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2. A broth culture of his Salmon typhimurium is inoculated onto an agar plate that does not contain histidine. A strongly mutagenic chemical is added, but no evidence of mutations shows up after incubation. Why? (Hint: Review Step 1 of the Ames test procedure and consider at which stage during the life cycle of a microbe a mutation would take place..

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2. A broth culture of his Salmon typhimurium is inoculated onto an agar plate that does not contain histidine. A strongly mutagenic chemical is added, but no evidence of mutations shows up after incubation. Why? (Hint: Review Step 1 of the Ames test procedure and consider at which stage during the life cycle of a microbe a mutation would take place.

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