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A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of a
pBR322 vector. The vector also contains a TetR gene that is not interrupted. After introducing
the recombinant DNA into bacterial cells, the resulting transformants were tested on a variety of
media. Which of the following results would be expected?A)no growth on either ampicillin or
tetracycline containing mediaB)growth on ampicillin but not tetracycline containing
mediaC)growth on tetracycline but not ampicillin containing mediaD)growth only on media
containing both antibioticsE)growth on both ampicillin containing media and tetracycline
containing media
Solution
The following results would be expected:
B)
growth on ampicillin but not tetracycline containing media
As due to insertional inactivation growth will be seen in case of ampicillin but not in case of
tetracycline containing media as the TetR gene that is not interrupted.
B)
growth on ampicillin but not tetracycline containing media

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  • 1. A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of a pBR322 vector. The vector also contains a TetR gene that is not interrupted. After introducing the recombinant DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected?A)no growth on either ampicillin or tetracycline containing mediaB)growth on ampicillin but not tetracycline containing mediaC)growth on tetracycline but not ampicillin containing mediaD)growth only on media containing both antibioticsE)growth on both ampicillin containing media and tetracycline containing media Solution The following results would be expected: B) growth on ampicillin but not tetracycline containing media As due to insertional inactivation growth will be seen in case of ampicillin but not in case of tetracycline containing media as the TetR gene that is not interrupted. B) growth on ampicillin but not tetracycline containing media