This study aims to generate fusion-defective mutants of Chlamydomonas reinhardtii through random insertional mutagenesis using glass bead transformation. Transformed cells expressing paromomycin resistance are selected and screened for inability to mate. Several mating-deficient clones have been identified that are defective in swimming, agglutination or both. Further analysis will determine if any clones possess a fusion defect and identify the insertion location to study genes involved in fusion.