This document describes the case of a sick 5-year-old female buffalo in its 7th month of pregnancy. The buffalo presented with inappetence, head pressing, bilateral lacrimation, debility, swollen hind limbs, difficulty bearing weight, and occasional circling. Bloodwork showed microcytic anemia and microfilaria larvae were found on microscopy. Fecal analysis detected Paramphistomes eggs. The buffalo was treated with oxytetracycline, anti-inflammatories, vitamins, and dextrose saline initially with no improvement. Further testing at VHRTC found microfilaria with nuclei on Geimsa's stain. Treatment with ivermectin cured the buffalo within

1. Dr.jibachha Sah
M.D.
VHRTC(P)LTD,LANKU,NGT,CHITWAN

2.  Inappitance
 Head pressing
 Bilateral lacrimated
 Debilated
 Swollen hind limb
 Some times circulling
 Unable to bear weight from hind limb.

3. Clinically sick buffalo

4. Animal
species
Buffalo Age 5th
Lactation
Sex Female
Pregnancy 7 th
month
Breed Mx

5. 1.The owner first notices buffaloed inappetance and debilated about on and a half month.
2.Buffaloed was seen by veterinary technician for this problem about 3 weeks ago. The
veterinary technician did treatment based on clinical sign and symptoms.
3.Buffaloe was head pressing towards the wall of shed.
4.Swollen of hind limb and unable to bear weight also.
5.Before starting therapy, the owners report that buffalo was eating continuously, and after
treatment usually laying down would like to sleeping
6.The owners reports that buffaloed gets short of breath when he walk, but also sometimes
just laying down.
7.Buffalo energy level has-been much lower than usual since this problem began.

6. 1.Temperature 103.2,Pulse NA,Respiratory Rate NA.
2.Bilateral lacrimation.
3.There is unilateral swollen of hind limb joint. He has great difficulty moving
seems pain full in right sided limbs.
4.Inspiratory and expiratory breath sounds are normal.
5.There is no swollen of prescapular lymphnodes.
6.Rumen motility was also normal.
7.Owner reported that urination and feces is normal, She is 7 th month pregnant
so milking was dried off.

7.  The buffalo was treated by local veterinary technician with
 Oxytetra cycline Long acting @ 40 ml. i/m .
 Proxy vet inj. @20 ml i/m (Anti-inflammatory and antipyretic)
 Dextrose saline @2 bottle i/v x 2 days
 Neurovitamin (Vit.B1,B6,& B12 inj.)@10ml i/m x 3days.
 Rumen bolus (Ruminotorics bolus) @2 bolus b.i.dx5 days.
But Not Improvement
Than refer to VHRTC

8. Blood CBC
Urine R/E
Fecal test

9. Parameter Finding Normal Reference
Hb(g%) 8.0 8-15(11)
PCV (%) 24.0 24-46(35)
RBC(m/cumm) 4.0 5-10 ( 7.0)
WBC(thousand/cumm)
DLC count
Neutrophil % 42 15-45(28)
Lymphocyte % 55 45-75(58)
Eosinophil % 02 2-20(9)
Monocytes % 01 2-4(4)
MCV 60 40-60(52.0)
MCH 20 11-17(14.0)
MCHC 33.33 30-36(33.0)
Parasites
Microcytic anemia (higher
Microfilaria larvae were found in microscope slide

10. Parameter Finding
Colour Light yellow
Consistency Clear
PH 8.0
Sp.Gr. 1.003
Glucose ++
Protein nil
ketone nil
Bilurubin nil
Urobilinogen nil
Microscopic examination: Cast cell: 0/hpf
RBC: WBC : 0/hpf

11. Paramphistomes eggs positive

13. Microfileria with nuclei Geimsa’s stain 100x

14.  Ivermectin Injection (Kepromec Inj )1ml/50 kg
body wt.s/c once.

15. The buffalo was completely cure within 5 days.
Appetite and all the clinical symptoms were recovered
and not further treatment was required.