The document summarizes experiments analyzing the molecular interactions between Factor VII and Tissue Factor that are important for their binding. It finds that the extracellular domain of Tissue Factor and calcium ions play key roles in the binding affinity between Factor VII and Tissue Factor in solution. Removing part of Factor VII or the extracellular domain of Tissue Factor reduces their binding affinity.

1. Kori Jackson Paper 4 Flow Chart 4/7/2014
What Asked: What molecular interactions are integral for the binding of Factor VII to Tissue Factor?
How Asked: What molecular (protein-protein) interactions take place in solution and are integral to in
solution behavior of Factor VII and it’s binding to VIIa and TF. Which domains are important, and how
does Ca2+
affect binding affinity of this system.
Figure 1: Analysis of binding tissue factor complesxes with non-inhibitory anti-tissue factor This was to
see the TF-VII interaction in solution. Binding of TF complexes to anti-tissue factor not affected by
presence of VII. Authors claim that binding of TF complexes to anti-tissue factor reflects concentration of
complexes formed in solution.
Figure 2: Analysis of factor VII binding to Tissue Factor and extracelluar Tissue Factor domain (TF:1-219)
 binding of VII to TF:1-219 had a 10-fold higher Kd than regular TF.
Figure 3: Analysis of des-(1-38)-VII binding to TF and TF:1-219. binding was tighter for TF:1-219 than
regular TF.
Figure 4: Analysis of tissue factor extracellular domain with and without calcium cation.  Low affinity
interaction of VII and TF is dependent on calcium presence, this affect was reversed by presence of EDTA
in lieu of calcium.
Figure 5: Comparing hydrolysis of peptide in VIIa versus des-(1-38)-VIIa cannot interpret figure, legend
to graph representation not clear.
Figure 6: Analyzing proteolytic activation of fX by VIIa versus des-(1-38)-VIIa Activation of fX appears
higher with TF than truncated TF, though figure legend to graph representation not clear.
Conclusion: Author believes that hypotheses stating multiple interactions systems for the binding of VII
and TF are supported. Myself, not so sure.