(28pts.) The student wants to determine if the TH1 cells that she isolated by FACS specifically recognize MCMV ens, and induce B cells to generate antibodies against MCMV proteins. She uses a panel of MCM peptide epitopes, presented on MHCll by DC in culture, and finds that a subpopulation of the T cells proliferate and release a cytokine that indicates activation. Solution 1) Cytokine : Interleukin 12. Dendritic cells (DCs) produce cytokines during murine cytomegalovirus (MCMV) infection to polarize naive T cells into TH1 cells. Interferon alpha/beta and interleukin 12 are common cytokines produced in response to viral infections. 2) Fluorescence-activated cell sorting (FACS) can be used to detect the B cells expressing specific type of antibody. Because TH1 cells induced B cell proliferation expresses only specific type of antibody on its surface. Based on this expression, cells can be sorted..

1. (28pts.) The student wants to determine if the TH1 cells that she isolated by FACS specifically
recognize MCMV ens, and induce B cells to generate antibodies against MCMV proteins. She
uses a panel of MCM peptide epitopes, presented on MHCll by DC in culture, and finds that a
subpopulation of the T cells proliferate and release a cytokine that indicates activation.
Solution
1) Cytokine : Interleukin 12. Dendritic cells (DCs) produce cytokines during murine
cytomegalovirus (MCMV) infection to polarize naive T cells into TH1 cells. Interferon
alpha/beta and interleukin 12 are common cytokines produced in response to viral infections.
2) Fluorescence-activated cell sorting (FACS) can be used to detect the B cells expressing
specific type of antibody. Because TH1 cells induced B cell proliferation expresses only specific
type of antibody on its surface. Based on this expression, cells can be sorted.