This document summarizes research on uniaxial tensile testing of concrete cores from existing structures. Tests were conducted on cores from the Kiruna Bridge in Sweden and other structures to determine fracture properties. Results show that for older concretes, the fracture energy GF correlates well with the formula GF = 73·fc0.18 from codes, with covariances of 19-53%. For newer high-strength concretes, there is more variation in GF. Stresses parallel to cracks were also found to influence fracture and need further study. The research aims to better understand concrete fracture properties in existing structures.
EXPERIMENTAL STUDY ON FLEXURAL BEHAVIOR OF THE SELF-COMPACTING CONCRETE WITH ...IAEME Publication
This Research studies, In recent years, Self-compacting concrete (SCC) can be considered as a concrete which has little resistance to flow so that it can be placed and compacted under its own weight with little or no vibration effort, yet possesses enough viscosity to be handled without segregation or bleeding. Several tests such as slump flow, V-funnel, L-box has carried out to determine optimum parameters for the self-compatibility of mixtures. In this article SCC plain and
SCC hybrid fibres has compared. The current study includes a practical program considers the effect of adding Nylon e-300 fibre and Nylon tuff fibre to structural behavior of self-compacting concrete such as compressive strength and flexural strength behavior represent by mix proportion-strength
curves.
EXPERIMENTAL STUDY ON FLEXURAL BEHAVIOR OF THE SELF-COMPACTING CONCRETE WITH ...IAEME Publication
This Research studies, In recent years, Self-compacting concrete (SCC) can be considered as a concrete which has little resistance to flow so that it can be placed and compacted under its own weight with little or no vibration effort, yet possesses enough viscosity to be handled without segregation or bleeding. Several tests such as slump flow, V-funnel, L-box has carried out to determine optimum parameters for the self-compatibility of mixtures. In this article SCC plain and
SCC hybrid fibres has compared. The current study includes a practical program considers the effect of adding Nylon e-300 fibre and Nylon tuff fibre to structural behavior of self-compacting concrete such as compressive strength and flexural strength behavior represent by mix proportion-strength
curves.
Design of Wind Generated Structural Response of a Tall Building Situated in T...Rakibul Hasan,MEng,EIT
•Designed a 90 m tall building by calculating design Wind Speed with Gumbel Distribution.
•The structural response was measured by the bending moment, the peak top deflection, and the maximum amplitude of top acceleration, which were calculated following the dynamic analysis method as outlined in the NBCC (National Building Code of Canada). In order to mitigate the excessive structural response, the building density was recommended to be increased by thickening the slab and shear walls.
Aninvestigation into the effects of water containated with chloride salts on ...Samson Olakunle OJOAWO
Paper presented at the 2nd International Conference on Engineering and Technology Research (FET
Conference 2013), LAUTECH, Ogbomoso, Nigeria, March 26-28, 2013.
An Experimental Study on Fresh and Hardened Properties of Self Compacting Con...SJ BASHA
M.tech project
Tittle: An Experimental Study on Fresh and Hardened Properties of Self Compacting Concrete with Marble Powder and Cement Kiln Dust As Mineral Admixture
Authors: Syed Jeelani Basha, K Sailaja
Subject: Advance Concrete Technology, Self compacting concrete , mineral and chemical admixtures.
Scope: Further studies on recycle of marble powder and cement kiln dust
College: Gudlavalleru Engineering College, Gudlavalleru, Krishna dist., Andhra Prades, India.
Design of Wind Generated Structural Response of a Tall Building Situated in T...Rakibul Hasan,MEng,EIT
•Designed a 90 m tall building by calculating design Wind Speed with Gumbel Distribution.
•The structural response was measured by the bending moment, the peak top deflection, and the maximum amplitude of top acceleration, which were calculated following the dynamic analysis method as outlined in the NBCC (National Building Code of Canada). In order to mitigate the excessive structural response, the building density was recommended to be increased by thickening the slab and shear walls.
Aninvestigation into the effects of water containated with chloride salts on ...Samson Olakunle OJOAWO
Paper presented at the 2nd International Conference on Engineering and Technology Research (FET
Conference 2013), LAUTECH, Ogbomoso, Nigeria, March 26-28, 2013.
An Experimental Study on Fresh and Hardened Properties of Self Compacting Con...SJ BASHA
M.tech project
Tittle: An Experimental Study on Fresh and Hardened Properties of Self Compacting Concrete with Marble Powder and Cement Kiln Dust As Mineral Admixture
Authors: Syed Jeelani Basha, K Sailaja
Subject: Advance Concrete Technology, Self compacting concrete , mineral and chemical admixtures.
Scope: Further studies on recycle of marble powder and cement kiln dust
College: Gudlavalleru Engineering College, Gudlavalleru, Krishna dist., Andhra Prades, India.
Similar to D2 (A6) Lennart Elfgren - Uniaxial tensile test method for cylindrical concrete cores from existing structures.pptx (20)
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
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from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
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In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
D2 (A6) Lennart Elfgren - Uniaxial tensile test method for cylindrical concrete cores from existing structures.pptx
1. XXIV NCR Symposium 2022-08-16—19, Stockholm
Uniaxial tensile test method for cylindrical concrete cores
from existing structures
1 (20)
Jonny Rasoul Lennart
Nilimaa Nilforoush Elfgren
1 (20)
2. Contents
• Background
• Tests on Kiruna Bridge
• Test results. Evaluation of fib recommendation
• Stresses parallel to crack
• Conclusions
2 (20)
3. Background 3 (20)
Load controll – Brittle Deformation controll - Softening 3-point bend test
GF A = W0 = Energy [NM] → GF = Fracture Energy [Nm/m2]
4. Brittleness and size effect
lch = fct
2/EGF = characteristic length
The brittleness decreases when the fracture energy increases
Size effect: Large structures are more brittle than small ones
4 (20)
5. Built 1959, Tested 2014, Length 121,5 m
Concrete 40 MPa, Tests 62,3 MPa, Reinforcement 400, 600 MPa
Prestress BBRV 1450/1700 MPa
Example -Kiruna Bridge – Test to failure
5 (20)
7. Sensitivity study of FEM Model
The value of GF
chosen in FEM
models has a big
impact on results
Bagge, Niklas (2017) PhD Thesis
Prestress
Strengthening
Boundary Conditions
Elaticity
Poisson’s ratio
Tensile strength
Compressive strength
Fracture Energy
Fixed to rotation crack
Crack band width
Tension stiffening
Aggregate size
Shear stiffness reduction
Compression strength red.
7
14. Code proposals
𝐺𝐹 = ∝𝐹
𝑓𝑐
𝑓𝑐0
0.7
aF = 20 for dmax = 8 mm
30 for dmax = 16 mm
50 for dmax = 32 mm
GF = 73·fc
0.18
CEB-FIB MC 1990
fib MC 2010, 2020
14 (20)
19. Conclusions
• For old Swedish concretes (30 – 50 years) GF ≈ 73·fc
0.18
fib Model Code 2010/2020. Covariances from 19 to 53%.
• For new concretes (ages up to a few months) variations are larger,
especially for high strength concretes with compression strengths fc > 80
MPa.
• Results are from uniaxial tests on drilled out cores. Beams in three-point
bending are simpler to test. However, beams are complicated to saw out
from existing structures, so here drilled out cores are preferable.
• The influence of stresses parallel to crack ought to be studied
19 (20)
20. Acknowledgements
Many thanks for your kind attention
Jonny.Nilimaa@ltu.se
Rasoul.Nilforoush@ltu.se
Lennart.Elfgren@ltu.se
20 (20)
Swedish Universities of the Built
Environment
(LTH, KTH, Chalmers, LTU, RISE)