Supernova for CALI technique
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Based on the research of Takemoto et al, Supernova is a new fluorescent protein for CALI microscopy technique
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Supernova for CALI technique
- 1. SUPERNOVA A MONOMERIC PHOTOSENSITIZING FLUORESCENT PROTEIN FOR CHROMOPHORE-ASSISTED LIGHT INACTIVATION KIWAMUTAKEMOTO ET AL. 2013 SCIENTIFIC REPORTS
- 2. PHOTOSENSITIZERS Bulina et al., 2006
- 3. CALI CHROMOPHORE-ASSISTED LIGHT INACTIVATION Photosensitizer Protein Target Protein intense light irradiation Target inactivation Cellular Death
- 4. PHOTOSENSITIZERS Li et al., 2012
- 5. KILLER REDVS SUPER NOVA Takemoto et al., 2013, Bulina et al., 2006 579 nm 610 nm
- 6. SuperNova KillerRed EGFP Cx43 Keratin Fibrillarin KILLER REDVS SUPER NOVA Scale bar 10µm Takemoto et al., 2013,
- 7. EXPERIMENTAL RESULTS 0 15 30 45 60 75 90 Takemoto et al., 2013, Bulina et al., 2006 Magnification 100x
- 8. EXPERIMENTAL RESULTS Actin-GFP Cofilin-SuperNova Before CALI After CALI Takemoto et al., 2013 Scale bar 10µm
- 9. CALI APPLICATIONS Metaphase Early Cytokinesis Late Cytokinesis Death Survival Ou et al., 2012
- 10. CALI IN C.ELEGANS Scale bar 5µm Scale bar 2,5µm WT CALI QR.a QR.a (pig-1) QR.p
- 11. REFERENCES Wei Li, Nico Stuurman, Guangshuo Ou. (2012). Chromophore-assisted laser inactivation in neural development. Neuroscience Bulletin. 28 (4), 333–341. Maria E Bulina, Konstantin A Lukyanov, Olga V Britanova, Daria Onichtchouk, Sergey Lukyanov & Dmitriy M Chudakov. (2006). Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nature Protocols. 1 (2), 947-953. Maria E Bulina, Dmitriy M Chudakov, Olga V Britanova, Yurii G Yanushevich, Dmitry B Staroverov, Tatyana V Chepurnykh, Ekaterina M Merzlyak, Maria A Shkrob Sergey Lukyanov & Konstantin. (2006). A genetically encoded photosensitizer. Nature Biotechnology. 24 (1), 95-99. Guangshuo Ou, Nico Stuurman, Michael D’Ambrosio, Ronald D. Vale. (2010). Polarized Myosin Produces Unequal-Size Daughters During Asymmetric Cell Division. Science. 330, 677-679. Takemoto, K., Matsuda, T., Sakai, N., Fu, D., Noda, M., Uchiyama, S., ... & Nagai, T. (2013). SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Scientific reports, 3. Look for diagnosis.com (2014). Chromophore-Assisted Light Inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Available: http://jcs.biologists.org/content/127/8/1621/F1.large.jpg.