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M HussainTaqi
Abida ameen
Ayesha Amen
M Ramzan
Dilahad Makki
Amara bibi
+
+
+
Lethal Dose (LD50)
+ It is a dose/ amount of any chemical/virus suspension
that can kill 50% of the test population in a specific
time period.
Embryo infectious dose (EID50)
+ It is a dose or amount of virus suspension that
can infect 5 0 % of the test population in a
specific time period.
Tissue culture infectious dose (TCID50)
+ It is the dose of virus which can cause cytopathic
effect (CPE) in 50% of tissue culture cells in specific
time period.
Procedure
+ 10-fold serial dilution should be performed( 9ml PBS
suspension, 1ml viral sample)
+ Inoculation of dilutions into the cell.
+ After five days observe cytopathic effect (CPE).
+ Calculate the dose.
Methods for cultivation of virus
+ Viruses can be cultivated within suitable hosts,
such as a living cell. Generally three methods
are employed for the virus cultivation.
-
An embryonated egg offers various site of inoculation:
- Allantoic cavity
- Amniotic cavity
- Chorioallantioc membrane
- Yolk sac
Calculation by Read And Muench Method
Biological titration of microorganisms..docx
Biological titration of microorganisms..docx
Biological titration of microorganisms..docx

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