturers;
agreement to cut levels to 275 ppb by end of 2011 (20 - 85% reductions) to
avoid warnings; no warnings currently being given
Cereals: Private “bounty hunter” lawyers sued cereal manufacturers in
2009; case is still pending
Coffee: “Bounty Hunter” sued coffee shops in 2010 over brewed coffee;
10 x 10-inch cancer warning placards have been posted; another case
now in court against over 120 coffee roasters for packaged roast coffees.
26
28. Food and Chemical Safety Issues
We usually test individual food chemicals, not the whole
foods or beverages (except with epidemiology)
For whole foods, we must identify biologically active toxic
component(s)
Must determine appropriate mechanism of action of
specific chemicals (carcinogens, reproductive toxicants)
Key importance of dose-response relationships
Interactions with diet/nutrients, environment & drugs
Explore sensitive segments of population (young, aged)
Risk/Benefit Assessment is crucial need:
Goal: NO “significant or unreasonable” risk!!
31. “Benefit-Risk Evaluation” to Assess the Safety of
Foods Containing Toxicants and Carcinogens
I believe we’ve been doing it the WRONG WAY for
decades, by simply evaluating the risk of individual
chemicals one by one in a food
Going forward, I believe the RIGHT WAY is to evaluate
the safety of the whole food by comparing its risks vs.
benefits using the “Holistic Approach”
Various “Benefit-Risk Evaluations” and regulatory
guidance documents have recently been published in
the U.S. [FDA’s “Mercury in Fish” evaluation] and
Europe [EFSA, ILSI Europe].
31