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Colorado's Marijuana Testing 
Requirements Webinar 
Dr. Andy LaFrate, PhD 
President, Charas Scientific 
August 27th, 2014
Overview 
• Introduction 
• Testing Categories 
• Potency 
• Contaminants 
• Testing Requirements 
• Services offered by Charas Scientific 
• Summary and Future Webinars
Introduction: Andy LaFrate 
• PhD chemist with over a decade of lab experience 
• Began working as consultant in Colorado’s marijuana 
industry in 2012 
• Participant on several working groups to determine 
marijuana regulations in Colorado 
• Employed at one of the largest MIPs in Colorado 
• Left to found Charas Scientific in February 2014
Introduction: Charas Scientific 
• State-licensed marijuana testing and research lab 
• Located in Denver, Colorado 
• Performs cannabinoid potency testing and analysis 
for chemical and biological contaminants 
• “More than Just a Testing Lab” 
• Contract R&D, Strategic Partnerships 
• Consulting and Production Optimization Services
From the Industry, 
For the Industry. 
• Charas Scientific is a product of Colorado’s marijuana 
industry and brings a fresh approach to testing 
• We understand cannabis at the molecular level and 
empower our clients to use that knowledge 
• Charas delivers more than just results, we offer solutions to 
problems that we find in testing 
• Our team of scientists innovate new products and methods 
to take cannabis to the next level
Potency Testing 
• All retail marijuana including flower, 
concentrates and edibles must be 
tested for potency prior to sale 
• Product must be labelled with potency 
test results 
• Cannabinoids required: 
• THC, THCA, CBD, CBDA, CBN
Chemical Contaminant Testing 
• Some chemical contaminant testing is 
mandatory, and some is random. 
• The testing rules are dynamic, so it is 
important to stay current on them to avoid 
violations 
• Current Mandatory Testing (August 2014) 
• Residual Solvents 
• Current Random Testing 
• Pesticides and Heavy Metals
Residual Solvent Testing 
• All concentrates made with chemical 
solvents must be tested for residuals 
• Solvents allowed for use: propane, 
butane, acetone, ethanol, isopropanol, 
water, heptane and CO2 
• Current Approved Solvent Limits 
• Butane < 800 ppm (parts per million) 
• Heptane < 500 ppm
Biological Contaminant Testing 
• Biological contaminant testing is 
required for most retail marijuana 
including flower, edibles and some 
concentrates 
• Must test for harmful E. coli, 
Salmonella and Aspergillus 
• Current limits for all microbes are 
<1 CFU (colony forming unit)
Who Must Test? 
• All retail marijuana products must 
be tested prior to sale by the 
producer 
• Growers AKA OPCs 
• Infused product and extraction 
companies AKA MIPs 
• Retail stores are generally not 
required to test products
How Often Do You Test? 
• Every harvest batch and production batch must be tested for potency 
• Pooling of packages or harvest and production batches produced within 7 
days is allowed for contaminant testing 
• Validation program is designed to reduce testing burden on producers 
• Once validated, producers only need to test 10% of batches for 
contaminants
OPC Validation 
• Cultivation Facilities (OPCs) can be 
validated to reduce testing burden 
• Potency: Test four harvest batches 
produced three weeks apart within six 
months 
• Contaminants: Test every harvest 
batch for 12 weeks, minimum of 12 
batches separated by 6 days
MIP Validation 
• Marijuana Infused Product companies 
(MIPs) can be validated to reduce 
testing burden 
• Potency: Every batch of concentrate 
and edible must be tested 
• Contaminants: Every batch within 4 
week period, minimum of 4 batches 
separated by 6 day period
What We Do at Charas 
• Team of scientists specialized in analysis and 
research 
• Full service, State-licensed marijuana testing lab 
• Potency Testing 
• Residual Solvent and Terpene Testing 
• Microbial Testing 
• Pesticide Testing 
• Heavy Metals Testing (coming early 2015) 
• Contract R&D, consulting and production 
optimization services
Summary 
• Colorado’s marijuana testing laws are complex and 
dynamic, and understanding them is critical 
• Potency testing is required now for all retail 
products 
• Contaminant testing will begin October 2014 
• Medical marijuana testing will likely begin in 2015 
• Validation program allows producers to decrease 
testing 
• Understanding test results and taking action to fix 
problems is key to success in competitive market
Resources & Contact 
• Colorado Marijuana Enforcement Division (MED): 
www.colorado.gov/revenue/medicalmarijuana 
• Colorado Retail Marijuana Rules: 
R700 and R1500 Series are focused on testing 
• Charas Scientific: 
www.charasscientific.com 
• Andy LaFrate – President, Charas Scientific: 
andy@charasscientific.com 720.467.8015
Future Webinar Topics 
• How Do We Test? 
• A series of webinars focused on specific testing methods for 
potency and contaminants such as HPLC, GC and qPCR 
• Testing Validation 
• Dedicated to helping producers understand the validation 
process to reduce testing and save money 
• Consulting and Production Optimization 
• Highlights how Charas Scientific helps clients understand test 
results and empowers them to use that knowledge to better 
their business.
Thank 
you!

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Colorado's Marijuana Testing Requirements Webinar

  • 1. Colorado's Marijuana Testing Requirements Webinar Dr. Andy LaFrate, PhD President, Charas Scientific August 27th, 2014
  • 2. Overview • Introduction • Testing Categories • Potency • Contaminants • Testing Requirements • Services offered by Charas Scientific • Summary and Future Webinars
  • 3. Introduction: Andy LaFrate • PhD chemist with over a decade of lab experience • Began working as consultant in Colorado’s marijuana industry in 2012 • Participant on several working groups to determine marijuana regulations in Colorado • Employed at one of the largest MIPs in Colorado • Left to found Charas Scientific in February 2014
  • 4. Introduction: Charas Scientific • State-licensed marijuana testing and research lab • Located in Denver, Colorado • Performs cannabinoid potency testing and analysis for chemical and biological contaminants • “More than Just a Testing Lab” • Contract R&D, Strategic Partnerships • Consulting and Production Optimization Services
  • 5. From the Industry, For the Industry. • Charas Scientific is a product of Colorado’s marijuana industry and brings a fresh approach to testing • We understand cannabis at the molecular level and empower our clients to use that knowledge • Charas delivers more than just results, we offer solutions to problems that we find in testing • Our team of scientists innovate new products and methods to take cannabis to the next level
  • 6. Potency Testing • All retail marijuana including flower, concentrates and edibles must be tested for potency prior to sale • Product must be labelled with potency test results • Cannabinoids required: • THC, THCA, CBD, CBDA, CBN
  • 7. Chemical Contaminant Testing • Some chemical contaminant testing is mandatory, and some is random. • The testing rules are dynamic, so it is important to stay current on them to avoid violations • Current Mandatory Testing (August 2014) • Residual Solvents • Current Random Testing • Pesticides and Heavy Metals
  • 8. Residual Solvent Testing • All concentrates made with chemical solvents must be tested for residuals • Solvents allowed for use: propane, butane, acetone, ethanol, isopropanol, water, heptane and CO2 • Current Approved Solvent Limits • Butane < 800 ppm (parts per million) • Heptane < 500 ppm
  • 9. Biological Contaminant Testing • Biological contaminant testing is required for most retail marijuana including flower, edibles and some concentrates • Must test for harmful E. coli, Salmonella and Aspergillus • Current limits for all microbes are <1 CFU (colony forming unit)
  • 10. Who Must Test? • All retail marijuana products must be tested prior to sale by the producer • Growers AKA OPCs • Infused product and extraction companies AKA MIPs • Retail stores are generally not required to test products
  • 11. How Often Do You Test? • Every harvest batch and production batch must be tested for potency • Pooling of packages or harvest and production batches produced within 7 days is allowed for contaminant testing • Validation program is designed to reduce testing burden on producers • Once validated, producers only need to test 10% of batches for contaminants
  • 12. OPC Validation • Cultivation Facilities (OPCs) can be validated to reduce testing burden • Potency: Test four harvest batches produced three weeks apart within six months • Contaminants: Test every harvest batch for 12 weeks, minimum of 12 batches separated by 6 days
  • 13. MIP Validation • Marijuana Infused Product companies (MIPs) can be validated to reduce testing burden • Potency: Every batch of concentrate and edible must be tested • Contaminants: Every batch within 4 week period, minimum of 4 batches separated by 6 day period
  • 14. What We Do at Charas • Team of scientists specialized in analysis and research • Full service, State-licensed marijuana testing lab • Potency Testing • Residual Solvent and Terpene Testing • Microbial Testing • Pesticide Testing • Heavy Metals Testing (coming early 2015) • Contract R&D, consulting and production optimization services
  • 15. Summary • Colorado’s marijuana testing laws are complex and dynamic, and understanding them is critical • Potency testing is required now for all retail products • Contaminant testing will begin October 2014 • Medical marijuana testing will likely begin in 2015 • Validation program allows producers to decrease testing • Understanding test results and taking action to fix problems is key to success in competitive market
  • 16. Resources & Contact • Colorado Marijuana Enforcement Division (MED): www.colorado.gov/revenue/medicalmarijuana • Colorado Retail Marijuana Rules: R700 and R1500 Series are focused on testing • Charas Scientific: www.charasscientific.com • Andy LaFrate – President, Charas Scientific: andy@charasscientific.com 720.467.8015
  • 17. Future Webinar Topics • How Do We Test? • A series of webinars focused on specific testing methods for potency and contaminants such as HPLC, GC and qPCR • Testing Validation • Dedicated to helping producers understand the validation process to reduce testing and save money • Consulting and Production Optimization • Highlights how Charas Scientific helps clients understand test results and empowers them to use that knowledge to better their business.