6. ASEPSIS
Asepsis is a state of being free from disease –
causing microbes such as bacteria, viruses,
fungi and parasites.
7. PARTS OF ASEPSIS
Practices that prevent entry of microbes into
the nursery environment
Practices that prevent proliferation of microbes
in nursery
Practices that prevent spread of microbes
between babies
Practices that protect newborn from
developing infections
Practices that enable better asepsis and
administration
12. Contd..
HANDWASHING
Before first entry into the nursery, full surgical
scrub upto elbows for a min of 2- 3 min with
appropriate antimicrobial scrub
Washed between each infant contact (15-20
sec)
Jewellery must be removed
14. VISITORS
Same as non –nursery staff
Visitors fills out a health screen
Must be symptom free
15. NURSERY ATTIRE
Standards uniform or scrubs
Persons entering must remove outer coats and
jackets and perform scrub
Non –nursery personnel must wear a gown
16. Contd..
PERSONNEL
Prior to employment,nurses will complete a
pre-placement physical by Hospital Health
Service
Any staff member with a known contagious
illness is not permitted in NICU
18. HOUSEKEEPING ROUTINES
Avoid dry dusting and sweeping instead use
vacuum cleaners
Wet mopping with soap and water (3% phenol)
- 3 times/day
19. DAILY
Incubators, warmers, syringe
pump, infusion pumps,
phototherapy units, Mattress, Pulse
oximeter, multichannel monitors,
Oxygen hood, Ventilator, CPAP
machine, Telephone
Dry dusting, clean using a moist
wipe
Suction bottles, Humidifier
chamber, water in Bubble CPAP
Change with distilled water
Ventilator filters Clean daily and dust off
Bag and mask
Immerse in 2% cidex for 6 to 8
hours after dismantling and
cleaning with running water.
20. DAILY
Incubators / Radiant warmers
Clean with 2% Bacillocid if not
occupied by an infant.
Laryngoscopes, masks,
stethoscopes, measuring tapes,
Thermometer, BP cuff, temperature
and Spo2 probes, Torches
Wipe with spirit
Walls, floor, Wash basins
Clean with polysan or phenol or
Lysol or 2% bacillocid or 0.5%
chlorine (for walls only) in each shift
Dust bins, buckets, waste
Empty during each shift and clean
with soap and water
21. WEEKLY
Ventilator and CPAP circuits Change with a new circuit
Procedure sets
Autoclave after every use and keep
ready the sets
Window Air-conditioners
Surfaces and filters with soap and
water
Refrigerators
Sorted and cleaned separate fridge
for milk and lab samples
Thermometer, weighing scale, stethoscopes, BP cuff,
laryngoscopes should be cleaned and wiped with spirit after
every use. Feeding utensils/ paladai should be boiled for 15
minutes after cleaning after each use.
22. WASTE DISPOSAL
Black drums (waste disposal by
dumping
Left-over food, vegetables, waste
paper, packing material, empty bags
etc
Yellow drums (disposed by
incineration)
Infected non plastic waste- human
secreta, blood and body fluids.
Blue drums (made noninfectious by
autoclaving and disposed by
shredding)
Infected plastic waste like IV sets,
ET tubes, catheters, urobags etc
23. INFECTION CONTROL STEP
3
PREVENTING INFECTION SPREAD FROM
PROLIFERATION SITES TO BABY AND
FROM ONE BABY TO OTHER
24. CONTD..
Nurse to patient ratio
Use disposables
Laminar flow system for drugs , fluids and
TPN preparation
33. INFECTION CONTROL
COMMITTEE
1. Yearly programme of activity for surveillance
& prevention
2. Epidemiological surveillance data and identify
areas for intervention
3. Ensure appropriate staff training in infection
control and safety
4. Provide inputs into investigation of epidemics
34. CARE OF INFANTS AND
EQUIPMENTS
Infants placed in isolated incubator to prevent
cross infection
Strict handwashing
Proper disposal of linen and other
contaminated articles
Proper disinfection
42. DISINFECTIONS AND
GERMICIDES
1. BACILLOCID 100 g-
Formaldehyde, glutaraldehyde, alkylurea
derivative, benzalkonium chlorides
Use 2% solution (200 ml in 10 L water) for
cleaning surface and spraying
30 mts for good efficacy
Avoid contact with concentrated
solution
5 ml=1 sqm
43. KORSOLEX 100 g
Formaldehyde, glutaraldehyde, polymethylol
urea formaldehyde
I :9 to get 10% solution
20 min for sterilization
4 hrs for disinfection
Active for 14 days
48. FORMALIN
Formaldehyde aqueous solution
Fumigation-routine (30 ml of 4% in 90 ml of
water)
Intensive(90 ml of 4% in 90 ml)
OTICARE-30 min , room closed for 6 hrs
49. DISINFECTION OF
EQUIPMENT
1. RESUSCITATION EQUIPMENT-
Bags and masks-cidex
Silicon bags and masks-autoclaving
Laryngoscope-70% isopropyl alcohol
Alcohol should not be used for cleaning as it
causes opacification
50. Contd..
2.OPEN CARE SYSTEMS AND COTS
Cleaned everyday with 3% phenol or 5% lysol
Mattresses plastic cover should be intact to
clean with antiseptic solution
Blankets for autoclaving
Laminar air flow for drugs , fluids ,TPN
51. Contd..
3.INCUBATORS
Thoroughly disinfected every 7th day
After discharge or death -50 ml formalin in 50
ml water
Canopy and mattress should be cleaned with
soap and water
Humidification unit –disinfected with cidex for
10 min
53. Contd..
6.FEEDING UTENSILS
Bottles and teats- wash with soap and water
after feeds and then boiled or autoclaved
Spoons and paladays –bolied for 15 min
before use