Btm4 histology
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This document discusses various microscopic techniques used to study the effects of orthodontic forces on tissues, including histology, histochemistry, immunohistochemistry, and autoradiography. It provides examples of prominent orthodontic researchers who used these techniques, such as Kaare Reitan who studied how factors like force duration affect tissue remodeling. Specific techniques can identify enzyme activity and localization, as well as molecules involved in force-induced tissue changes. Microscopy overall reveals the morphological and functional responses of tissues to orthodontic treatment on a cellular and molecular level.
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Btm4 histology
- 1. Histological, histochemical, and immunohistochemical investigations into the effects of orthodontic forces on paradental tissues and cells www.indiandentalacademy.com
- 2. Histology The branch of biology concerned with the microscopic study of the structure of tissues The tissue structure of an organism or part, as revealed by a microscopic study (Webster’s Dictionary) www.indiandentalacademy.com
- 4. Prominent orthodontic histologists Carl Sandstedt (1904-5): apposition and resorption of alveolar bone, mainly at the PDL-bone surface interface Albin Oppenheim(1911-2): complete transformation of the alveolar process Kaare Reitan (1947-70s): effects of variables such as age, gender, species, type and duration of force application www.indiandentalacademy.com
- 29. 3 hours tipping, 80 g www.indiandentalacademy.com
- 52. Summary of clinical implications of tooth movement-related histological observations (Kaare Reitan and Per Rygh) www.indiandentalacademy.com
- 67. Histochemistry That branch of histology which deals with the identification of chemical components in cells and tissues (Medical Dictionary). The study of the chemical components of cells through the use of chemically specific staining reagents (Webster). www.indiandentalacademy.com
- 68. Enzymes with enhanced activity during orthodontic PDL compression in man (E. Lilja et al, 1983) Lactate dehydrogenase Glucose-6-phosphate dehydrogenase Succinic dehydrogenase Acid phosphatase Aminopeptidase M Aryl sulfatase Prostaglandin synthetase www.indiandentalacademy.com
- 69. Localization of enzymes in the compressed PDL in rats (E Lilja, S Lindskog, L Hammarstrom, 1983) Mesial molar movement in rats for 10 h-6 d RESULTS: acid phosphatase and lactate dehydrogenase were found in sites of alveolar bone resorption. Prostaglandin synthetase was found exclusively in the bone marrow, but not in the PDL. www.indiandentalacademy.com
- 74. Conclusions from orthodontic- related histochemical studies Specific enzymes can be localized and identified in mechanically-stressed paradental cells. Cellular enzymatic activity is enhanced by orthodontic forces. Monitoring enzymatic fluctuations in the gingival crevicular fluids may have diagnostic value. www.indiandentalacademy.com
- 75. Immunohistochemistry Microscopic localization of molecular components of tissues through the use of antibodies against these molecules. www.indiandentalacademy.com
- 87. Effect of orthodontic tension on 3’,5’-adenosine monophosphate (cyclic AMP, cAMP) in cat canine paradental cells www.indiandentalacademy.com
- 92. Immunohistochemistry in orthodontic research It can determine the presence, location, and relative concentration of specific molecules in dental and paradental tissues during tooth movement. This information can assist in identifying molecules that play important roles in force-induced tissue remodeling. www.indiandentalacademy.com
- 93. Autoradiography The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by weakly emitting radioactive material within the object. The technique has been used widely for studying DNA synthesis and location within cells, with tritium-labeled precursors (like thymidine) as the radioactive marker. (Medical Dictionary) www.indiandentalacademy.com
- 103. Conclusion: autoradiography and orthodontics Autoradiography facilitates the determination of the origin of cells that participate in the remodeling of paradental tissues during orthodontic treatment. It also enables to assess the location and amount of newly synthesized ECM, and the extent of blood flow through the involved tissues. www.indiandentalacademy.com
- 104. Overall conclusions Microscopy in its various modes is a powerful tool to expose the effects of orthodontic forces on paradental tissues. It is useful in highlighting morphological and functional changes, on the cellular and molecular levels. It provides the orthodontist means to assess the outcome of various treatment modalities, and a scientific basis for choosing specific techniques. www.indiandentalacademy.com
Editor's Notes
- 3 hours tipping, 80 g
- 7