This document summarizes best practices in cycling from leading European cities and around the world. It describes policies that have increased bicycle usage and presents methods for evaluating cycling infrastructure and policies. The document provides indicators for benchmarking cities' cycling levels and suggestions for assessment methodologies. It aims to help increase cycling in the BICY project region in Central Europe. Key topics covered include cycling infrastructure design, legal and policy barriers, public transportation integration, education, and the diversity of bicycles and cyclists that need to be accommodated.