Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.
25µm 25µm
D
A B
25 µm
C
100µm
F G
100µm
H
50µm
100µm
E
Rapid and Stable 3D Liver Tissue
AlbuminSecreon(µg/day/millionhepat...
Cardiomyocytes
Fibroblasts HUVECs
Functional 3D Cardiac Tissue with ViaGlue
2D Co-Culture
3D Co-Culture Cardiac Tissue
Via...
2D Cardiomyocytes
2D Mixed
3D Cardiomyocytes
3D Mixed
Fluorescent antibody staining of tissues for expression of
cardiac m...
2D
Cardiomyocytes
0.92s
3D
Cardiomyocytes
0.64s
2D Mix
0.78s
3D Mix
0.83s
Time interval for tissue beats
Various Cardiac T...
A
C
B
D
I
K L
J
O P
E F M N
G H
Examples of Various Cell Assemblies Generated by ViaGlue
Generation and analysis of 3-Dimensional Liver Tissue formed via an Inter-cell Bio-orthogonal Mediated Assembly
Process. S...
Upcoming SlideShare
Loading in …5
×

ViaGlue Example Applications

ViaGlue Example Applications

Related Books

Free with a 30 day trial from Scribd

See all
  • Be the first to comment

  • Be the first to like this

ViaGlue Example Applications

  1. 1. 25µm 25µm D A B 25 µm C 100µm F G 100µm H 50µm 100µm E Rapid and Stable 3D Liver Tissue AlbuminSecreon(µg/day/millionhepatocytes) B C Cell Culture A Liver func on assay Drug 0 10 20 30 40 50 60 0 2 4 6 8 10 12 14 Mix 3D Hep 3D Mix 2D Hep 2D Cell Culture Time (Day) Mix 3D Hep 3D Mix 2D Hep 2D Mix 3D Hep 3D Mix 2D Hep 2D Mix 3D Hep 3D Mix 2D Hep 2D 0" 2" 4" 6" 8" 10" 12" 14" 16" CytochromeP4503A4foldacvaon Functional 3D Liver TissueComplex Spheroid Formation Examples of Cell Assembly
  2. 2. Cardiomyocytes Fibroblasts HUVECs Functional 3D Cardiac Tissue with ViaGlue 2D Co-Culture 3D Co-Culture Cardiac Tissue ViaGlue BViaGlue A
  3. 3. 2D Cardiomyocytes 2D Mixed 3D Cardiomyocytes 3D Mixed Fluorescent antibody staining of tissues for expression of cardiac markers (cardiac troponin T and connexin 43). Scale bar: 80µM DAPI cTnT Cxn 43 3D Cardiac Tissue Assembled with ViaGlue
  4. 4. 2D Cardiomyocytes 0.92s 3D Cardiomyocytes 0.64s 2D Mix 0.78s 3D Mix 0.83s Time interval for tissue beats Various Cardiac Tissue Assemblies Spontaneously Beat (no external electrical stimulation) Scaffold free ViaGlue allows high cell contact density for large tissue synchronous beating
  5. 5. A C B D I K L J O P E F M N G H Examples of Various Cell Assemblies Generated by ViaGlue
  6. 6. Generation and analysis of 3-Dimensional Liver Tissue formed via an Inter-cell Bio-orthogonal Mediated Assembly Process. Submitted. A Dual Receptor and Reporter for Multi-Modal Cell Surface Engineering. W.Luo, N. P. Westcott, D. Dutta, A. Pulsipher, D. Rogozhnikov, J. Chen, M.N. Yousaf*. ACS Chemical Biology, 2015, 10, 2219–2226. Spheroid and Tissue Assembly in Microfluidic Flow. P.J. O’Brien, W. Luo, D. Rogoznikov, J. Chen, M.N. Yousaf*. Bioconjugate Chem. 2015, 26, 1939-1949 Remote Control of Tissue Interactions via Engineered Photo-switchable Cell Surfaces. W. Luo, A. Pulsipher, D. Dutta, B. M. Lamb, M. N. Yousaf*. Nature Scientific Reports 2014, 4, 6313. Cell surface engineering by a conjugation and release approach based on the formation and cleavage of oxime linkages upon mild electrochemical oxidation and reduction. A. Pulsipher, D. Dutta, W. Luo, M.N. Yousaf*. Angew. Chem. Int. Ed. 2014, 53, 9487-9492. Engineering Cell Surfaces via Liposome Fusion. D. Dutta, A. Pulsipher, W. Luo, M.N. Yousaf*. Bioconjugate Chemistry. 2011, 22, 2423-2433. Chemical & Engineering News 2011, 49, 36. Synthetic Chemoselective Rewiring of Cell Surfaces: Generation of Three-Dimensional Tissue Structures. D. Dutta, A. Pulsipher, W. Luo, M. N. Yousaf*. J. Am. Chem. Soc. 2011, 133, 8704-8713. Science 2011, 332, 1011-1012.

×