Protein Fraction & Quantification.pptx
- 1. Protein Fraction & Quantification
Ion exchange
SDS-PAGE
AKINSEHINWA SAMUEL – 111726107
JAWHER ALI – 111726108
LUO YUN JIANG-112333101
- 2. Protein Fraction & Quantification
Concentration (BSA mg/ml) Absorbance 1 (OD595) Absorbance 2 (OD595) Avg Absorbance (OD595)
0.02 0.05 0.048 0.049
0.04 0.08 0.087 0.0835
0.08 0.151 0.154 0.1525
0.12 0.192 0.234 0.213
0.2 0.362 0.351 0.3565
2.50% 0.309 0.262 0.2855
5.00% 0.408 0.461 0.4345
- 4. Ion exchange
Tube number Absorbance (OD280nm) NaCl (M)
1 0.196 0
2 0.663 0
3 0.629 0
4 0.246 0.1
5 0.939 0.1
6 0.257 0.1
7 0.322 0.3
8 0.493 0.3
9 0.45 0.3
10 0.422 0.5
11 0.098 0.5
12 0.054 0.5
Editor's Notes
- Ovalbumin:42.7kDa (PI:4.7) < PH:7 → Negative
Lysozyme:14.4kDa (PI:11.1) >PH:7 → Positive