The document outlines a lesson plan for the B.Pharmacy course on Pharmaceutical Biotechnology, detailing its objectives, outcomes, and topics to be covered over 45 lectures. Key topics include immobilized enzymes, genetic engineering, monoclonal antibodies, and fermentation technology. It concludes with references to relevant academic resources and emphasizes the importance of practical applications in the pharmaceutical industry.

1. LESSON PLAN
Name of the Programme: B.Pharmacy
Course name: PHARMACEUTICAL BIOTECHNOLOGY (Theory)
Course code: BP 605 T
Faculty name: Dr. Sudipta Roy
Name of the class: Semester II Section A
Subject name: PHARMACEUTICAL BIOTECHNOLOGY (Theory)
Subject code: BP 605 T
Scope of the Subject/Course:
Upon completion of the course the student shall be able to:
1. Understanding the importance of Immobilized enzymes in Pharmaceutical
Industries
2. Genetic engineering applications in relation to production of pharmaceuticals
3. Importance of Monoclonal antibodies in Industries
4. Appreciate the use of microorganisms in fermentation technology
Course outcome: Upon the completion of the course student shall be able to
Unit-I Understanding the importance of Immobilized enzymes in
Pharmaceutical Industries.
Unit-II Genetic engineering applications in relation to production of
pharmaceuticals
Unit-III Importance of Monoclonal antibodies in Industries
Unit-IV Importance of Monoclonal antibodies in Industries
Unit-V Appreciate the use of microorganisms in fermentation technology

2. 1 | P a g e
Total Number of lecture-45
Name of the Topic Numbe
r of
classes
require
d
Cumulative
number of
periods/week
Actu
al
date
of
comp
le
tion
Teaching
learning
method/
Teaching Aid
Refere
nces
Signatur
e of
academic
head
1. Brief introduction to
Biotechnology with
reference to
Pharmaceutical Sciences
2. Enzyme Biotechnology-
Methods of enzyme
immobilization and
applications
3. Biosensors- Working
4. Applications of
biosensors in
Pharmaceutical
Industries
5. Brief introduction to
Protein Engineering
6. Use of microbes in
industry. Production of
Enzymes
7. General consideration -
Amylase, Catalase
8. Peroxidase, Lipase
9. Protease, Penicillinase
10. Basic principles of
genetic engineering
11. Study of cloning vectors
12. Restriction
endonucleases
13. DNA ligase
14. Recombinant DNA
technology
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
Chalk and talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and
Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3

3. 15. Application of genetic
engineering in medicine
16. Application of r DNA
technology
17. Genetic engineering in
the production
18. i) Interferon
19. ii) Vaccines- hepatitis-
B
20. iii) Hormones-Insulin
21. d) Brief introduction to
PCR
22. Types of immunity-
humoral immunity,
cellular immunity
23. a) Structure of
Immunoglobulins
24. b) Structure and
Function of MHC
25. c) Hypersensitivity
reactions, Immune
stimulation and Immune
suppressions.
26. d) General method of
the preparation of
bacterial vaccines,
27. Toxoids, viral vaccine,
antitoxins, serum-
immune blood
derivatives and other
products relative to
immunity.
28. e) Storage conditions
and stability of official
vaccines
29. f) Hybridoma
technology- Production,
Purification and
Applications
30. g) Blood products and
Plasma Substituties.
31. a) Immuno blotting
techniques- ELISA,
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,23
1,2,3
1,2,3

4. 32. Western blotting
33. Southern blotting.
34. b) Genetic organization
of Eukaryotes
35. Prokaryotes
36. c) Microbial genetics
including
transformation,
transduction,
37. Conjugation,
38. Plasmids and
transposons.
39. d) Introduction to
Microbial
biotransformation and
applications.
40. e) Mutation: Types of
mutation/mutants.
41. a) Fermentation
methods and general
requirements, study of
media, equipments
42. Sterilization methods,
aeration process, stirring
43. b) Large scale
production fermenter
design and its various
controls
44. c) Study of the
production of -
penicillins, citric acid
45. Vitamin B12, Glutamic
acid, Griseofulvin
46. d) Blood Products:
Collection, Processing
and Storage of whole
human blood, dried
47. Human plasma
48. Plasma Substituties
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
Chalk and Talk
Chalk and Talk
Chalk and Talk
Chalk and Talk
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
1,2,3
Reference:

5. 1. Industrial Biotechnology, Dr. N. N. Bandela, Nirali Prakashan, Pune
2. A text of Biotechnology, Dr. R C Dubey, S.Chand and Company limited,
New Delhi
3. Pharmaceutical Biotechnology, Prof. Chandrakanta Kokate, Nirali Prakasan, Pune
Teacher 1: Dr. Sudipta Roy
Signature of Principal
9