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1. You are studying Protein X in E . coli. You perform spheroplasting and centrifugation and
obtain immunoblot A on the LEFT, above. If you fused PhOA to the C-terminus of Protein X ,
can you predict the color of E . coli expressing this fusion when grown on medium with BCIP?
Explain why or why not. (1-2 sentences) 2. You are studying Protein Y in E. coll. You perform
spheroplasting and centrifugation and obtain immunoblot B on the RIGHT, above. If you fused
PhOA to the C-terminus of Protein Y , can you predict the color of E . coli expressing this fusion
when grown on medium with BCIP? Explain why or why not. (1-2 sentences)

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