Beginners Guide to TikTok for Search - Rachel Pearson - We are Tilt __ Bright...
Method of preparation of niosomal in situ gel
1. Method of preparation of Niosomal in situ gel
preparation of niosome:- Niosomes will be prepare by the thin film hydration method with
slight modification. We will be use span 60, Tween 60 as surfactant and Cholesterol with
solvent (chloroform and methanol). Dissolve the surfactant and cholesterol in the solvent
system and evaporate the solvent by using rotary evaporator at 120 rpm, 60 C° for 1-2 hrs.
After that the thin film will be form than keep it for overnight to remove the remaining solvent.
After that The N-acetyl cysteine (NAC) will be dissolve into the PBS than use this drug loaded
PBS to rehydrate the film and sonicate the dissolve film with 4 cycle of 180sec with 60 off.
Preparation of niosomal in situ gel:- We will use 1% w/w of PF 127 or Carbopol 934, 940.
Transfer the niosome into the gel.
Characterization of niosome
% Entrapment efficiency:- It is calculate to know how much drug get entrap into the niosome.
It is calculated by the formula.
Determination of particle size:- The determination of particle size of niosome will be done by
the zetasizer.
Polydispersity index (PDI):- The polydispersity index shows the how much your sample is
homogeneous. The value of PDI should be in between 0.1-0.4.
Determination of zeta potential:- Zeta potential is potential difference between colloid and
dispersing liquid. It is calculated by the zetasizer. It make sure that how stable your sample is.