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2. Noise type and intensity differentially affect hearing thresholds, with both noise treatments causing a greater masking effect in detection of a tone burst sound
3. White noise at 170dB re 1µPa completely masked detection of a tone burst sound
4. Information on hearing thresholds obtained through this study will help with fine tuning sounds that can be incorporated within acoustic traps to attract and capture round gobiesShips from Black and Caspian Seas Invasion of the Great Lakes Study organism Decline in native fish populations Fig.1 Auditory brainstem response setup. Speaker A played either a tone burst or conspecific call whilst speaker B played background or white noise. Red and black line illustrate recording and reference electrodes, respectively. Hearing threshold determination 1Amoser, S. and Ladich, F. (2005). Are hearing sensitivities of freshwater fish adapted to the ambient noise in their habitats? J. Expt. Biol. 208, 3533 – 3542. 2Tavolga, W.N. (1967).Masked hearing thresholds in teleost fishes. In Marine Bioacoustics, Vol. 2 (ed. W.N. Tavolga). Pergamon Press, Oxford, 233 – 245 pp. 3Popper, A.N. and Hastings, M.C. (2009). The effects of human – generated sound on fish. Int. Zool. 4, 43 – 52. 4Lugli, M., Yan, H.Y. and Fine, M.L. (2003). Acoustic communication in two freshwater gobies: the relationship between ambient noise, hearing thresholds and sound spectrum. The Journal of Comparative Physiology 189, 309 – 320. 5Yost, W.A. (2000). Fundamentals of hearing. Academic Press, New York. Hearing threshold was defined as the lowest sound pressure level (SPL) for which a repeatable ABR trace was observed, as determined by visual inspection of overlaying traces4,5. Because of their negative impact on the local fauna, our long term goal is to prevent this species’ rapid invasion by building acoustic traps. Fig.3 Audiograms indicate that 200Hz was the frequency most affected by noise. Summary of Results Background noise White noise Background noise White noise Experimental Design Conclusions References Brainstem recording 135dB 150dB 162dB 162dB 150dB 162dB 170dB I wish to thank Dr. Higgs for all his help and support with this project, Dr. Poling, Dr. Zielinski for providing experimental animals, as well as all the members of the Higgs lab for their advice and comments . 162dB 170dB Tone burst Goby call Fig.2 ABR trace obtained in response to a tone burst sound at 200Hz. Region enclosed within the arrows underlines the brainstem response, increasing in amplitude with increasing SPL. The box shows the hearing threshold. Results Fig.4 Differences in hearing thresholds between baseline ( no noise) audiogram for each test condition and masked audiograms. High thresholds shift was caused by background noise at 162dB re 1µPa with respect to tone burst detection, whilst no significant difference in the shifts observed with respect to goby call detection. The following represent audiograms displaying hearing thresholds measured in response to either a tone burst sound or conspecific call. Thresholds are displayed first in the absence of noise, and then in the presence of various noise conditions. Acknowledgements All values are in dB re 1µPa