2.  Diesel engine oil is the major product of crude
oil.
 It is a major source of pollution in our
environment.
 Petroleum is a aromatic compound.
 Oil is the Xenobiotic and recalcitrant.
 Diesel oil spills on agricultural land generally
reduced plant growth.
 Petroleum hydrocarbon can be degraded by
microorganism .

3.  A large number of organism capable of
degrading polyaromatic hydrocarbons
compound.
 Many different species , can degrade
petroleum compound such as pseudomonas,
micrococcus, bacillus, corneybactetium.
 Hydrocarbon degrading bacteria are widely
distributed in marine fresh water and soil
habitats.
 Oil degrading organism basically used alkane
as a energy source and help in bioremediation.

4. Aims and objective
The Aim of our present
study to isolate the
petroleum engine oil
degrading bacteria
from garage soil.
The Aim present study
is to identify and
evaluate their
degradation capability.

5. SAMPLE COLLECTION
 Sample collection from garage.
ISOLATION METHOD
 Spread plate method
 Streak plate method

6.  IDENTIFICATION
Biochemical testing
IMVIC test
Starch test
Urease test

7.  Gram staining negative
 Shape rod shaped
 Colony orange
 Urease negative
 Gelatinase positive
 H2s production negative
 Indole production negative
 Methyl red test negative
 Voges proskauer test positive
 Citrate test positive
 Lactose negative
 Sucrose positive
 Glucose negative
 Starch negative
 Casien hydrolysis positive
 Manni tol broth negative
 Identified Acinetobacter may be

8. Colony of organism is found on
the Enrichment media
Morphology of isolated strain after
gram’s staining

9. Urease agar test

10. Casein hydrolysis
test
Gelatin agar
test

11. conclusion
 Bacteria are able to grow on to the enriched
media and used diesel engine oil as a energy
source.
 The bacteria have high potential to degrading oil
and producing biosurfactant layer due to which
reduce a surface tension of soil and water.
 Isolated bacteria is Acinetobacter sp. may be.
 Biochemical test were performed with
susceptibility was done with bacterial isolates.
 It can be used to in big level of future purpose
using a gene &produce level for future.

12. FURTHER ASPECTS
 Soil sample can stored in -80c for further
analysis.
 carry out PCR and sequencing.
 identify 16s rna from potential bacteria.

13. THANK YOU