Exercise #testobservationinterpretation of observation

1. EXERCISE #
TEST
OBSERVATION
INTERPRETATION OF OBSERVATION
COLONY MORPHOLOGY
COLONY PIGMENT
GROWTH IN SLANTS
GROWTH IN BROTH
GRAM STAIN
SHAPE
ARRANGEMENT

2. FLUID THIOGLYCOLLATE
GELATIN
PHENOL RED- DEXTROSE
PHENOL RED-SUCROSE
PHENOL RED-LACTOSE
GAS
MR
VP
CATALASE

3. OXIDASE
STARCH AGAR PLATE
UREA
SKIM MILK AGAR PLATE-CASEASE
TRIBUTYRIN AGAR PLATE-LIPASE
CITRATE
PHENYLALANINE
SULFUR
INDOLE

4. MOTILITY
NAMES OF RESEARCHERS IN YOUR GROUP
UNKNOWN #
UNKNOWN IDENTITY
Sheet1Gram Positive ChartB CereusB megateriumB subtilisE
faecalisL lactisM luteusM roseus M phleiS ureaeS aureusS
epidermS pyogenesS salivariusMorphology
rod>3umrodrod>3umcoccuscoccuscoccuscoccusrodcoccuscoccus
coccuscoccuscoccusMorphology
Arrangementpairs,chainspairs,chainspairs,chainspairs,chainspair
s,chainstetrads/clusterstetrads/clustersmycelium-
likepairs,tetradsirreg clustersirreg
clusterspairs,chainspairs,chainsArrangementPigments -----
YellowRed------Pigments Motility +++-----+----Motility
Starch[+]+++--[-]-[-][-]ddStarchCatalase+++d-++++++--
CatalaseOxidase[+]----+d+----OxidaseIndole------------
IndoleVP+-+++dd-++++VPCitrate[+]+[+][-]-+-+-d[-]--
CitrateUrease[-][+]---d-+++-[+]UreaseGelatinase+++d-[+][-]-
+[+]--GelatinaseGlucose+++++[-]+-++++GlucoseLactose-d[-
]++---++++LactoseSucrose+++++[-]+-++++SucroseGas----------
--GasH2S------------
H2SThioglycollate(o/f)Thioglycollate(o/f)gram
stainpurple/bluepurple/bluepurple/bluepurple/bluepurple/bluepu
rple/bluepurple/bluepurple/bluepurple/bluepurple/bluepurple/blu
epurple/bluepurple/bluegram stainMorphology rod bacillusrod
bacillusrod bacillusspherical coccusrod bacillusspherical
coccusspherical coccusspherical coccusspherical
coccusspherical coccusspherical coccusspherical
coccusspherical coccusMorphology arrangementCHAINS,
STREPTOCHAINS, STREPTOCHAINS, STREPTOclusters,

5. staphyloCHAINS,
STREPTOTETRADSTETRADSTETRADSTETRADS,
sarcinaeclusters, staphyloclusters, staphyloCHAINS,
STREPTOCHAINS, STREPTOarrangementpigment-----
Yellowred------pigmentcolony morphologyflat undulate
translucentFLAT, IRREGULARROUND, SMOOTHCONVEX
MUCOIDMUCOID, LOBATEROUND, ENTIRE,SHINY,
CONVEXSMOOTH, CONVEX, ENTIRESMOOTH, CONVEX,
ENTIRERAISED, CIRCULAR, ENTIRERAISED, CIRCULAR,
ENTIRERAISED, CIRCULAR, ENTIRERAISED, CIRCULAR,
ENTIRERAISED, CIRCULAR, ENTIREcolony
morphologygrowth in slantflat undulate translucentFLAT,
IRREGULARROUND, SMOOTHCONVEX MUCOIDMUCOID,
LOBATEROUND, ENTIRE,SHINY, CONVEXSMOOTH,
CONVEX, ENTIRESMOOTH, CONVEX, ENTIRERAISED,
CIRCULAR, ENTIRERAISED, CIRCULAR, ENTIRERAISED,
CIRCULAR, ENTIRERAISED, CIRCULAR, ENTIRERAISED,
CIRCULAR, ENTIREgrowth in slantgrowth in
brothSEDIMENTSEDIMENTSEDIMENTUFTUFTSEDIMENTP
ELLICLEPELLICLEUFTUFTSEDIMENTSEDIMENTUFTgrowt
h in brothsulfur (SIM)-------------sulfur (SIM)indole (SIM)------
-------indole (SIM)motility (SIM)+++--+--+----motility
(SIM)catalase+++±-++++++--catalaseoxidase+----+±±+----
oxidaseMethyl Red (MR)+--+±-±---±--Methyl Red (MR)vogues
proskeur (VP)+-+++-±±-++++vogues proskeur (VP)citrate-+±±-
+-+-±±--citrateUrease-±---+-++++-±UreaseGelatinase-++±--±--
+±--Gelatinasephenyl red dextrose+++++-++-+-++phenyl red
dextrosephenyl red lactose---++----+-++phenyl red
lactosephenyl red sucrose+++++-++-+-++phenyl red sucrosegas-
------------gasfluid
thiolglycollateOAOAOAFAFAOAOAOAOAFAFAFAFAfluid
thiolglycollatePhenylalanine-------+++-+-Phenylalaninestarch
agar plate++++--±++±±±±starch agar plateCasein(skim milk)-
+++±-±+++-+-Casein(skim milk)Lipase (TBA plate)-±±±±-±----
-±Lipase (TBA plate)FAfacultative anaerobeOAobligate
aerobe+90% or greater positive-90% or greater negative±50%

6. POSITIVE 50% NEGATIVEUFTuniform fine turbidity
Sheet2Gram Negative ChartA hydrophilaA faecalisC freundiiE
aerog E cloacaeE coliK pneumP mirabilisP vulgarisP
aeruginosaP fluorescensS marcescensS flexnerigram
stainred/pinkred/pinkred/pinkred/pinkred/pinkred/pinkred/pinkr
ed/pinkred/pinkred/pinkred/pinkred/pinkred/pinkgram
stainMorphology rod bacillusrod bacillusrod bacillusrod
bacillusrod bacillusrod bacillusrod bacillusrod bacillusrod
bacillusrod bacillusrod bacillusrod bacillusrod
bacillusMorphology
arrangementSINGLESINGLESINGLESINGLESINGLESINGLES
INGLESINGLESINGLESINGLESINGLESINGLESINGLEarrang
ementpigment---------blue/greenyellowreddish orange-
pigmentcolony
morphologyFLAT/SMOOTHFLAT/SMOOTHMUCOID,
SMOOTHMUCOID, convexMUCOID, convexFLAT, irregular,
undulate, shinyMUCOID, SMOOTHMUCOID,
SMOOTHMUCOID,
SMOOTHFLAT/SMOOTHFLAT/SMOOTHMUCOID,
convexMUCOID, SMOOTHcolony morphologygrowth in
slantFLAT/SMOOTHFLAT/SMOOTHMUCOID,
SMOOTHMUCOID, convexMUCOID, convexFLAT, irregular,
undulate, shinyMUCOID, SMOOTHMUCOID,
SMOOTHMUCOID,
SMOOTHFLAT/SMOOTHFLAT/SMOOTHMUCOID,
convexMUCOID, SMOOTHgrowth in slantgrowth in
brothSEDIMENTSEDIMENTSEDIMENTSEDIMENTSEDIMEN
TSEDIMENTSEDIMENTSEDIMENTSEDIMENTSEDIMENTSE
DIMENTSEDIMENTSEDIMENTgrowth in brothsulfur (SIM)±-
+----±+----sulfur (SIM)indole (SIM)+----+--+---±indole
(SIM)motility (SIM)++++++-+++++-motility
(SIM)catalase+++++++++++++catalaseoxidase++-+-+---++--
oxidaseMethyl Red±-+-±+±++---+Methyl Redvogues proskeur
(VP)±---±--±---+-citrate±++++-+±±+++-citrateUrease--±-±-
+++±±±-UreaseGelatinase+------+±±±+-Gelatinasephenyl red
dextrose+-+++++++-±++phenyl red dextrosephenyl red

7. lactose±-++±++------phenyl red lactosephenyl red sucrose+-
±++-+-+-±+-phenyl red sucrosegas±-+++++++--±-gasfluid
thiolglycollateFAOAFAFAFAFAFAFAFAOAOAFAFAfluid
thiolglycollatePhenylalaine-------++----Phenylalainestarch agar
plate+----++------starch agar platecasein(skim milk)-------++++-
-casein(skim milk)lipids (TBA plate)---++-++++++-lipids (TBA
plate)FAfacultative anaerobeOAobligate aerobe+90% or greater
positive-90% or greater negative±50% POSITIVE 50%
NEGATIVEUFTuniform fine turbidity
Biochemical and microscopic
analysis of unknown bacteria
EXPERIMENTAL RESULTS
FOR IDENFICIATION OF THE UNKNOWN
BACTERIUM
Microscopic images:
Gram stain, morphology, arrangement
400X magnification
400X magnification
1000X magnification
Cultural Characteristics:
pigment, colony morphology

8. Growth in slant
Growth in broth
SIM test results (sulfur, indole, motility)
Catalase test result
On microscope slide
In tube
Oxidase test result
Methyl Red (MR) test result
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ed+and+voges+proskauer+application&simid=60805486826769
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9. ed+and+voges+proskauer+application&simid=608 05486826769
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Voges-Proskauer (VP) test result
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application&simid=608025585180738723&selectedIndex=0
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OpJNZ&id=AC46E32E3825E673DF38503A6B150A2D69E73A
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vAIogJMYTPDXwEsEs&q=methyl+red+and+voges+proskauer+
application&simid=608025585180738723&selectedIndex=0
Citrate test result
Urease test result
Gelatinase test result
Phenol red dextrose test result
Phenol red lactose test result

10. Phenol red sucrose test result
Phenol red Gas formation test result
Aerotolerance test result:
fluid thiolglycollate (FTG)
Obligate Aerobe (OA) or Facultative Anaerobe (FA)??????
Phenylalanine Deaminase test result
Starch Hydrolysis test result
Casein Hydrolysis test result
Lipid Hydrolysis test result
Biochemical and microscopic analysis of unknown
bacteriaMicroscopic images:�Gram stain, morphology,
arrangementCultural Characteristics:�pigment, colony
morphologyGrowth in slantGrowth in broth SIM test results
(sulfur, indole, motility)�Catalase test resultOxidase test
resultMethyl Red (MR) test resultVoges-Proskauer (VP) test

11. resultCitrate test result�Urease test resultGelatinase test
resultPhenol red dextrose test resultPhenol red lactose test
resultPhenol red sucrose test resultPhenol red Gas formation
test resultAerotolerance test result:�fluid thiolglycollate
(FTG)Phenylalanine Deaminase test resultStarch Hydrolysis test
resultCasein Hydrolysis test resultLipid Hydrolysis test result�