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Manufacturing functional tissues in vitro using bioprinting and bioreactors
- 1. Manufacturing of functional tissues in vitro using bioprinting and bioreactors M.Sc. José Manuel Baena CEO BRECA Health Care/REGEMAT3D Research associate Group Advanced therapies, CIBM, UGR, Granada, Spain Tissue Engineering and 3D Printing Platform HULP, Madrid @Josbaema Jm.baena@brecahealthcare.com CEO@regemat3D.com
- 2. www.brecahealthcare.com www.regemat3d.com BRECAHC /REGEMAT Custom made synthetic Medical devices. Reconstructions, promote the new tissue formation. Design, simulation and manufacturing
- 3. www.brecahealthcare.com www.regemat3d.com BRECAHC /REGEMAT Tissue construct Scaffold Cell laden bioink Bio molecules Bioprinting process adding the different ingredients of the 3D construct Maturation of the 3D construct in a bioreactor Mechanical stimuli, controlled conditions….. Biofabrication systems and bioreactors Ingredients + process to generate a functional tissue
- 4. Traditional manufacturing techniques are based on the subtraction of material from a raw block or on modifying the shape by means of an external power source and shaping it using a mold. 3D printing www.brecahealthcare.com www.regemat3d.com
- 5. 3D printing 3D printing (3DP), AM, rapid manufacturing….are based on the principle of adding material layer by layer allowing the manufacture of complex external and internal shapes with a mesh structure (scaffold). www.brecahealthcare.com www.regemat3d.com
- 6. Custom made medical devices www.brecahealthcare.com www.regemat3d.com “We fit the product to the patient not the patient to the product”
- 12. Neurosurgery Virtual surgery planning Craniosynostosis is a condition in which one or more craneal sutures close prematurely, impeding the normal development of the brain and changing the normal bone grow pattern. As a result patient that suffer from this condition have large bone defects. www.brecahealthcare.com www.regemat3d.com
- 13. Neurosurgery Virtual surgery planning Surgical guides for Craneosynostosis www.brecahealthcare.com www.regemat3d.com
- 14. Neurosurgery Virtual surgery planning Surgical guides for Craniosynostosis www.brecahealthcare.com www.regemat3d.com
- 25. www.regemat3d.com But we are not going to implant metals forever… Our mission is to develop innovative solutions in the area of bioprinting and regenerative medicine towards the clinical application of this amazing technology, aiming at improving people´s quality of life
- 26. Bioprinting (BP)
- 27. And what can we do using BP? Print 3D multi component parts with different materials with a customized external shape and an internal mesh structure that mimic human living tissues. Cell-laden Hydrogel Growth factorsScaffold
- 28. REGEMAT 3D
- 29. REGEMAT 3D UNIQUE CUSTOM MADE SYSTEMS Bioprinters and Bioreactors
- 30. REGEMAT 3D
- 31. Tissue construct Scaffold Cell laden bioink Bio molecules Bioprinting process adding the different ingredients of the 3D construct Maturation of the 3D construct in a bioreactor Mechanical stimuli, controlled conditions….. Biofabrication
- 32. Validation of the work station We used Infrapatellar fat pad (Hoffa´s) and chondrocites as described in Lopez Ruiz et al., 2013 and induced chondrogenic differentiation of autologous mesenchymal stem cells (MSCs).
- 33. Validation of the work station We had some interesting results Validated with PLA, alginate and chondrocytes for cartilage regeneration
- 34. Comparative results between the use of PLA/Alginate and the addition of Hyaluronic Acid PLA /Alginate vs PLA /Alginate /HA
- 35. IVF
- 36. IVF (a) Characterization of freshly isolated chondrocytes that displayed a typical polygonal shape, high expression of collagen type 2 (Col2) and proteoglycans. Original magnification: 10X. (b) Cell viability values of chondrocytes manually printed with alginate (ALG) and printed with alginate and alginate in combination with PLA using Bioprinter REGEMAT 3D V1. *p<0.05 compared with control cells before printing process. (c) Cell proliferation using Alamar blue assay at different time points.
- 37. IVF (*) Confocal laser scanning microscopy images of bioprinted human chondrocytes stained with CTG (green) and DAPI (blue) after cultured for 24 hours and 7 days. Scale bars indicate 100 µm.
- 38. Bioreactors
- 39. Clinical trial development Contour guide
- 40. From the segmentation to the printing procedure
- 41. From the segmentation to the printing procedure
- 42. From the segmentation to the printing procedure
- 43. From the segmentation to the printing procedure
- 44. From the segmentation to the printing procedure
- 45. From the segmentation to the printing procedure
- 46. From the segmentation to the printing procedure
- 47. From the segmentation to the printing procedure
- 50. We adapt our technology to the specifications of our customers Custom made systems...
- 51. Some results and publications
- 52. Some results and publications
- 53. Some results and publications
- 54. New procedures towards the clinical application
- 55. First bioprinting PhD thesis in Spain
- 57. Applications
- 58. Applications
- 59. Applications
- 60. Applications
- 61. Applications
- 62. Applications
- 63. Direct VS Indirect fabrication Biofabrication
- 68. Video
- 72. HONORS AND AWARDS AND MEDIA
- 73. See you in Granada @Josbaema Jm.baena@brecahealthcare.com CEO@regemat3D.com