MVPK cell cultures showed cytopathic effect when inoculated with the virus. Animals inoculated with the virus showed increased rectal temperatures within 24 hours and vesicular lesions at inoculation sites by 48 hours, preceded by fever. Complement fixation and serum neutralization tests were performed on convalescent serum samples to detect antibodies against the virus. RT-PCR and agar gel precipitation tests were also conducted but not described in detail. References included several textbooks on veterinary virology, bacteriology, and the diseases of swine.