can Fluorescence Resonance Emission Transfer (FRET) Microscopy be used to image static structures?? Solution The technique of fluorescence resonance energy transfer , when applied to optical microscopy, permits determination of the approach between two molecules within several nanometers.FRET is a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution. FRET microscopy relies on the ability to capture fluorescent signals from the interactions of labeled molecules in single living or fixed cells. Hence FRET Microscopy is used to image static structures..