This document summarizes a study assessing the effects of integrated vegetation management (IVM) practices on pollinator and botanical communities in rights-of-way (ROW) habitats. The study involved butterfly, bee, and vegetation surveys along transects in ROWs to track biodiversity changes over three seasons. Results showed various pollinator groups including butterflies, bees, flies and beetles present in the ROWs. Milkweeds and nectar resources varied by season. Habitat composition was biased towards pollinator habitats. Future directions include increasing data resolution, using baseline data to track long-term changes, and emphasizing training to improve survey consistency. The study demonstrates that ROWs can provide valuable wildlife habitat when
Breakout Session Slides
Climate Adapted Native Plant Materials Project: Practical Innovation for an Uncertain Future – Mike Conroy will discuss how the Tualatin SWCD is evaluating assisted migration to augment the genetic fitness of native plants used in restoration projects. The core of this project is a long-term common garden experiment.
Monday, February 12 3:05 - 3:30 p.m.
What flowers do our honeybees visit? Dr Anna Oliver of the Centre for Ecology & Hydrology explains how the National Honey Monitoring Scheme in the UK is using citizen science to help us understand honeybees and what honey reveals about the wider environment.
Find out more at https://honey-monitoring.ac.uk
Breakout Session Slides
Climate Adapted Native Plant Materials Project: Practical Innovation for an Uncertain Future – Mike Conroy will discuss how the Tualatin SWCD is evaluating assisted migration to augment the genetic fitness of native plants used in restoration projects. The core of this project is a long-term common garden experiment.
Monday, February 12 3:05 - 3:30 p.m.
What flowers do our honeybees visit? Dr Anna Oliver of the Centre for Ecology & Hydrology explains how the National Honey Monitoring Scheme in the UK is using citizen science to help us understand honeybees and what honey reveals about the wider environment.
Find out more at https://honey-monitoring.ac.uk
Crop wild relatives - looking at trends in genetic diversity to inform conser...Bioversity International
Presentation given by Elena Fiorino, Imke Thormann and Ehsan Dullo from Bioversity International on the closing day of the International Horticultural Congress 2014.
In their presentation they tackle questions such as 'Why is in situ conservation of crop wild relatives important?' and 'How can we develop in situ conservation strategies?'
Watch this video to learn more about crop wild relatives and why they are the cornerstone of agriculture: https://www.youtube.com/watch?v=Ah7RruMZ9CU
Prof Richard Pywell of the Centre for Ecology & Hydrology is a co-author (with Marek Nowakowski) of Habitat Creation and Management for Pollinators. This freely available guide outlines simple actions for land managers to take to improve the environment for pollinators. Richard's presentation at the launch event for the guide explains more about the years of applied pollinator research that has informed the publication.
See more: http://www.ceh.ac.uk/news-and-media/blogs/pollinators-research-and-practical-management-white-coat-and-welly-boot
Horizon scanning for emergence of new viruses in animal and public healthEFSA EU
Presentation of the EFSA's second scientific conference, held on 14-16 October 2015 in Milan, Italy.
DRIVERS FOR EMERGING ISSUES IN ANIMAL AND PLANT HEALTH
NATIONAL GREEN CORPS AND NATIONAL SERVICE SCHEME CLIMATE EDUCATION AND BIODIV...W G Kumar
A training module to introduce College Lecturers and School Teachers to the subject of Climate Education and Live Projects that they can do in their institution and elsewhere
Crop wild relatives - looking at trends in genetic diversity to inform conser...Bioversity International
Presentation given by Elena Fiorino, Imke Thormann and Ehsan Dullo from Bioversity International on the closing day of the International Horticultural Congress 2014.
In their presentation they tackle questions such as 'Why is in situ conservation of crop wild relatives important?' and 'How can we develop in situ conservation strategies?'
Watch this video to learn more about crop wild relatives and why they are the cornerstone of agriculture: https://www.youtube.com/watch?v=Ah7RruMZ9CU
Prof Richard Pywell of the Centre for Ecology & Hydrology is a co-author (with Marek Nowakowski) of Habitat Creation and Management for Pollinators. This freely available guide outlines simple actions for land managers to take to improve the environment for pollinators. Richard's presentation at the launch event for the guide explains more about the years of applied pollinator research that has informed the publication.
See more: http://www.ceh.ac.uk/news-and-media/blogs/pollinators-research-and-practical-management-white-coat-and-welly-boot
Horizon scanning for emergence of new viruses in animal and public healthEFSA EU
Presentation of the EFSA's second scientific conference, held on 14-16 October 2015 in Milan, Italy.
DRIVERS FOR EMERGING ISSUES IN ANIMAL AND PLANT HEALTH
NATIONAL GREEN CORPS AND NATIONAL SERVICE SCHEME CLIMATE EDUCATION AND BIODIV...W G Kumar
A training module to introduce College Lecturers and School Teachers to the subject of Climate Education and Live Projects that they can do in their institution and elsewhere
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
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Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Cancer cell metabolism: special Reference to Lactate Pathway
Assessing Botanical and Pollinator Communities in ROW Habitats
1. Assessing Botanical and Pollinator
Communities in ROW Habitats
Adam Baker, Davey Institute
Scott Eikenbary, Davey Resource Group
2. Assessing the effects of IVM on pollinator and botanical communities
in ROW habitats
Adam M. Baker, PhD
Davey Institute
Technical Advisor, Pollinator Ecologist
Adam.Baker@davey.com
Scott Eikenbary
Davey Resource Group
Project Manager
Scott.Eikenbary@davey.com
10. Monarchs use special overwintering grounds
Revealed to the world in August 1976
issue of National Geographic
11. All Hands on Deck!
Contributions from all land use sectors
are needed to restore enough milkweed
12. All hands on deck to plant milkweeds!
Farm reserve lands Roadsides
Rights-of-way Urban/suburban gardens
13. Row as habitat
• Creates connectivity
• Natural flyways
• Migration/Highways
• Invasive species
• Pesticide exposure
• Disease transmission
ROWs have great potential for habitat
14. Benefits of IVM
● Ensure safety, compliance, and improved
reliability
● Compatible plant communities managing
vegetation, promoting biodiversity
● Environmental & ecological sustainability
and improved habitat quality
● Reduced maintenance costs overtime
● Creating partnerships and positively
impacting local communities
15. Long lasting management
strategies
● Rights-of-ways become assets, not
liabilities
● Long term sustainability
● Meet and exceed program objectives
● Collaborate, innovate, with boots on the
ground expertise using scientifically
accepted techniques
● Results = safety, compliance, desirable
vegetation and wildlife
16. Adaptive Management
● Not all sites are the same, changes
occur over time
● Observe, document, and adjust
● Monitor annually and seasonally
● Design methods to reflect objectives
and inform decision making
17. ● Voluntary agreement
● Provides assurances that no further conservation measures or
restrictions will apply if a species is listed
● Contribute net conservation benefits and address key threats under
property owners’ and land managers’ control
19. Pollinators as an umbrella
species
● Indirectly protect other species of ecological communities
○ Mammals
○ Birds
○ Reptiles
○ Amphibians
○ Arthropods
20. Methods: Surveys
Pollinator and botanical surveys
• Transects 10 x 150’
• Surveyed 3 times per year
- spring (24 – June 14)
- summer (July 11 – July 31)
- late summer (August 24 – September 14)
• Track changes in biodiversity
over time in relation to IVM
practices
21. Methods: Butterfly counts
*Pollard 1977
Pollard Cube method
• Envision cube dimensions
• Slowly walk just outside
transect (3 min approx.)
• Record butterflies as
they pass through
the cube
22. Methods: Bees and other foraging pollinator counts
At each of the 3 sampling dates
• 7-15 min
• Count all bees
- Honey bees
- Bumble bees
- Carpenter bees
- other bees
• Count all other pollinators interacting
with flowers within the transect
- beetles
- flies
- moths
- wasps
23. Surveys in spring, summer, and late summer
● Number of plants that are currently blooming: herbaceous plants
and shrubs, not vines
● Number of species of plants currently blooming
● Total number of plants of all species that are currently blooming
Methods: Vegetation survey
27. Results: Pollen and nectar resources throughout the season
Fig 3: Average flowering plants in bloom for each site by
sampling date. Data is represented as mean ± SE.
31. Discussion: Habitat composition/Pollinator Scorecard Tier 2
• Bias towards pollinator habitat
• Weighted heavily on milkweed presence
• Performing once per year can be misleading
• 3 times per year
• Revision for 2023!
32. Discussion: Future directions
• Meeting compliance standards
• Increase resolution of data collection
- Andrenidae
- Colletidae
- Halictidae
- Megachilidae
• Baseline data used to track changes over time
• Introductory and advanced trainings for observers
33. Emphasis on Training
● On paper, protocols seem easy
● Insect ID
● Botanical ID
● Invasive species
● Develops consistency
● 2 Trainings per year
● Field and lab experience
34. Final thoughts
● Unique and valuable opportunities for
pollinators and wildlife habitat
● ROW’s are now assets!!
● Safety, compliance, reliability, and
environmental stewardship
● Advance business practices, reduced
costs
● Sustainable habitat that can act as
conservation reservoirs
35. Thank you!
“When we see land as a community to which we belong, we may begin
to use it with love and respect.” - Aldo Leopold
Questions?
Editor's Notes
UVM, incompatible plant species are the pests we’re trying to manage
Compatible/desirable vegetation that leads to early successional pollinator habitat that isn’t a threat to interfere with conductors, grass, forbs, shrubs, bio control
Flexible and adaptive, not tied to one or two methods
Native spp keeping invasives and incompatibles away = healthy ecosystems
ROW’s as habitat
Low risk, high reward - conservation, regulatory requirements, reduced costs
make adjustments needed
Engage stakeholders
Low risk, high reward - conservation, regulatory requirements
Habitat conservation plans costly, disrupt projects
Required field monitoring to aid in ivm program improvements
3rd party recognition for meaningful wildlife habitat management efforts, continuous improvements
Impactful long term commitments - conservation, education
Increasing habitat/protecting pollinators indirectly protects other species in ecological communities
Not just butterflies and bees (beetles, moths, mosquitoes) - some species are not able to rep w/out certain pollinators
Pollinators are ultimately responsible for the seeds and fruits of many different types of plants that feed mice, birds, deer, bears
Maybe even the bucks
Rows offer
c
Low risk, high reward - conservation, regulatory requirements, reduced costs