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Compressed samples –
what they are and how to
prevent them
Elizabeth Davidson
Deputy Director for Newborn Screening
South East Thames Newborn Screening Laboratory
COMPRESSED/DAMAGED CARDS
Repeats – quick review of all the codes
What is a compressed/damaged card?
Why are compressed/damaged cards so
important for the lab?
NEWBORN BLOODSPOT SCREENING
Clinical repeats
<72hrs after transfusion
Borderline CHT result
Inconclusive CF result
Pre-term (GA <32 weeks)
Avoidable repeats
demographic errors
bloodspot collection errors
post collection errors
NATIONAL REPORTING SUBSTATUS CODES
Clinical repeats
0302 Too soon after transfusion (<72hrs)
0314 Sickle – too premature for testing
0315 Pre-term repeat (Day 28 or day of discharge)
0316 Borderline CHT result
0317 Inconclusive CF result
NATIONAL REPORTING SUBSTATUS CODES
Demographic errors
0308 NHS number missing/not accurately recorded
No NHS number, incorrect NHS number,
twins demographic confusion/mismatch
0309 Date of sample missing/not accurately recorded
No DOC, illegible DOC, impossible DOC
0310 Date of birth not accurately matched
No DOB, inaccurate DOB (against NBSFS),
illegible DOB
NATIONAL REPORTING SUBSTATUS CODES
Bloodspot collection errors
0301 Too young for reliable screening
Collected before Day 5,
4 spots on pre-Tx card
0303 Insufficient sample
Not enough blood to complete screening,
spots too small to punch
0304 Incorrect blood application
Multi-spotted, spotted both sides
NATIONAL REPORTING SUBSTATUS CODES
Bloodspot collection errors
0305 Compressed/damaged
Many causes
0306 Day 0 and Day 5 on same card
0307 Possible faecal contamination
Discrepant results for CF screen
0311 Expired card used
NATIONAL REPORTING SUBSTATUS CODES
Post collection errors
0312 > 14 days in transit, too old for analysis
0313 Damaged in transit
Torn outer envelope,
water damaged/wet outer envelope
0305 COMPRESSED/DAMAGED
Bloodspot collection errors
0305 Compressed/damaged
compressed
incomplete drying
scratched/abraded/ridged
liquid/water damage/contamination
discoloured spots
BLOODSPOTS FROM THE LAB PERSPECTIVE
MOST PEOPLE SEE THE
CARD AS FLAT
THE LAB LOOKS AT
THE CARD IN 3D
BLOODSPOTS – TESTING THE SAMPLE
This punch needs to contain the
same amount of blood every time
The lab takes part of
the sample using a
high tech equivalent
of a hole punch
BLOODSPOTS – WHY COMPRESSED MATTERS
Compressed cardRoutine card
BLOODSPOTS – WHY WET CARDS MATTER
When a wet card is put into a glassine
envelope…
…blood is drawn back out of the card
BLOODSPOTS – WHY WET CARDS MATTER
Routine card Card which was wet
Does it really matter about that
tiny bit of blood?
IT COULD MATTER FOR THIS BABY….
TAKE HOME MESSAGES
Let the cards dry before putting them into the
glassine envelopes. Cards take longer to dry if
it is cold or raining.
If the glassine envelope is stained please leave
it with the card – the lab needs to know.
A screen positive baby could be missed because
of that tiny bit of missing blood.
Any Questions?

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Compressed samples

  • 1. Compressed samples – what they are and how to prevent them Elizabeth Davidson Deputy Director for Newborn Screening South East Thames Newborn Screening Laboratory
  • 2. COMPRESSED/DAMAGED CARDS Repeats – quick review of all the codes What is a compressed/damaged card? Why are compressed/damaged cards so important for the lab?
  • 3. NEWBORN BLOODSPOT SCREENING Clinical repeats <72hrs after transfusion Borderline CHT result Inconclusive CF result Pre-term (GA <32 weeks) Avoidable repeats demographic errors bloodspot collection errors post collection errors
  • 4. NATIONAL REPORTING SUBSTATUS CODES Clinical repeats 0302 Too soon after transfusion (<72hrs) 0314 Sickle – too premature for testing 0315 Pre-term repeat (Day 28 or day of discharge) 0316 Borderline CHT result 0317 Inconclusive CF result
  • 5. NATIONAL REPORTING SUBSTATUS CODES Demographic errors 0308 NHS number missing/not accurately recorded No NHS number, incorrect NHS number, twins demographic confusion/mismatch 0309 Date of sample missing/not accurately recorded No DOC, illegible DOC, impossible DOC 0310 Date of birth not accurately matched No DOB, inaccurate DOB (against NBSFS), illegible DOB
  • 6. NATIONAL REPORTING SUBSTATUS CODES Bloodspot collection errors 0301 Too young for reliable screening Collected before Day 5, 4 spots on pre-Tx card 0303 Insufficient sample Not enough blood to complete screening, spots too small to punch 0304 Incorrect blood application Multi-spotted, spotted both sides
  • 7. NATIONAL REPORTING SUBSTATUS CODES Bloodspot collection errors 0305 Compressed/damaged Many causes 0306 Day 0 and Day 5 on same card 0307 Possible faecal contamination Discrepant results for CF screen 0311 Expired card used
  • 8. NATIONAL REPORTING SUBSTATUS CODES Post collection errors 0312 > 14 days in transit, too old for analysis 0313 Damaged in transit Torn outer envelope, water damaged/wet outer envelope
  • 9. 0305 COMPRESSED/DAMAGED Bloodspot collection errors 0305 Compressed/damaged compressed incomplete drying scratched/abraded/ridged liquid/water damage/contamination discoloured spots
  • 10.
  • 11. BLOODSPOTS FROM THE LAB PERSPECTIVE MOST PEOPLE SEE THE CARD AS FLAT THE LAB LOOKS AT THE CARD IN 3D
  • 12. BLOODSPOTS – TESTING THE SAMPLE This punch needs to contain the same amount of blood every time The lab takes part of the sample using a high tech equivalent of a hole punch
  • 13. BLOODSPOTS – WHY COMPRESSED MATTERS Compressed cardRoutine card
  • 14. BLOODSPOTS – WHY WET CARDS MATTER When a wet card is put into a glassine envelope… …blood is drawn back out of the card
  • 15. BLOODSPOTS – WHY WET CARDS MATTER Routine card Card which was wet
  • 16. Does it really matter about that tiny bit of blood?
  • 17. IT COULD MATTER FOR THIS BABY….
  • 18. TAKE HOME MESSAGES Let the cards dry before putting them into the glassine envelopes. Cards take longer to dry if it is cold or raining. If the glassine envelope is stained please leave it with the card – the lab needs to know. A screen positive baby could be missed because of that tiny bit of missing blood.