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058 CULTURE OF FRUIT FLY (Drosophila melanogaster) 
FOR GENETIC STUDIES 
Fruit flies swarm in every orchard during the hot months. They thrive on the 
fruits that fall premature from the tree. August through October, you can 
also find them buzzing around any bowl of fruit on your kitchen table. They 
especially love bananas. 
MATERIALS REQUIRED 
glass jar 
Glass jar 
Cotton wool 
Ripe bananas 
Ripe bananas Cotton wool
The males are smaller than the females and shapes of the abdomens differ. 
The abdomen of the male is smaller, more rounded with a black tip. 
The abdomen of the female is larger, more pointed and has 4 or 5 black 
transverse rings. 
Male Female
Attract fruit flies by putting over-ripe 
fruit in an open container, e.g. a glass 
jar. After trapping the fruit flies, 
transfer them to small containers 
containing fruit chunks, preferably 
over-ripe banana. Put a slice of ripe 
fruit in the bottom of the container and 
make a paper funnel with a hole in the 
end to fit the mouth of the container. 
Place the container in the open.
When six or eight fruit flies have entered (including both males 
and females) remove the funnel and plug it loosely with cotton 
wool. 
Cotton wool
Soon eggs will be deposited, and in 2 or 
3 days the larvae will hatch. Put a piece 
of paper in the container for the larvae 
to crawl on when they are ready to 
pupate. 
Insects emerge from the pupae. 
Put newly-hatched fruit flies in another 
container to start a new generation. 
Young 
adults 
Larvae 
and 
pupae
Soon eggs will be deposited, and in 2 or 
3 days the larvae will hatch. Put a piece 
of paper in the container for the larvae 
to crawl on when they are ready to 
pupate. 
Insects emerge from the pupae. 
Put newly-hatched fruit flies in another 
container to start a new generation. 
Young 
adults 
Larvae 
and 
pupae

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058 culture of fruit fly for genetic studies

  • 1. 058 CULTURE OF FRUIT FLY (Drosophila melanogaster) FOR GENETIC STUDIES Fruit flies swarm in every orchard during the hot months. They thrive on the fruits that fall premature from the tree. August through October, you can also find them buzzing around any bowl of fruit on your kitchen table. They especially love bananas. MATERIALS REQUIRED glass jar Glass jar Cotton wool Ripe bananas Ripe bananas Cotton wool
  • 2. The males are smaller than the females and shapes of the abdomens differ. The abdomen of the male is smaller, more rounded with a black tip. The abdomen of the female is larger, more pointed and has 4 or 5 black transverse rings. Male Female
  • 3. Attract fruit flies by putting over-ripe fruit in an open container, e.g. a glass jar. After trapping the fruit flies, transfer them to small containers containing fruit chunks, preferably over-ripe banana. Put a slice of ripe fruit in the bottom of the container and make a paper funnel with a hole in the end to fit the mouth of the container. Place the container in the open.
  • 4. When six or eight fruit flies have entered (including both males and females) remove the funnel and plug it loosely with cotton wool. Cotton wool
  • 5. Soon eggs will be deposited, and in 2 or 3 days the larvae will hatch. Put a piece of paper in the container for the larvae to crawl on when they are ready to pupate. Insects emerge from the pupae. Put newly-hatched fruit flies in another container to start a new generation. Young adults Larvae and pupae
  • 6. Soon eggs will be deposited, and in 2 or 3 days the larvae will hatch. Put a piece of paper in the container for the larvae to crawl on when they are ready to pupate. Insects emerge from the pupae. Put newly-hatched fruit flies in another container to start a new generation. Young adults Larvae and pupae