This document provides biographical and professional information about Christine Roos Montague. It includes her education, professional experience, research skills, honors, memberships, and publications. She has a Ph.D. in Biomedical Engineering from the University of Virginia and over 25 years of experience in biomedical research, including positions at Cornell University, Ohio State University, and Johns Hopkins University. Her research has focused on areas such as vascular biology, nitric oxide, and mitochondrial function.
This document discusses tolerance in renal transplantation. It begins by explaining that the adaptive immune system responds to transplanted organs by recognizing HLA molecules from the donor, triggering an alloimmune response. There are two mechanisms of tolerance: central tolerance occurs in the thymus, while peripheral tolerance involves inactivation of T lymphocytes in tissues. The document then reviews studies showing that splenectomy or splenosis can induce clinical tolerance to renal transplants in animal models by impacting antibody levels, regulatory T cells, and microchimerism. However, attempts to achieve robust tolerance through similar protocols in humans have largely failed due to the complexity of the transplantation immunobiology.
This document summarizes several studies on Fibroblast Growth Factor 23 (FGF23) and its relationship to outcomes in kidney disease. Elevated FGF23 levels were found to independently predict mortality and progression of chronic kidney disease. A prospective study of over 3,800 patients with stages 2-4 CKD found that higher FGF23 levels correlated with worse kidney function and were a strong independent risk factor for death, even after adjusting for other risk factors. However, elevated FGF23 predicted end-stage renal disease only before adjusting for estimated GFR and other kidney risk factors.
Cystic fibrosis is a genetic disease caused by mutations in the CFTR gene. Gene therapy aims to insert a normal copy of this gene to restore function. While conceptually simple, gene therapy for cystic fibrosis has proven difficult in practice due to biological barriers and challenges with delivery vectors. Early clinical trials used adenoviral vectors administered to the nose with limited success. Subsequent trials delivered vectors directly to the lungs but were also unsuccessful. Recent trials have focused on non-viral vectors which allow for repeated administration and show promise, though further development is still needed to achieve an effective treatment through gene therapy.
This study aims to search for genetic and proteomic risk factors and protective factors associated with coronary heart disease (CHD) in order to develop new diagnostic techniques and therapies. The study will analyze gene expression patterns in peripheral blood monocytes and perform proteomics analysis of blood serum from five patient groups: 1) those with heart attack and risk factors, 2) those with heart attack without risk factors, 3) young individuals with risk factors but no heart attack, 4) elderly individuals with risk factors but no heart attack, and 5) healthy elderly individuals without risk factors. Gene expression profiles will be obtained using microarray analysis and validated with real-time PCR. Differentially expressed genes and proteins may help identify new targets for preventing and
This document discusses genomic oncology and personalized medicine, using lung cancers as a model. It summarizes several key technologies that enable genomic oncology like cDNA microarrays, array CGH, and next generation sequencing. It provides examples of how these technologies have been used to classify cancers like diffuse large B-cell lymphoma and myelodysplastic syndrome, and identify genetic mutations that can guide targeted therapies for cancers like EGFR-mutated lung cancer.
This study aims to investigate the functional relationship between the rs1111875 single nucleotide polymorphism (SNP) and type 2 diabetes susceptibility by determining the effect of the SNP on the expression of the HHEX and IDE genes. The study will generate isogenic beta cell lines that differ only in their rs1111875 genotype using CRISPR/Cas9 genome editing. Gene expression analysis will then be performed on the cell lines using TaqMan assays to measure relative expression of HHEX and IDE and determine if the risk allele of rs1111875 alters their expression levels, which could provide insight into how this SNP contributes to type 2 diabetes risk.
This document summarizes a study examining the role of tropomyosin-related kinase B (TrkB) in metastatic pancreatic cancer. The study found that TrkB was overexpressed in highly metastatic pancreatic cancer cells compared to parental cells. TrkB overexpression correlated with perineural invasion, positive retroperitoneal margins, and shorter time to liver metastasis in patient samples. The metastatic cells also showed increased activation of ERK1/2 and increased expression of IL-8 and VEGF, which are involved in invasion and metastasis. This suggests TrkB overexpression may promote the aggressive growth and metastasis of pancreatic cancer by activating signaling pathways and increasing expression of genes involved in these processes. TrkB may therefore be a novel therapeutic target for pancreatic cancer.
This document discusses tolerance in renal transplantation. It begins by explaining that the adaptive immune system responds to transplanted organs by recognizing HLA molecules from the donor, triggering an alloimmune response. There are two mechanisms of tolerance: central tolerance occurs in the thymus, while peripheral tolerance involves inactivation of T lymphocytes in tissues. The document then reviews studies showing that splenectomy or splenosis can induce clinical tolerance to renal transplants in animal models by impacting antibody levels, regulatory T cells, and microchimerism. However, attempts to achieve robust tolerance through similar protocols in humans have largely failed due to the complexity of the transplantation immunobiology.
This document summarizes several studies on Fibroblast Growth Factor 23 (FGF23) and its relationship to outcomes in kidney disease. Elevated FGF23 levels were found to independently predict mortality and progression of chronic kidney disease. A prospective study of over 3,800 patients with stages 2-4 CKD found that higher FGF23 levels correlated with worse kidney function and were a strong independent risk factor for death, even after adjusting for other risk factors. However, elevated FGF23 predicted end-stage renal disease only before adjusting for estimated GFR and other kidney risk factors.
Cystic fibrosis is a genetic disease caused by mutations in the CFTR gene. Gene therapy aims to insert a normal copy of this gene to restore function. While conceptually simple, gene therapy for cystic fibrosis has proven difficult in practice due to biological barriers and challenges with delivery vectors. Early clinical trials used adenoviral vectors administered to the nose with limited success. Subsequent trials delivered vectors directly to the lungs but were also unsuccessful. Recent trials have focused on non-viral vectors which allow for repeated administration and show promise, though further development is still needed to achieve an effective treatment through gene therapy.
This study aims to search for genetic and proteomic risk factors and protective factors associated with coronary heart disease (CHD) in order to develop new diagnostic techniques and therapies. The study will analyze gene expression patterns in peripheral blood monocytes and perform proteomics analysis of blood serum from five patient groups: 1) those with heart attack and risk factors, 2) those with heart attack without risk factors, 3) young individuals with risk factors but no heart attack, 4) elderly individuals with risk factors but no heart attack, and 5) healthy elderly individuals without risk factors. Gene expression profiles will be obtained using microarray analysis and validated with real-time PCR. Differentially expressed genes and proteins may help identify new targets for preventing and
This document discusses genomic oncology and personalized medicine, using lung cancers as a model. It summarizes several key technologies that enable genomic oncology like cDNA microarrays, array CGH, and next generation sequencing. It provides examples of how these technologies have been used to classify cancers like diffuse large B-cell lymphoma and myelodysplastic syndrome, and identify genetic mutations that can guide targeted therapies for cancers like EGFR-mutated lung cancer.
This study aims to investigate the functional relationship between the rs1111875 single nucleotide polymorphism (SNP) and type 2 diabetes susceptibility by determining the effect of the SNP on the expression of the HHEX and IDE genes. The study will generate isogenic beta cell lines that differ only in their rs1111875 genotype using CRISPR/Cas9 genome editing. Gene expression analysis will then be performed on the cell lines using TaqMan assays to measure relative expression of HHEX and IDE and determine if the risk allele of rs1111875 alters their expression levels, which could provide insight into how this SNP contributes to type 2 diabetes risk.
This document summarizes a study examining the role of tropomyosin-related kinase B (TrkB) in metastatic pancreatic cancer. The study found that TrkB was overexpressed in highly metastatic pancreatic cancer cells compared to parental cells. TrkB overexpression correlated with perineural invasion, positive retroperitoneal margins, and shorter time to liver metastasis in patient samples. The metastatic cells also showed increased activation of ERK1/2 and increased expression of IL-8 and VEGF, which are involved in invasion and metastasis. This suggests TrkB overexpression may promote the aggressive growth and metastasis of pancreatic cancer by activating signaling pathways and increasing expression of genes involved in these processes. TrkB may therefore be a novel therapeutic target for pancreatic cancer.
This document summarizes research investigating the role of the transcription factor Irx3 in regulating angiogenesis. The key findings are:
1) Irx3 expression is elevated in human microvascular endothelial cells in response to VEGF stimulation.
2) Genetic manipulation experiments showed that Irx3 promotes endothelial cell migration, chemotaxis, invasion, and tubulogenesis.
3) Irx3 overexpression increased the expression of Dll4, suggesting an increase in endothelial tip cell population.
4) Knockdown experiments identified Hey1, a downstream mediator of Notch signaling, as a negative regulator of Irx3 expression in response to VEGF.
So in summary, this research identifies Irx
This document discusses organ transplantation. It defines different types of transplants including autograft, allograft, isograft, and xenograft. It describes the most common transplants as kidney, heart, and liver transplants. For red blood cell transplants, the success is due to RBCs not expressing MHC antigens. It also discusses immune responses after transplantation, including acute and chronic rejection, and the use of immunosuppressive drugs for induction, maintenance, and specific therapy to reduce rejection.
The VLDLR attenuates house dust mite-induced airway inflammation by suppressing dendritic cell-mediated adaptive immune responses. Vldlr-/- mice challenged with house dust mite had increased eosinophilic and lymphocytic airway inflammation, higher Th2 cytokines and chemokines, more IgE production, and greater mucous cell metaplasia compared with wild-type mice. Adoptive transfer of house dust mite-pulsed dendritic cells from Vldlr-/- mice induced stronger airway inflammation in recipient mice after house dust mite challenge than wild-type dendritic cells. The VLDLR negatively regulates dendritic cell function and house dust mite-induced allergic airway
This document summarizes a study identifying the genetic cause of Heimler syndrome (HS). Whole exome sequencing was performed on individuals from eight families with HS. Biallelic mutations in the peroxisome biogenesis genes PEX1 or PEX6 were identified in six families. These findings define HS as a mild peroxisome biogenesis disorder caused by hypomorphic PEX1 or PEX6 mutations, expanding the clinical spectrum of these genes.
Jose Maria Morales - Spain - Tuesday 29 - HLA for Renal Allocationincucai_isodp
1. The document discusses the benefits of HLA matching in renal transplantation and how HLA matching is essential for retransplantation and sensitized patients.
2. It presents data showing improved graft and patient survival with better HLA matching and with newer immunosuppressive drugs.
3. The use of older donors and recipients is discussed, along with strategies like "old for old" transplantation, to better utilize available donor organs while maintaining patient outcomes.
Genetic variation and evolution and their importance to medicineDavid Enoma
Genetic variation is the driving force of evolution and is important in medicine. Single nucleotide polymorphisms are the most common genetic variation and can influence disease risk and drug responses between individuals and populations. Understanding genetic variation through studies of populations and single genes can provide insights into human evolutionary history, disease susceptibility, and treatment effectiveness.
Bill Faloon on Anti-Aging Drugs at DaVinci 50 Conference, 2021maximuspeto
In this presentation, Bill Faloon gives an update on the prospects of anti-aging drugs at the DaVinci 50 Masters Conference in Key Largo, Florida on April 29th, 2021.
This curriculum vitae summarizes the professional experience and qualifications of Michael John Dewey. It lists his education, including a Ph.D. in Microbiology from the University of Pennsylvania in 1973. It details his professional roles, such as Instructor at Colorado State University since 2007 and various director positions. It also provides a selection of his 75 publications in peer-reviewed journals on topics related to genetics and microbiology research.
La disponibilidad de un sistema de multiplicación del virus de la hepatitis C (VHC) infeccioso en cultivos celulares está permitiendo investigar nuevos factores de respuesta a tratamientos antivíricos en condiciones controladas. Se presentará evidencia de que el fitness vírico puede ser un factor de multiresistencia a inhibidores y quese pueden obtener eficientes reducciones de carga viral empleando diseños secuenciales de administración de inhibidores que incluyan ribavirina. Se discutirán posibilidades de aplicación clínica.
This document provides biographical and professional information about Dr. Subbarao Myla. It includes his contact information, research experience as Medical Director at Hoag Memorial Hospital Presbyterian Cardiology, publication highlights including 19 journal publications, and statistics on his research impact and readership. Dr. Myla specializes in interventional cardiology and has extensive experience in carotid artery stenting procedures.
This document summarizes a review article on the genetic, autoimmune, and environmental factors involved in rheumatoid arthritis (RA). It discusses how RA results from an interplay between these factors. Genetically, the HLA-DRB1 gene is a major determinant of RA risk. Over 30 non-MHC genes have also been associated with RA through genome-wide association studies and studies of specific populations, including STAT4, PADI4, and PTPN22. Environmental risks like smoking may interact with genetic susceptibility to increase RA risk. Understanding the roles of disease-associated genes and gene-environment interactions could lead to improved RA treatments and prevention strategies.
Transplantation involves transferring organs, tissues or cells from one part of the body to another or between individuals. Compatibility of immune molecules like HLA antigens, ABO blood groups, MIC antigens and KIR determines transplant success. Major histocompatibility complex (MHC) molecules control immune response and are targets in transplant rejection. Incompatibility can lead to hyperacute, acute cellular or chronic rejection as well as graft-versus-host disease. Immunosuppressive agents like corticosteroids, calcineurin inhibitors and monoclonal antibodies are used to suppress anti-graft immune responses.
This summary provides an overview of a study examining the use of intravenous immunoglobulin (IVIG) and plasmapheresis (PP) to treat acute humoral rejection (AHR) in kidney transplant recipients. The study analyzed 519 kidney transplant recipients between 1999-2003, identifying 23 patients (4.5%) with AHR. Most AHR patients were treated with IVIG and PP. Two-year graft survival for AHR patients treated with IVIG and PP (78%) was similar to those with acute cellular rejection (85%), and markedly better than historical rates without this treatment. The results suggest IVIG combined with PP is an effective treatment for AHR.
Bill Faloon gives update about human age-reversal clinical studiesmaximuspeto
In this presentation, Bill Faloon gives an update on clinical studies aimed and reversing age-related degeneration in humans at the DaVinci 50 Masters Conference in Key Largo, Florida on April 29th, 2021.
The document discusses organ transplantation, including the classification of graft rejection, pros and cons of transplantation, donor matching systems, examples of tissue transplantation, and how the success of a transplant depends on properly matching donors and recipients to avoid immune rejection. It also notes some of the major ethical issues surrounding transplantation relating to the source and methods of obtaining organs and distributive justice.
This document provides an overview of transplantation immunology. It discusses the different types of transplants including autografts, allografts, xenografts, and ABO incompatible transplants. It describes how the immune system can reject transplants and the challenges of finding donor-recipient matches. Key concepts covered include acute rejection occurring within 6 months, chronic rejection developing over longer periods, and the use of immunosuppressive drugs to reduce rejection risks. HLA tissue typing aims to find immunologically compatible donors by matching proteins on white blood cells.
Transplantation involves replacing an organ or tissue from a non-self donor with a recipient's own. Transplantation immunology studies the immune response against transplanted tissues that are histoincompatible. Rejection can involve cell-mediated immunity or antibody responses. Transplantation is an effective treatment for organ failure but rejection remains a challenge addressed through immunosuppressive drugs, though better approaches are needed to prevent rejection and reduce drug side effects while improving long-term outcomes.
This document lists 16 publications by Peter M. Power related to research on pilin glycosylation in Neisseria meningitidis and other gram-negative bacteria. The publications span from 2000 to 2014 and include studies characterizing the genetic basis of pilin glycosylation, the role of specific genes in the glycosylation pathway, comparative analyses of glycosylation mechanisms between bacteria, and investigations into genome diversity and phase variation.
This document compares the allele frequencies of 15 Plasmodium falciparum merozoite antigen genes in malaria infections sampled in Kenya in 2007 and 2008. It finds fluctuating allele frequencies in codons 147 and 148 of the reticulocyte-binding homologue 5 (Rh5) gene over this period in uncomplicated malaria infections. However, the dominant YH haplotype was stable over multiple years in asymptomatic and complicated infections. A regression analysis found the chance of the less common HD haplotype decreased over time from 2007 to 2009 in uncomplicated and asymptomatic infections.
This curriculum vitae summarizes the educational and professional experience of Peilin Ma, MD, PhD. Dr. Ma is currently an Assistant Research Professor at Indiana University School of Medicine, where she has worked since 2014 characterizing the molecular mechanisms underlying acute myeloid leukemia. She received her PhD in Immunology from Harbin Medical University in 2004 and MD in Clinical Medicine in 1997. Her research has focused on defining the roles of genes such as HoxA9, FLT3, C/EBPα, and Stat5 in leukemia development and identifying potential drug targets for treatment.
Robert Pesich_PAVA_Stanford Resume v. 8_22_16Robert Pesich
Robert Pesich has extensive experience managing laboratory operations and research projects. He has overseen the daily activities of 25 researchers at Stanford University and the Palo Alto VA, including managing budgets, equipment, and regulatory compliance. Pesich has specialized skills in tissue sample processing, gene expression analysis, and bioinformatics. He has authored several publications characterizing gene expression profiles in normal and diseased tissues. Currently, Pesich also serves as President of a poetry non-profit organization.
This document summarizes research investigating the role of the transcription factor Irx3 in regulating angiogenesis. The key findings are:
1) Irx3 expression is elevated in human microvascular endothelial cells in response to VEGF stimulation.
2) Genetic manipulation experiments showed that Irx3 promotes endothelial cell migration, chemotaxis, invasion, and tubulogenesis.
3) Irx3 overexpression increased the expression of Dll4, suggesting an increase in endothelial tip cell population.
4) Knockdown experiments identified Hey1, a downstream mediator of Notch signaling, as a negative regulator of Irx3 expression in response to VEGF.
So in summary, this research identifies Irx
This document discusses organ transplantation. It defines different types of transplants including autograft, allograft, isograft, and xenograft. It describes the most common transplants as kidney, heart, and liver transplants. For red blood cell transplants, the success is due to RBCs not expressing MHC antigens. It also discusses immune responses after transplantation, including acute and chronic rejection, and the use of immunosuppressive drugs for induction, maintenance, and specific therapy to reduce rejection.
The VLDLR attenuates house dust mite-induced airway inflammation by suppressing dendritic cell-mediated adaptive immune responses. Vldlr-/- mice challenged with house dust mite had increased eosinophilic and lymphocytic airway inflammation, higher Th2 cytokines and chemokines, more IgE production, and greater mucous cell metaplasia compared with wild-type mice. Adoptive transfer of house dust mite-pulsed dendritic cells from Vldlr-/- mice induced stronger airway inflammation in recipient mice after house dust mite challenge than wild-type dendritic cells. The VLDLR negatively regulates dendritic cell function and house dust mite-induced allergic airway
This document summarizes a study identifying the genetic cause of Heimler syndrome (HS). Whole exome sequencing was performed on individuals from eight families with HS. Biallelic mutations in the peroxisome biogenesis genes PEX1 or PEX6 were identified in six families. These findings define HS as a mild peroxisome biogenesis disorder caused by hypomorphic PEX1 or PEX6 mutations, expanding the clinical spectrum of these genes.
Jose Maria Morales - Spain - Tuesday 29 - HLA for Renal Allocationincucai_isodp
1. The document discusses the benefits of HLA matching in renal transplantation and how HLA matching is essential for retransplantation and sensitized patients.
2. It presents data showing improved graft and patient survival with better HLA matching and with newer immunosuppressive drugs.
3. The use of older donors and recipients is discussed, along with strategies like "old for old" transplantation, to better utilize available donor organs while maintaining patient outcomes.
Genetic variation and evolution and their importance to medicineDavid Enoma
Genetic variation is the driving force of evolution and is important in medicine. Single nucleotide polymorphisms are the most common genetic variation and can influence disease risk and drug responses between individuals and populations. Understanding genetic variation through studies of populations and single genes can provide insights into human evolutionary history, disease susceptibility, and treatment effectiveness.
Bill Faloon on Anti-Aging Drugs at DaVinci 50 Conference, 2021maximuspeto
In this presentation, Bill Faloon gives an update on the prospects of anti-aging drugs at the DaVinci 50 Masters Conference in Key Largo, Florida on April 29th, 2021.
This curriculum vitae summarizes the professional experience and qualifications of Michael John Dewey. It lists his education, including a Ph.D. in Microbiology from the University of Pennsylvania in 1973. It details his professional roles, such as Instructor at Colorado State University since 2007 and various director positions. It also provides a selection of his 75 publications in peer-reviewed journals on topics related to genetics and microbiology research.
La disponibilidad de un sistema de multiplicación del virus de la hepatitis C (VHC) infeccioso en cultivos celulares está permitiendo investigar nuevos factores de respuesta a tratamientos antivíricos en condiciones controladas. Se presentará evidencia de que el fitness vírico puede ser un factor de multiresistencia a inhibidores y quese pueden obtener eficientes reducciones de carga viral empleando diseños secuenciales de administración de inhibidores que incluyan ribavirina. Se discutirán posibilidades de aplicación clínica.
This document provides biographical and professional information about Dr. Subbarao Myla. It includes his contact information, research experience as Medical Director at Hoag Memorial Hospital Presbyterian Cardiology, publication highlights including 19 journal publications, and statistics on his research impact and readership. Dr. Myla specializes in interventional cardiology and has extensive experience in carotid artery stenting procedures.
This document summarizes a review article on the genetic, autoimmune, and environmental factors involved in rheumatoid arthritis (RA). It discusses how RA results from an interplay between these factors. Genetically, the HLA-DRB1 gene is a major determinant of RA risk. Over 30 non-MHC genes have also been associated with RA through genome-wide association studies and studies of specific populations, including STAT4, PADI4, and PTPN22. Environmental risks like smoking may interact with genetic susceptibility to increase RA risk. Understanding the roles of disease-associated genes and gene-environment interactions could lead to improved RA treatments and prevention strategies.
Transplantation involves transferring organs, tissues or cells from one part of the body to another or between individuals. Compatibility of immune molecules like HLA antigens, ABO blood groups, MIC antigens and KIR determines transplant success. Major histocompatibility complex (MHC) molecules control immune response and are targets in transplant rejection. Incompatibility can lead to hyperacute, acute cellular or chronic rejection as well as graft-versus-host disease. Immunosuppressive agents like corticosteroids, calcineurin inhibitors and monoclonal antibodies are used to suppress anti-graft immune responses.
This summary provides an overview of a study examining the use of intravenous immunoglobulin (IVIG) and plasmapheresis (PP) to treat acute humoral rejection (AHR) in kidney transplant recipients. The study analyzed 519 kidney transplant recipients between 1999-2003, identifying 23 patients (4.5%) with AHR. Most AHR patients were treated with IVIG and PP. Two-year graft survival for AHR patients treated with IVIG and PP (78%) was similar to those with acute cellular rejection (85%), and markedly better than historical rates without this treatment. The results suggest IVIG combined with PP is an effective treatment for AHR.
Bill Faloon gives update about human age-reversal clinical studiesmaximuspeto
In this presentation, Bill Faloon gives an update on clinical studies aimed and reversing age-related degeneration in humans at the DaVinci 50 Masters Conference in Key Largo, Florida on April 29th, 2021.
The document discusses organ transplantation, including the classification of graft rejection, pros and cons of transplantation, donor matching systems, examples of tissue transplantation, and how the success of a transplant depends on properly matching donors and recipients to avoid immune rejection. It also notes some of the major ethical issues surrounding transplantation relating to the source and methods of obtaining organs and distributive justice.
This document provides an overview of transplantation immunology. It discusses the different types of transplants including autografts, allografts, xenografts, and ABO incompatible transplants. It describes how the immune system can reject transplants and the challenges of finding donor-recipient matches. Key concepts covered include acute rejection occurring within 6 months, chronic rejection developing over longer periods, and the use of immunosuppressive drugs to reduce rejection risks. HLA tissue typing aims to find immunologically compatible donors by matching proteins on white blood cells.
Transplantation involves replacing an organ or tissue from a non-self donor with a recipient's own. Transplantation immunology studies the immune response against transplanted tissues that are histoincompatible. Rejection can involve cell-mediated immunity or antibody responses. Transplantation is an effective treatment for organ failure but rejection remains a challenge addressed through immunosuppressive drugs, though better approaches are needed to prevent rejection and reduce drug side effects while improving long-term outcomes.
This document lists 16 publications by Peter M. Power related to research on pilin glycosylation in Neisseria meningitidis and other gram-negative bacteria. The publications span from 2000 to 2014 and include studies characterizing the genetic basis of pilin glycosylation, the role of specific genes in the glycosylation pathway, comparative analyses of glycosylation mechanisms between bacteria, and investigations into genome diversity and phase variation.
This document compares the allele frequencies of 15 Plasmodium falciparum merozoite antigen genes in malaria infections sampled in Kenya in 2007 and 2008. It finds fluctuating allele frequencies in codons 147 and 148 of the reticulocyte-binding homologue 5 (Rh5) gene over this period in uncomplicated malaria infections. However, the dominant YH haplotype was stable over multiple years in asymptomatic and complicated infections. A regression analysis found the chance of the less common HD haplotype decreased over time from 2007 to 2009 in uncomplicated and asymptomatic infections.
This curriculum vitae summarizes the educational and professional experience of Peilin Ma, MD, PhD. Dr. Ma is currently an Assistant Research Professor at Indiana University School of Medicine, where she has worked since 2014 characterizing the molecular mechanisms underlying acute myeloid leukemia. She received her PhD in Immunology from Harbin Medical University in 2004 and MD in Clinical Medicine in 1997. Her research has focused on defining the roles of genes such as HoxA9, FLT3, C/EBPα, and Stat5 in leukemia development and identifying potential drug targets for treatment.
Robert Pesich_PAVA_Stanford Resume v. 8_22_16Robert Pesich
Robert Pesich has extensive experience managing laboratory operations and research projects. He has overseen the daily activities of 25 researchers at Stanford University and the Palo Alto VA, including managing budgets, equipment, and regulatory compliance. Pesich has specialized skills in tissue sample processing, gene expression analysis, and bioinformatics. He has authored several publications characterizing gene expression profiles in normal and diseased tissues. Currently, Pesich also serves as President of a poetry non-profit organization.
This document provides a summary of Michael L. Avery's education and professional experience. He holds a BS and BGS in Cell Biology and Microbiology from the University of Kansas, and an MA in Microbiology. His experience includes over 13 years in analytical sciences and process development at Amgen, analytical chemistry research at Sigma-Aldrich Biosciences, and various roles supporting drug development and preclinical studies at 3M Pharmaceuticals and Quintiles. He has extensive experience in analytical techniques such as HPLC, CE, LC/MS, and related methods for characterizing proteins and small molecules.
Mohammad Rahman is a senior research associate at Ohio State University studying cancer metastasis. He has a PhD in cancer biology from Shimane Medical University in Japan and has held several postdoctoral research positions. His research focuses on how exosomes secreted by cancer cells drive metastasis by inducing epithelial to mesenchymal transition in other cells. He recently discovered that lung cancer cell exosomes can induce EMT in recipient cells, which may be important for establishing the tumor microenvironment and cancer spread. The goal of his current research proposal is to investigate the mechanisms by which cancer-derived exosomal contents drive metastasis.
Mohammad Rahman is a senior research associate at Ohio State University studying cancer metastasis. He has a PhD in cancer biology from Shimane Medical University in Japan and has held several postdoctoral research positions. His research focuses on how exosomes secreted by cancer cells drive metastasis by inducing epithelial to mesenchymal transition in other cells. He recently discovered that lung cancer cell exosomes can induce EMT in recipient cells, which may be important for establishing the tumor microenvironment and cancer spread. The goal of his current research proposal is to investigate the mechanisms by which cancer-derived exosomal contents drive metastasis.
This document is a CV for Dr. Martin Mugar Pike, who is seeking a challenging position utilizing his experience in disease biology and therapeutics. He has over 30 years of experience in areas such as cancer pathophysiology, cell metabolism, tumor angiogenesis, and medical imaging techniques. His professional experience includes research roles focusing on glioma therapeutics and stroke at Oregon Health & Science University and the University of Alabama at Birmingham. He has authored over 20 peer-reviewed publications and has a track record of collaborative research and leadership experiences.
This document outlines a research protocol to study neutrophil lymphocyte ratio (NLR) and platelet lymphocyte ratio (PLR) as predictors of systemic inflammation and chronic illness in pre-surgery patients. The study will retrospectively analyze data from 1000 patients who attended a preadmission clinic between January and April 2013. Medical history, exam findings, and blood test results will be collected to calculate NLR and PLR ratios and examine their relationship to inflammation and health conditions. The goal is to determine the prevalence of elevated ratios and their ability to predict surgical risk factors.
This document summarizes the qualifications and experience of Shumei Ren, a biomedical research investigator specializing in oncological diseases. Ren has over 15 years of experience in molecular biology, pharmacology, and various techniques including flow cytometry, in vitro assays, and high throughput methods. Ren's professional experience includes positions at Thomas Jefferson University, Albany Medical College, and Hokkaido University investigating topics such as gastric cancer, prostate cancer, fibrosis, and hematopoietic malignancies.
This curriculum vitae summarizes the educational and professional experience of Dr. Wen-Hai Chou. He received his PhD from the University of Texas Health Science Center in 2000 and is currently an Assistant Professor at Kent State University. His research focuses on the roles of Protein Kinase C and Lipocalin-2 in stroke. He has over 10 peer-reviewed publications in high impact journals such as the Journal of Neuroscience and Journal of Biological Chemistry.
This curriculum vitae outlines the education and work experience of Aleksandra Stojanovic-Terpo. She received BS and MS degrees in biochemistry and biotechnology from the University of Belgrade in 1999. She has worked as a research specialist at the University of Illinois at Chicago since 2004, investigating platelet activation pathways. She has authored or co-authored over 15 peer-reviewed publications and presented her research at several conferences.
Identification of a common Wnt-associated genetic signature across multiple c...Rubin Baskir, Ph.D.
1. The study identified a common Wnt-associated genetic signature across multiple cell types derived from patients with pulmonary arterial hypertension (PAH), including mesenchymal stromal cells, endothelial cells, and fibroblasts.
2. Gene expression profiling of induced pluripotent stem cell-derived mesenchymal cells and endothelial-like cells from PAH patients found deregulation of the Wnt signaling pathway. This was validated in primary patient mesenchymal and endothelial cells.
3. Analysis of fibroblasts from patients with heritable and idiopathic PAH also identified alterations in Wnt pathway genes. This suggests that molecular defects causing PAH are present in all cell types studied, regardless of origin, and involve
This document is a resume for Sandra Anderson LaSalle, who has over 20 years of experience in clinical and basic science research. She has worked in a variety of roles including as a research scientist at Merck, Pfizer, and various universities. Her experience includes laboratory management, product development, clinical research, and teaching. She has advanced knowledge in microbiology and experience in fields like oncology, diabetes, and female reproduction.
This document provides a summary of Patricia Grutkoski's professional experience and qualifications. She has over 20 years of experience in research and diagnostic laboratories, including launching laboratory developed tests and implementing new FDA-approved assays. Her areas of expertise include clinical studies using animal and human subjects under various regulatory guidelines. She has a Ph.D. in molecular biology and has held several leadership roles supervising staff and projects at various research institutions and companies.
This document provides a summary of Aaron M Bender's background and experience. It includes his contact information, educational background which includes a PhD in Molecular Biology from the University of Wyoming, and extensive research experience including positions at ArcherDX, the University of Kansas Molecular Probes Core Laboratory, the University of Kansas, and the Mayo Clinic where he conducted research in areas such as cancer genetics, chemical biology, next generation sequencing, and the use of model organisms like C. elegans. He has over 15 publications in peer-reviewed journals.
Vicky M.-H. Sung has over 20 years of experience in biomedical research. She received her Ph.D. in Molecular Microbiology and Immunology from the University of Southern California. Her resume summarizes her educational background and work experience conducting independent research projects at prestigious institutions such as Harvard Medical School, the University of California Irvine, and the University of Southern California. She has extensive expertise in areas such as viral infections, stem cell biology, and molecular biology techniques.
Comparative analysis of gene expression reulation in mouse rat and human Spri...constantina mylona
This document discusses animal models and their importance in biomedical research. It focuses on mice and rats as the most widely used animal models. Mice and rats are useful models because they share many physiological similarities with humans, have short lifecycles, and their genomes share a high percentage of DNA similarity. The document also introduces CRISPR/Cas9 as a tool for generating transgenic and gene-edited rodent models. It aims to validate whether the mouse is the most suitable model by comparing gene expression regulation between human, mouse and rat.
Lisa Salvador is a pharmaceutical research professional with over 10 years of industry experience focusing on clinical biomarkers, translational medicine, and drug development. She has a PhD in Cell Biology from Northwestern University and has held senior research roles at Bristol-Myers Squibb and GlaxoSmithKline. Her expertise includes cellular and molecular biology, gene therapy research, and diagnostic assay development. She has received numerous awards for her research contributions and leadership.
This curriculum vitae summarizes Michelle Renee Tourigny's work experience and education. She has over 20 years of experience in flow cytometry and 14 years working with mouse models. Her positions include graduate research assistant, flow cytometry facility manager, and postdoctoral fellow. She received a Ph.D. in Immunology from Cornell University and has skills in flow cytometry, cell culture, mouse work, and immunological techniques.
This document is a resume for Christine M. Kornmeier, who has over 30 years of experience in research and development roles in the life sciences and drug discovery fields. She has worked at several large pharmaceutical companies, managing multiple projects involving assay development, high-throughput screening, and developing inhibitors for various disease targets. She also has experience mentoring interns and training sales teams.
This curriculum vitae outlines Curtis T. Thompson's professional experience and education. He is currently a dermatopathologist in private practice in Portland, Oregon and an affiliate associate professor at Oregon Health & Science University. He has over 20 years of experience in dermatopathology research, teaching, and consulting. He is involved in several current research projects focused on improving detection of fungi and characterizing inflammatory cells in skin diseases.
1. Christine Roos Montague
186 Buck Rd Phone: 607-379-1151
Lansing, NY 14882 Email: cmontague70@gmail.com
EDUCATION
University of Virginia, Charlottesville, VA
Ph.D. in Biomedical Engineering, 1995, with high distinction
Chronic inhaled nitric oxide: effects on pulmonary vascular endothelial function and pathology in
hypoxic and normoxic rats.
University of Iowa, Iowa City, IA
M.S. in Biomedical Engineering, 1990
University of Virginia, Charlottesville, VA
B.S. in Mechanical Engineering, 1986
PROFESSIONAL EXPERIENCE
Research Scientist, Agave Biosystems, Ithaca, NY, May 2010-Feb 2015.
Characterized for a published manuscript the genomic and proteomic responses to two small compounds
identified in high-throughput screening as improving mitochondrial function.
Optimized high-throughput assays for the identification of agonists or antagonists of the αKlotho and βKlotho-
dependent endocrine Fibroblast Growth Factors.
Engineered and freeze-dried bacterial and yeast sensors containing toxin-responsive reporter constructs.
Managed two projects. Communicated the results through periodic reports and presentations.
Wrote multiple grant proposals including human and animal subjects testing sections: an isothermal
amplification technique to simultaneously detect all five hepatitis viruses in a point-of-care device, a multiplex
assay for arthritis biomarkers, and an amplification device to detect Salmonella in barn waste.
Research Associate, Cornell University School of Engineering, Department of Biomedical
Engineering, Ithaca, NY, July 2008-May 2010, Cynthia Reinhart-King, PI.
Studied endothelial dysfunction caused by matrix stiffening.
Postdoctoral Associate, Cornell University School of Veterinary Medicine, Clinical Sciences, Ithaca,
NY, July 2006-2008, Lisa A. Fortier, PI.
Examined the effects of fibronectin fragments on cartilage cells.
Research Associate, Ohio State University School of Medicine, Heart and Lung Research Institute,
Columbus, OH, 1997-2006, Pascal J. Goldschmidt-Clermont and Clay B. Marsh, PIs.
Characterized estrogen receptor activation in vascular cells and its role in heart disease manifestation in
women.
Postdoctoral Fellow, Johns Hopkins University School of Medicine, Pulmonary and Critical Care
Medicine, Baltimore, MD, 1995-1997, Nicholas A. Flavahan and Pascal J. Goldschmidt-Clermont, PIs.
Began studies of hormonal and vascular interactions in Cardiology and Pulmonary divisions.
Graduate Research Fellow, University of Virginia Department of Anesthesiology, Charlottesville, VA,
1991-1995, George F. Rich, PI.
Characterized the responses to inhaled nitric oxide in pulmonary vasculature of rats exposed to chronic
hypoxia.
Teaching Assistant, University of Virginia Department of Mechanical Engineering, Charlottesville, VA,
1991, Thermodynamics.
Ran tutorials and office hours for thermodynamics.
2. Clinical Research Assistant, Diabetes Research Institute, Norfolk, VA, summer 1990, Aaron I. Vinik.
Performed EKG and neuropathy testing on clinic patients, introduced and developed fractal analysis of EKG
signal to determine extent of illness.
Mechanical Engineer, Norfolk Naval Shipyard, Portsmouth, VA, 1986-1989.
Designed installations for upgrades in steam and water systems in nuclear cruisers.
HONORS
1990 The Hand Foundation Graduate Fellowship Award
1992 Three-year graduate fellowship from the Department of Anesthesiology, University of
Virginia School of Medicine
1992 Tau Beta Pi, Engineering Honor Society
1995 Graduate of High Distinction, University of Virginia
1996-1998 NRSA Postdoctoral Fellowship, NHLBI, National Institute of Health (Hormonal
Regulation of Dysfunctional Endothelial Cells)
1991-2001 AHA Ohio Affiliate Postdoctoral Fellowship Award (Enhanced Binding to Fibrinogen of
Glycoprotein IIb-IIIa Receptors with the PlA2 Polymorphism)
RESEARCH SKILLS
Cell biology:
Primary cell culture, fluorescent microscopy, and transfection.
Flow cytometry for immunofluorescence, apoptosis assays and BrdU incorporation.
Molecular biology:
Lentiviral plasmid design, cloning, virus production and transduction.
Quantitative PCR and design of Taqman primer/probe sets.
Protein, RNA and DNA purification, blotting and detection. Endotoxin testing.
Activation domain or immunocomplex precipitation.
siRNA design for oligo or plasmid transfection or Lentiviral transduction.
Bioinformatics: analysis of microarray data, eukaryotic and prokaryotic genomes
Assay Development:
High-throughput experimental design, automation.
Reporter construct cloning, transformation and analysis.
PCR and isothermal amplification assay design.
ELISA development.
Statistical analysis.
Other Skills:
Project management and communication with collaborators and sponsors.
Publication and grant proposal writing, including human and animal research protocols.
Training, laboratory organization, budgeting, purchasing and scheduling.
PROFESSIONAL MEMBERSHIPS
American Society for Cell Biology
American Heart Association
PUBLICATIONS
1. Montague CR, Fitzmaurice A, Hover BM, Salazar NA, Fey JP. Screen for Small Molecules Increasing the
Mitochondrial Membrane Potential. 2014. J Biomol Screen 19(3):387-98.
2. Huynh J, Nishimura N, Rana K, Peloquin JM, Califano JP, Montague CR, Schaffer CB, King MR, and
Reinhart-King CA. Age-Related Intimal Stiffening Promotes Atherosclerosis by Enhancing Endothelial
Permeability and Leukocyte Transmigration. 2011. Science Translational Medicine 3:112ra122.
3. Ezzie ME, Piper MG, Montague C, Newland CA, Opalek JM, Baran C, Ali N, Brigstock D, Lawler J, Marsh
CB. Thrombospondin-1 Deficient Mice Are Not Protected from Bleomycin-Induced Pulmonary Fibrosis.
2011. American Journal of Respiratory Cell and Molecular Biology 44:556-61.
3. 4. Montague CR, Hunter MG, Gavrilin M, Goldschmidt-Clermont PJ, Marsh CB. Activation of estrogen
receptor-alpha reduces aortic smooth muscle differentiation. 2006. Circulation Research 99:477-484.
Prompted an editorial (Pepine CJ, Nichols WW. Estrogen and Different Aspects of Vascular Disease in
Women and Men. Circulation Research 99:459-461)
5. Lichtenberger FJ, Montague CR, Hunter M, Frambach GE, Marsh CB. NAC and DTT promote TGF-1
monomer formation: demonstration of competitive binding. 2006. Journal of Inflammation; 3:7.
6. Boudoulas KD, Montague CR, Goldschmidt-Clermont PJ, Cooke GE. Physiological Estradiol Increases
Aggregation of PlA1/A1
Platelets. 2006. Am Heart J 152:136-9.
7. Chotani MA, Mitra S, Su BY, Flavahan S, Eid AH, Clark KR, Montague CR, Paris H, Handy DE, Flavahan
NA. Regulation of 2-adrenoceptors in human vascular smooth muscle cells. 2004. American Journal of
Physiology: Heart Circulatory Physiology 286:H59-67.
8. Eubank TD, Galloway M, Montague CM, Waldman WJ, Marsh CB. M-CSF induces vascular endothelial
growth factor production and angiogenic activity from human monocytes. 2003. Journal of Immunology
171:2637-43.
9. Boudoulas KD, Cooke GE, Roos CM, Bray PF, Goldschmidt-Clermont PJ. The PlA2
polymorphism of
glycoprotein IIIa functions as a modifier for the effect of estrogen on platelet aggregation. 2001. Archives of
Pathology and Laboratory Medicine 125:112-115.
10. Vijayan KV, Goldschmidt-Clermont PJ, Roos C, Bray PF. The PlA2
polymorphism of integrin 3 enhances
outside-in signaling and adhesive functions. 2000. Journal of Clinical Investigation 105:793-802.
11. Ying AK, Hassanain HH, Roos CM, Smiraglia DJ, Issa JJ, Michler RE, Caligiuri M, Plass C, Goldschmidt-
Clermont PJ. Methylation of the estrogen receptor-alpha gene promoter is selectively increased in
proliferating human aortic smooth muscle cells. 2000. Cardiovascular Research 46:172-179.
12. Goldschmidt-Clermont PJ, Roos CM, Cooke GE. Platelet PlA2
polymorphism and thromboembolic events:
from inherited risk to pharmacogenetics. 1999. Journal of Thrombosis and Thrombolysis 8:89-103.
13. Irani K, Pham Y, Coleman L, Roos C, Cooke G, Miodovnik A, Karim N, Wilhide C, Bray P, Goldschmidt-
Clermont P. Priming of platelet IIb3 by oxidants is associated with tyrosine phosphorylation of 3. 1998.
Arteriosclerosis Thrombosis and Vascular Biology 18:1698-1706.
14. Frank DU, Lowson SM, Roos CM, Rich GF. Endotoxin alters hypoxic pulmonary vasoconstriction in
isolated rat lungs. 1996. Journal of Applied Physiology 81:1316-1322.
15. Uncles DR, Daugherty MO, Frank DU, Roos CM, Rich GF. Nitric oxide modulation of pulmonary vascular
resistance is red blood cell dependent in isolated rat lungs. 1996. Anesthesia and Analgesia 83:1212-1217.
16. Roos CM, Frank DU, Xue C, Johns RA, Rich GF. Chronic inhaled nitric oxide: effects on pulmonary
vascular endothelial function and pathology in hypoxic and normoxic rats. 1996. Journal of Applied
Physiology 80:252-260.
17. Anderson SM, Rich GF, Roos CM, Lee LP, Lee JS. Fluid restitution and blood volume change in
anesthetized rabbits in response to vasoactive drugs. 1994. Circulation 90:509-514.
18. Roos CM, Rich GF, Uncles DR, Daugherty MO, Frank DU. Sites of vasodilation by inhaled nitric oxide
versus sodium nitroprusside in endothelin constricted isolated rat lungs. 1994. Journal of Applied
Physiology 77:51-57.
19. Rich GF, Roos CM, Anderson SM, Daugherty MO, Uncles DR. Direct effects of intravenous anesthetics on
pulmonary vascular resistance in the isolated rat lung. 1994. Anesthesia and Analgesia 78:961-966.
20. Rich GF, Murphy GD, Roos CM, Johns RA. Inhaled nitric oxide: selective pulmonary vasodilation in cardiac
surgical patients. 1993. Anesthesiology 78:1028-1035.
21. Rich GF, Roos CM, Anderson SM, Urich DC, Daugherty MO, Johns RA. Inhaled nitric oxide: dose
response and the effects of blood in the isolated rat lung. 1993. Journal of Applied Physiology 75:1278-
1284.