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Incubators
Autoclave
Refrigerator
pH meter
Balances
Water baths
Water distillation
Thermometers
Shaker
Hot air oven
Desiccator
Centrifuge
Compound
microscopes
Hot plate
Conical flasks
Beakers
Test tubes
Pipetts
Burettes
Petri dishes
Slides
Cover
Cork borers
Inoculating loop needle
Scissors
Forceps
Benzen flame
Label papers
Sterility
Basket
Test tube
Stainless steal
cylinder
Filter paper
Cotton
Swabs
Cloth
Media
-Colony - Colonies
Culture
Subculture
Sample
Incubation
temperature
Infection
Disinfectant
Air pollution
Water Pollution
Inoculum
growth media
medium
––––
–––-
chemically defined media(
chemicallymeadianonedefinde
solid media
semisolid media
liquid media
enriched media
selective media
-differential media
-:biological assay media
-media for enumeration of bacteria
media for characterization of bacteria
water
peptone
extractPeef
solidifying agents
gelatin
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agaragar
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
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-
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Nutrient agar-
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–
Nutrient agar
–-
Pure culture
Mixed culture
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--
-form
-Elevation
-Surface
-Smooth
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-concentrically ringed
-Radiantly ridged
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-Optical
features
-Opaque
-Translucent
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-Opalescent
-Consistency
-Butyrous
-Viscid
-Membranous
-Brittle
-:Pigmentation
-
-
-
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-
-
-coccus
-coccus
-Diplococci
-Tetracocci
-Sarcina
-Streptococci
-Staphlococci
-Bacillus
-E.coli
-Diplibacillus
-Streptobacillus
-
-Spirillum
-Vibrio


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–
Negative Stain
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India ink)

Nigrossin).

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cholera
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plague
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Endospore
stining
BacillusClostridium
Endospore
Schaeffer and Fulton
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--
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Fulton
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Malachite-green
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-
-
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
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Environmental influences .
-
Effect of temperature on groth :
Optimum
Minimum
-
-
E.coli
-Bacillus
subtilis
-
-
-
-
-
Bacillus
mesophiles
-
Bacillus
subtilis
-
Effect of H-ion concentration
optimum
maximum
minimum
-Bacillus
-
pH
-
-
-
Bacillus
subtilis
Effect of osmotic pressure
Plasmoptysis ,
Plasmolysis .
salt
toleranthalophilic
-Bacillus subtilis-
-
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-
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B.subtilis
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Bacillu
s
Effect of aeration
strict aerobes
faculative anaerobes
strict
anaerobes
microaerophilic
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soil suspension
-
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O O
Oxygen molecule

hydrolases
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
amyloseAmylopectin


Bacillus subtilis

Collagen

Bacillus subtilis

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