ViaGlue Example Applications

1. 25µm 25µm
D
A B
25 µm
C
100µm
F G
100µm
H
50µm
100µm
E
Rapid and Stable 3D Liver Tissue
AlbuminSecreon(µg/day/millionhepatocytes)
B C
Cell Culture
A
Liver func on assay
Drug
0
10
20
30
40
50
60
0 2 4 6 8 10 12 14
Mix 3D
Hep 3D
Mix 2D
Hep 2D
Cell Culture Time (Day)
Mix 3D Hep 3D Mix 2D Hep 2D Mix 3D Hep 3D Mix 2D Hep 2D
Mix 3D Hep 3D Mix 2D Hep 2D
0"
2"
4"
6"
8"
10"
12"
14"
16"
CytochromeP4503A4foldacvaon
Functional 3D Liver TissueComplex Spheroid Formation
Examples of Cell Assembly

2. Cardiomyocytes
Fibroblasts HUVECs
Functional 3D Cardiac Tissue with ViaGlue
2D Co-Culture
3D Co-Culture Cardiac Tissue
ViaGlue BViaGlue A

3. 2D Cardiomyocytes
2D Mixed
3D Cardiomyocytes
3D Mixed
Fluorescent antibody staining of tissues for expression of
cardiac markers (cardiac troponin T and connexin 43). Scale
bar: 80µM
DAPI
cTnT
Cxn 43
3D Cardiac Tissue Assembled with ViaGlue

4. 2D
Cardiomyocytes
0.92s
3D
Cardiomyocytes
0.64s
2D Mix
0.78s
3D Mix
0.83s
Time interval for tissue beats
Various Cardiac Tissue Assemblies Spontaneously Beat
(no external electrical stimulation)
Scaffold free ViaGlue allows high cell contact density
for large tissue synchronous beating

5. A
C
B
D
I
K L
J
O P
E F M N
G H
Examples of Various Cell Assemblies Generated by ViaGlue

6. Generation and analysis of 3-Dimensional Liver Tissue formed via an Inter-cell Bio-orthogonal Mediated Assembly
Process. Submitted.
A Dual Receptor and Reporter for Multi-Modal Cell Surface Engineering. W.Luo, N. P. Westcott, D. Dutta, A. Pulsipher,
D. Rogozhnikov, J. Chen, M.N. Yousaf*. ACS Chemical Biology, 2015, 10, 2219–2226.
Spheroid and Tissue Assembly in Microfluidic Flow. P.J. O’Brien, W. Luo, D. Rogoznikov, J. Chen, M.N. Yousaf*.
Bioconjugate Chem. 2015, 26, 1939-1949
Remote Control of Tissue Interactions via Engineered Photo-switchable Cell Surfaces. W. Luo, A. Pulsipher, D. Dutta, B.
M. Lamb, M. N. Yousaf*. Nature Scientific Reports 2014, 4, 6313.
Cell surface engineering by a conjugation and release approach based on the formation and cleavage of oxime
linkages upon mild electrochemical oxidation and reduction. A. Pulsipher, D. Dutta, W. Luo, M.N. Yousaf*. Angew.
Chem. Int. Ed. 2014, 53, 9487-9492.
Engineering Cell Surfaces via Liposome Fusion. D. Dutta, A. Pulsipher, W. Luo, M.N. Yousaf*. Bioconjugate Chemistry.
2011, 22, 2423-2433. Chemical & Engineering News 2011, 49, 36.
Synthetic Chemoselective Rewiring of Cell Surfaces: Generation of Three-Dimensional Tissue Structures. D. Dutta, A.
Pulsipher, W. Luo, M. N. Yousaf*. J. Am. Chem. Soc. 2011, 133, 8704-8713. Science 2011, 332, 1011-1012.