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3. Account for the inability of aerobes to grow in the absence of O 2 . 4. Account for the
subsurface growth of microaerophiles in a shake-tube culture. 5 . Consider the culture type in
which growth was distributed throughout the entire medium and explain why the growth was
more abundant toward the surface of the medium in some cultures, whereas other cultures
showed an equal distribution of growth throughout the tubes. 6 . Account for the fact that the C .
sporogenes culture showed a separation within the medium or an elevation of the medium from
the bottom of the test tube. 7. Your instructor asks you to explain why the Streptococcus species
that are catalase-negative are capable of growth in the presence of oxygen. How would you
respond?

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