Sistema de liquidos

1. © 2014 Mindray Confidential
BC-5300(new)/5380(new) Service Training
Hematology Global Technical Support Department

2. © 2013 Mindray Confidential
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Contents
 Product Overview
 Counting Principle
 Hardware System
 Mechanical System
 Installation
 Software System
 Liquid System

3. © 2013 Mindray Confidential
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Counting Principle Review
 Diff Channel
Laser + FCM
 WBC/HGB Channel
Electrical Impedance Method
Colorimetric Method
 RBC/PLT channel
Electrical Impedance Method

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Liquid Sequence —— Key points
 Drive power
 (e.g.: syringe, liquid pump etc.)
 Start point
 (e.g.: lyse bottle, counting bath etc.)
 End point
 (e.g.: counting bath, waste container etc.)
 Path
 (e.g.: start point → SV1→ LP→ end point）
 Volume
 (e.g.: 100uL)
SV1
SV3
SV2
LP
schematic
Syringe

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Liquid Sequence —— Sample aspiration（CBC+DIFF）
9uL: DIFF bath
6uL: WBC/HGB bath
5ul drain out
Air
Diluent
40uL: DIFF bath
40uL: WBC/HGB bath
Air
Diluent
180ul diluent
20ul blood
Whole blood mode Pre-diluted mode

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Liquid Sequence —— Sample aspiration（CBC）
6uL: WBC/HGB bath
9ul drain out
Air
Diluent
40uL: WBC/HGB bath
Air
Diluent
180ul diluent
20ul blood
Whole blood mode Pre-diluted mode

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Liquid Sequence —— Sample distribution
DIFF WBC RBC

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Liquid Sequence —— Reagent Volume Required
Whole blood mode
0.135ml
LEO(II)
LEO(I)
Diluent
LH
Diluent
9ul
6ul
BC-5300(20ul)
BC-5380(20ul)
2.5ml
1.2ml
DIFF(1:138)
WBC(1:417.7)
0.5ml
2.5ml
WBC(1:500)
RBC(1:20000)
WBC/HGB Detection
RBC/PLT Detection
52.08ul
Flow Cell
DIFF Detection

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Liquid Sequence —— Reagent Volume Required
Pre-diluted mode
0.135ml
LEO(II)
LEO(I)
Diluent
LH
Diluent
40ul
Pre-Diluted Sample
80ul(1:10)
2.5ml
1.2ml
DIFF(1:138)
WBC(1:625)
0.5ml
2.5ml
WBC(1:750)
RBC(1:26040)
WBC/HGB Detection
RBC/PLT Detection
60ul
Flow Cell
DIFF Detection
40ul

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J27-T89-J28
J29-T101-J30
T99
T90
T91
T92
T93
T62
WBC
T102
T103
T104
T105
T56
T55
T127
T126
T54
T10
T43
J23-T42-J24
T41
T32
T37
J9-T13-J10
T25
T59
T57
T58
T66
J21-T40-J22
T85
T87
T86
T97 T109
T116
T117
T115
SV17
T122
T123
T128
SV18
SV15
SV16
SV14
SV02
SV03
SV01
SV23
SV12
SV24
SV11
RBC SV20
SV26
SV27
SV21
PV28
J17-T22-J18
RV
SV04 SV05 SV06
T69
T81
Transducer
J1-T11-J2
J3-T12-J4
T16
J39-T14-J11
J40-T15-J12
J16-T17-J41
DILUENT
LH LYSE
LEO（II） LYSE
LEO（I） LYSE
T5
T6
T7
T140
T33
T9
T8
J5-T18-J6
T68
T19
T79
SV10
T50
(T21)
T130
T145
T142
T141
试剂检测
DIFF
Chamber
Wipe Block
SPB
Waste
Container
LP3
LP2
C42
C10
C31
C11
C43
C9
C34
C26
C15
T144
C39
C40
T114
T111
J35-T139-J36
J31-T137-J32
T143
Isolation Chamber Isolation Chamber
Isolation Chamber
C20
C21
C22
C23
T121
T129
T120
J13-T20-J14
J19-T24-J20
J7-T39-J8
T84
T80
LEO(I)
LEO(II)
LH
J33-T138-J34
T98
T113
Shielding Cover
Shielding Cover
(T88)
SV25
LF1
(T100)
T28
LF2
T30
C45
C47
C48
C46
C49
C51
C52
C50
T53
T63
T94
SV22
T108
SV19
T65
T118
T107
T95
T110
T34
T45
T51
J25-T26-J26
T47
T96
T106
T36
T38
T64
C36
T46 T60
T77
T67
T82
T83
T61
T3
T2
T1
(T23)
SV07
T78
T112
T131
T35
PC
T71
Transducer
T70
T136
GP
T75-T76
T72
T74
T73 -T132
T134
T133
T135
T27
T49
T29
T31
T146
VC
Tied
J15
GF1
GF2
C1
C2
C3
C4
C5
C8
C12 C13
C14
C16
C17
C18
C19
C24
C25
C27
C28
C29
C30
C32 C33
C35
C37
C38
C41
C44
T119
C53 C54 C55
C56 C57 C58
C63
C59
C60
C61
C62
Flow Cell
LEO(I)
LEO(II)
LH
(T147)
(T148)
SV13
T149
T150
T48
C64
C65
DH
T152
T151
C67
C66
T153
T154
TS
SV09
T155
T156
C68
C69
C70
T157
T158
C71
T159
C72
T161
C74
T160
C73
J37-T52-J38
T163
T162
C75 C76
LYSE(2.5mLX3)
SP(250uL)
SH(10mL)
ASP(100uL)
DIL(10mL)
T164
J42-T165-J43
T166
T167
T168
C77
SV08
C78
C79
T146
tied
T4
T169
T170
T171
C80
C81
DIL
T172
C82
T173
C83
J44-T174-J45 T175
C84
Liquid Sequence —— Hydraulic Diagram
Sample
Aspiration
WBC
Channel
RBC
Channel
DIFF Channel
Lyse
Syringes
Pressure
&
Vacuum

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Liquid Sequence —— Sample Aspiration
T56
T55
T127
T126
T54
T57
SV02
SV03
SV01
T50
Wipe block
SPB
LP2
SV25
LF1
T53
T51
(T147)
SV13
T149
T48
T162
ASP(100uL)
DIL(10mL)
Waste
DIL

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Liquid Sequence —— Lyse Syringes
T10
SV04 SV05 SV06
T5
T6
T7
T9
T8
LYSE(2.5mLX3)
LEO(I)
LEO(II)
LH
DIFF
WBC

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Liquid Sequence —— Pressure & Vacuum
SV12
SV11
SV20 SV26
SV21
Transducer
SV10
PC
T71
Transducer
T70
T136
GP
T134
T133
T135
VC
GF1
GF2
C41
SV09
废液桶
SV8

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Liquid Sequence —— DIFF Channel
C5
T43
J23-T42-J24
T41
T32
T37
T25
J21-T40-J22
SV17
SV18
SV15
SV16
SV14
PV28
RV
J1-T11-J2
J3-T12-J4
T16
J39-T14-J11
J40-T15-J12
J16-T17-J41
T33
J5-T18-J6
T68
T19
SV10
(T21)
DIFF池
C26
C15
Isolation Chamber
J13-T20-J14
J19-T24-J20
J7-T39-J8
T28
LF2
T30
T34
T45
J25-T26-J26
T47
T36
T38
T46
T35
T29
T31
C4
C13
C25
C27
C28
C29
Flow Cell
SV13
T149
T150
C64
SP(250uL)
SH(10mL)
T164
J42-T165-J43
T166
C77
SV08
J44-T174-J45
VC
PC
DIL
LEO I
LEO II
Sample Reaction
Pre-Cleaning
Sample Preparing
Sheath Fluid Formation
Auxiliary Dispensing
Counting

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Liquid Sequence —— DIFF Channel
C5
T43
J23-T42-J24
T41
T32
T37
T25
J21-T40-J22
SV17
SV18
SV15
SV16
SV14
PV28
RV
J1-T11-J2
J3-T12-J4
T16
J39-T14-J11
J40-T15-J12
J16-T17-J41
T33
J5-T18-J6
T68
T19
SV10
(T21)
DIFF池
C26
C15
Isolation Chamber
J13-T20-J14
J19-T24-J20
J7-T39-J8
T28
LF2
T30
T34
T45
J25-T26-J26
T47
T36
T38
T46
T35
T29
T31
C4
C13
C25
C27
C28
C29
Flow Cell
SV13
T149
T150
C64
SP(250uL)
SH(10mL)
T164
J42-T165-J43
T166
C77
SV08
J44-T174-J45
VC
PC
DIL
LEO I
LEO II
Cleaning

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J27-T89-J28
T99
T90
T91
T92
T93
T62
WBC
T58
T97
T116
T117
T128
SV23
SV12
SV26
SV21
Transducer
T130
T145
Waste
Container LP3
LP2
C10
C11
C43
C39
C40
Isolation Chamber
C20
C21
T121
T129
T120
T84
T98
Shielding Cover
(T88)
T63
T94
SV22
T118
T95
T96
T82
T61
SV07
PC
T136
C8
C18
C32
T119
C63
C61
C62
DH
T152
T151
C67 T153
C70
T157
DIL
LH
DIL
T75-T76
T74
J15
C3
C16
T71
Transducer
T70
GP
T72
T73 -T132
T134
T133
T135
GF1
GF2
C37
C41
C30
T77
SV09
T155
T156
C68
VC
Liquid Sequence —— WBC/HGB Channel
Sample Preparing
Counting

17. © 2013 Mindray Confidential
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J27-T89-J28
T99
T90
T91
T92
T93
T62
WBC
T58
T97
T116
T117
T128
SV23
SV12
SV26
SV21
Transducer
T130
T145
Waste
Container LP3
LP2
C10
C11
C43
C39
C40
Isolation Chamber
C20
C21
T121
T129
T120
T84
T98
Shielding Cover
(T88)
T63
T94
SV22
T118
T95
T96
T82
T61
SV07
PC
T136
C8
C18
C32
T119
C63
C61
C62
DH
T152
T151
C67
T153
C70
T157
DIL
LH
DIL
T75-T76
T74
J15
C3
C16
T71
Transducer
T70
GP
T72
T73 -T132
T134
T133
T135
GF1
GF2
C37
C41
C30
T77
SV09
T155
T156
C68
VC
Liquid Sequence —— WBC/HGB Channel
Cleaning

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Liquid Sequence —— RBC/PLT Channel
J29-T101-J30
T102
T103
T104
T105
T59
T66
T109
T116
T115
T128
SV24
SV11
RBC SV20
SV26
Transducer
T130
T145
Waste
Container
LP3
LP2
C10
C11
C43
Isolation Chamber
C22
C23
T121
T129
T120
T84
T113
Shielding Cover
(T100)
T108
SV19
T65
T107
T110
T106
T67
T82
SV07
C30
C33
C63
C61
C62
DH
T151
T153
T154
TS
SV09
T155
T156
T158
C71
DIL
DIL
PC
T71
Transducer
T70
T136
GP
T72
T74
T73 -T132
T134
T133
T135
GF1
GF2
C37
C41
VC

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Liquid Sequence —— Start up
Initialize Liquid Parts
Test Vacuum
Test Pressure
Test WBC Volumetric Tube filer
Test RBC Volumetric Tube filer
1.Clean
2.Shut Down Abnormally:
Clean Twice
3.After Pack-UP:
First Prime, Then Clean
Background Test

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Liquid Sequence —— Shut down and Pack up
Clean
Use Cleanser
to Maintance
Shut Down Pack Up
Remove All Reagents
Empty the Tubing
Connect Diluent Tube to Distill Water
Clean the Tubing Via Distill Water
Empty the Tubing

21. © 2013 Mindray Confidential
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Version
Version Author Date Description
1.0 Tata Ren 13/AUG/2014
BC-5300(new)/BC-5380(new)
Liquid System First Release

22. © 2013 Mindray Confidential
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Verification
Version Verifier Date Comments
1.0 Mars Zhang 13/AUG/2014 Approved