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International Journal of Forest, Animal and Fisheries Research (IJFAF)
ISSN: 2456-8791
[Vol-5, Issue-6, Nov-Dec, 2021]
Issue DOI: https://dx.doi.org/10.22161/ijfaf.5.6
Article DOI: https://dx.doi.org/10.22161/ijfaf.5.6.2
Int. J. Forest Animal Fish. Res.
www.aipublications.com/ijfaf Page | 4
Effects of Cinnamon Powder on Egg Quality: A New
Approach using Layer Birds
Usman Sulaiman, Odewamide Lateef Adedokun
Faculty of Agricultural Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
Received: 03 Oct 2020; Received in revised form: 03 Nov 2021; Accepted: 18 Nov 2021; Available online: 25 Nov 2021
©2021 The Author(s). Published by AI Publications. This is an open access article under the CC BY license
(https://creativecommons.org/licenses/by/4.0/)
Abstract— Attention towards natural growth promoters in poultry has been on the increase in different parts
of the world. However, most studies focus on broiler birds and quail. This study analyzed how cinnamon
powder supplemented in the diet of Lohmann Brown layer birds can improve egg quality. This study consisted
of 5 treatments of 30 birds per treatment, comprising fifteen replicates of 2 birds per replicates. The
treatments included: T1: Control without Cinnamon; T2: 4 litres of water + 0.1g of cinnamon; T3: 4 litres
of water + 0.2g of cinnamon; T4: 4 litres of water + 0.3g of cinnamon; T5: 4 litres of water + 0.4g of
cinnamon. Parameters measured were egg weight, egg mass, egg breadth, egg length, albumen height,
albumen weight, yolk height, yolk length, yolk colour, yolk weight, shell weight, and shell thickness. The
results revealed an increase in the albumen weight, yolk weight, albumen height, and yolk height as the
inclusion levels of cinnamon powder increase. In addition, the egg weight was highest in treatment 5. Taken
together, cinnamon powder addition in the diet of Lohmann Brown layer birds has a positive outcome on
egg quality.
Keywords— Antibiotic growth promoters, Cinnamon, Egg quality, Layers, Poultry
I. INTRODUCTION
Poultry production has been on the increase over the years
in different parts of the world. According to research, it is
the most consumed meat globally, with production peaking
137 million tons in 2020 (Ali et al., 2021). Even in
developing countries like Nigeria, poultry is the fastest
growing subsector of animal husbandry, with eggs being the
most affordable and demanded product (Heise et al., 2015).
In order to keep up with demand, antibiotic growth
promoters (AGP) have long been in use as the primary
growth promotant and carcass modifier. However, due to
increasing consumer awareness about food safety, AGPs
have been banned in most countries of the world (Beg et al.,
2016).
The global trend against antibiotic growth promoters in
animal diets has led researchers to find safer and natural
alternatives. Hence, natural products like rosemary,
turmeric, cumin, and cinnamon have been in the spotlight in
the past decade due to their natural medicinal properties
(Rao & Gan, 2014).
Cinnamon belonging to the genus Cinnamomum is the most
consumed spice globally, with over 250 evergreen trees and
shrubs mainly found in Asia and Australia. It has been in
use in different countries for thousands of years with
reference in ancient scriptures (Barceloux, 2009). As yet,
research have been carried out to evaluate the effects of
Cinnamon on broiler meat and quail eggs (Ciftci et al.,
2010; Santos et al., 2019).
This paper aimed to explore the effects of Cinnamon
powder on egg quality parameters of commercial laying
birds fed varying levels of cinnamon powder inclusion in
their diets.
II. MATERIALS AND METHODS
The experiment was carried out using battery cages at the
Poultry Unit of the Teaching and Research Farm at Ladoke
Akintola University of Technology Ogbomoso, Oyo state.
One hundred and fifty (150) point of lay birds (Lohmann
Brown) were used for this experiment, and the birds were
Usman Sulaiman et al. International Journal of Forest, Animal and Fisheries Research (IJFAF)
5(6)-2021
Int. J. Forest Animal Fish. Res.
www.aipublications.com/ijfaf Page | 5
purchased at 18 weeks old from Global West Farms at
Ewura in Oyo State. Before starting the experiment, the
birds were allowed to acclimatize for three weeks and
randomly divided into five treatments of 30 birds per
treatment comprising fifteen replicates of 2 birds per
replicates. They were offered adequate feed, clean and fresh
water daily throughout the experiment and were submitted
to a light regimen of 15 hours daily. Diet was fed to each
treatment group at 7.00 am and 12.00 pm daily. The
experiment started when the birds have started laying fully
at 21 weeks old and lasted till 33 weeks old translating to
twelve (12) weeks of data collection.
The Cinnamon powder (Cinnamomum zeylanicum) used
was procured from Gel Spice Company in its finished fine
powder form. Layer mash and Cinnamomum zeylanicum
was introduced to the commercial layers when they started
laying at an inclusion level of 4litres of water in 0g, 0.1g,
0.2g, 0.3g and 0.4g of cinnamon powder. Cinnamon powder
was adequately mixed to dissolve in drinking water fully to
avoid settling of the powder. Five experimental diets
(Cinnamomum zeylanicum + 4 litres of water) was
formulated:
Diet 1: Control without Cinnamon, Diet 2: 4 litres of water
+ 0.1g of cinnamon, Diet 3: 4 litres of water + 0.2g of
cinnamon, Diet 4: 4 litres of water + 0.3g of cinnamon, and
Diet 5: 4 litres of water + 0.4g of cinnamon.
Eggs were collected and weighed from each replicate daily.
At the end of each week, one egg was randomly selected
from each replicate. It was subjected to the following
measurements: Egg weight (g), egg mass (g), egg breadth
(mm), egg length (mm), albumen height (cm), albumen
weight (g), yolk height (cm), yolk length (mm), yolk colour,
yolk weight (g), shell weight (g), shell thickness (mm).
Weight measurements were carried out using an electric
weighing balance. Length and breadth measurements using
a vernier calliper. Height was measured using a ruler while
the thickness with a micrometre screw gauge. Yolk colours
were measured by comparing with an egg yolk colour fan.
Data obtained from this study were subjected to analysis of
variance using SAS. Significantly different means were
separated using Duncan’s Multiple Range Test (DMRT) of
the same package.
*Premix composition per kg diet: Vitamin A 10,000,000iu, Vitamin B 13750 Mg, Vitamin B2 5000Mg, Vitamin B6 3750Mg,
Vitamin B12 2000 Mg, Vitamin D3 2,500,000iu, Vitamin E30,000iu, Vitamin k3 5000Mg, Antioxidant 1250 Mg, Biotin 1500
Mg, Choline chloride 500,000Mg, Copper 6500 Mg, Cobalt 200 Mg, Folic 125 Mg, Iron 70,200 Mg, Iodine 400 Mg, manganese
75,000 Mg, Niacin (PP) 30,000Mg, Selenium 80 Mg, Zinc 85,500Mg.
Table 1: Gross composition and nutrient content of experimental diets
Feed Ingredients Quantity (%)
Maize 35.00
Groundnut Cake 12.000
Soybean Meal 9.000
Palm Kernel Cake 15.000
Corn Bran 15.00
Fish Meal 1.200
Oyster Shell 8.000
Bone Meal 4.000
Lysine 0.150
Methionine 0.200
Premix* 0.250
Salt 0.200
TOTAL 100.000
Crude Protein (g kg-1
) 17.890
ME (Kcal kg-1
) 2497.270
Crude Fiber (%) 5.347
Calcium (g kg-1
) 29.22
Available Phosphorus (g kg-1
) 3.04
Sodium (g kg-1
) 1.46
Chlorine (g kg-1
) 2.42
Potassium (g kg-1
) 7.25
Usman Sulaiman et al. International Journal of Forest, Animal and Fisheries Research (IJFAF)
5(6)-2021
Int. J. Forest Animal Fish. Res.
www.aipublications.com/ijfaf Page | 6
III. RESULTS
The results of the supplementation of cinnamon powder on
external egg quality characteristics of layers is shown in
table 2. There were significant (p<0.05) differences among
the parameters measured across the treatment except for egg
length, similar all through. The highest value of the egg
weight was recorded in T5 (61.32g), with the lowest value
in T2 (58.24g).
Egg mass was highest in T5 (54.08g), similar to T3 (53.47g),
while the lowest value of 51.31g was observed in T2, similar
to T1 with the value 51.87g. The birds on T5 have the highest
egg breath value, 43.63g, while T1, T3 and T4 were observed
to be similar with the values of T1 (43.30g), T3 (43.57g) and
T4 (43.40g) with the lowest value (43.04g) recorded in T2.
Table 2: Mean values of cinnamon powder on external egg quality characteristics of layers chicken
Parameters T1 T2 T3 T4 T5 ±SEM
Shell weight 4.63ab
4.48ab
4.39b
4.78a
4.48ab
0.47
Shell thickness 0.33b
0.32b
0.50a
0.34b
0.33b
0.13
Egg weight 59.22bc
58.24c
60.34ab
59.65abc
61.32a
2.43
Egg mass 51.87b
51.31b
53.47a
52.62ab
54.08a
2.24
Egg length 55.16a
55.43a
54.82a
55.64a
55.50a
1.67
Egg breadth 43.30ab
43.04b
43.57ab
43.40ab
43.63a
0.81
T1: Control without Cinnamon; T2: 4 litres of water + 0.1g of cinnamon; T3: 4 litres of water + 0.2g of cinnamon; T4: 4 litres
of water + 0.3g of cinnamon; T5: 4 litres of water + 0.4g of cinnamon. Means along the same row with different superscripts
are significantly (p<0.05) different, SEM=Standard Error Mean.
Table 3 shows the mean values of cinnamon powder on
internal egg quality characteristics of layers. There were
significant (p<0.05) differences among the parameters
measured across the dietary treatments. The value of the
albumin weight was observed to be highest in T5 (38.95g),
the lowest value was in T2 (36.64g).
Yolk height was highest in T5 (2.24g), while the lowest was
observed in chickens fed on diet T2 (2.07g). The result
revealed an increase in the albumen weight, yolk weight,
albumen height, and yolk height as the inclusion levels of
cinnamon powder increase.
Table 3: Mean values of cinnamon on internal egg quality characteristics of layers
T1: Control without Cinnamon; T2: 4 litres of water + 0.1g of cinnamon; T3: 4 litres of water + 0.2g of cinnamon; T4: 4 litres
of water + 0.3g of cinnamon; T5: 4 litres of water + 0.4g of cinnamon. Means along the same row with different superscripts
are significantly (p<0.05) different, SEM=Standard Error Mean.
Parameters T1 T2 T3 T4 T5 ±SEM
Albumen weight 36.80b
36.64b
37.80ab
37.61ab
38.95a
2.14
Yolk weight 14.38a
13.93b
14.39a
13.93b
14.53a
0.15
Albumen height 1.62a
1.61a
1.63a
1.78a
1.67a
0.27
Yolk length 37.05a
36.90a
37.20a
37.04a
37.01a
1.42
Yolk height 2.11b
2.07b
2.10b
2.11b
2.24a
0.12
Yolk colour 7.45a
6.85b
7.20a
6.92a
6.63a
1.38
Usman Sulaiman et al. International Journal of Forest, Animal and Fisheries Research (IJFAF)
5(6)-2021
Int. J. Forest Animal Fish. Res.
www.aipublications.com/ijfaf Page | 7
IV. DISCUSSION
In the present article, we investigated whether cinnamon
affects the egg qualities of laying commercial birds. Our
findings converge with Suwarta & Suryani (2019) research
on quail that cinnamon powder significantly increased egg
weight and egg shell weight. In addition, our study revealed
for the first time that shell weight, shell thickness,
egg weight, egg mass and egg breadth were significantly
influenced by cinnamon powder supplementation in the diet
of layer birds. Vali et al. (2013) reported that
supplementation of Cinnamon zeylanicum bloom and
Thymus vulgaris on the egg quality of Japanese quail
significantly improved egg quality parameters, supporting
the present result on egg quality and cinnamon powder.
Ulku et al. (2015) on the effect of cinnamon and rosemary
oils on egg quality in laying quails indicated that cinnamon
oil added a positive effect on egg shell quality.
Our finding revealed a significant interaction between
cinnamon powder and egg weight, in line with Torki et al.
(2015) and Hesham et al. (2019), who studied the effect of
cinnamon essential oil on the egg quality of laying hens.
Abo Ghanima et al. (2020) reported on the effect of housing
system, rosemary and cinnamon essential oils on egg
quality traits, reviewed that supplementation of rosemary
and cinnamon essential oils in laying hens had a significant
effect on egg quality characteristics. The study of Vali &
Mottaghi (2016) reported the effect of using different levels
of cinnamon and thyme powder on egg quality
characteristics in Japanese quails reviewed that egg shell
and egg shell thickness increases significantly compared to
control.
However, the result of this study contradicted the findings
of Santos et al. (2019), who reported that the use of
cinnamon in Japanese laying quail had no significant effect
on performance or the egg quality variable. Except for the
specific gravity and colour of the yolk, which increases
linearly as the inclusion of cinnamon powder increases. The
findings of Soomro et al. (2018) also disagreed with the
current study that the alternative use of turmeric in induced
moulting on the performance of layers had non-significant
differences among the groups for egg width, yolk weight,
and albumin weight.
V. CONCLUSION
In conclusion, the result of the study indicated that the
inclusion of cinnamon powder at 0.4g/4liters to the drinking
water of layers has improved egg quality compared to other
dietary treatments. Thus, cinnamon can be used as a natural
egg booster for layer birds. Future research may extend this
work by exploring other natural AGP alternatives on
different breeds of laying birds.
CONFLICT OF INTEREST
The authors declare that there is no conflict of interest.
ETHICAL APPROVAL
The two authors have declared that, “principle of laboratory
animal care” (NIH publication 8023 revised 1978) were
followed as well as the University law.
AUTHORS' CONTRIBUTIONS
This work was conducted in collaboration by both authors.
Both authors have read and approved the final version of the
manuscript.
REFERENCES
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[3] Barceloux, D. G. (2009). Cinnamon (Cinnamomum
Species). Disease-a-Month, 55(6), 327–335.
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[6] Heise, H., Crisan, A., & Theuvsen, L. (2015). The poultry
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[7] Hesham Mahmoud Azouz, S. S. Gadelrab , M. M. Beshara,
Z. M. D. and S. A. S., & Anim. (2019). EFFECTS OF
DIETARY TURMERIC AND HOT PEPPER POWDER.
Egyptian Poultry Science Journal, 39(4), 935–951.
[8] Rao, P. V., & Gan, S. H. (2014). Cinnamon: A multifaceted
medicinal plant. Evidence-Based Complementary and
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www.aipublications.com/ijfaf Page | 8
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Cinnamon Improves Egg Quality in Laying Hens

  • 1. International Journal of Forest, Animal and Fisheries Research (IJFAF) ISSN: 2456-8791 [Vol-5, Issue-6, Nov-Dec, 2021] Issue DOI: https://dx.doi.org/10.22161/ijfaf.5.6 Article DOI: https://dx.doi.org/10.22161/ijfaf.5.6.2 Int. J. Forest Animal Fish. Res. www.aipublications.com/ijfaf Page | 4 Effects of Cinnamon Powder on Egg Quality: A New Approach using Layer Birds Usman Sulaiman, Odewamide Lateef Adedokun Faculty of Agricultural Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria Received: 03 Oct 2020; Received in revised form: 03 Nov 2021; Accepted: 18 Nov 2021; Available online: 25 Nov 2021 ©2021 The Author(s). Published by AI Publications. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/) Abstract— Attention towards natural growth promoters in poultry has been on the increase in different parts of the world. However, most studies focus on broiler birds and quail. This study analyzed how cinnamon powder supplemented in the diet of Lohmann Brown layer birds can improve egg quality. This study consisted of 5 treatments of 30 birds per treatment, comprising fifteen replicates of 2 birds per replicates. The treatments included: T1: Control without Cinnamon; T2: 4 litres of water + 0.1g of cinnamon; T3: 4 litres of water + 0.2g of cinnamon; T4: 4 litres of water + 0.3g of cinnamon; T5: 4 litres of water + 0.4g of cinnamon. Parameters measured were egg weight, egg mass, egg breadth, egg length, albumen height, albumen weight, yolk height, yolk length, yolk colour, yolk weight, shell weight, and shell thickness. The results revealed an increase in the albumen weight, yolk weight, albumen height, and yolk height as the inclusion levels of cinnamon powder increase. In addition, the egg weight was highest in treatment 5. Taken together, cinnamon powder addition in the diet of Lohmann Brown layer birds has a positive outcome on egg quality. Keywords— Antibiotic growth promoters, Cinnamon, Egg quality, Layers, Poultry I. INTRODUCTION Poultry production has been on the increase over the years in different parts of the world. According to research, it is the most consumed meat globally, with production peaking 137 million tons in 2020 (Ali et al., 2021). Even in developing countries like Nigeria, poultry is the fastest growing subsector of animal husbandry, with eggs being the most affordable and demanded product (Heise et al., 2015). In order to keep up with demand, antibiotic growth promoters (AGP) have long been in use as the primary growth promotant and carcass modifier. However, due to increasing consumer awareness about food safety, AGPs have been banned in most countries of the world (Beg et al., 2016). The global trend against antibiotic growth promoters in animal diets has led researchers to find safer and natural alternatives. Hence, natural products like rosemary, turmeric, cumin, and cinnamon have been in the spotlight in the past decade due to their natural medicinal properties (Rao & Gan, 2014). Cinnamon belonging to the genus Cinnamomum is the most consumed spice globally, with over 250 evergreen trees and shrubs mainly found in Asia and Australia. It has been in use in different countries for thousands of years with reference in ancient scriptures (Barceloux, 2009). As yet, research have been carried out to evaluate the effects of Cinnamon on broiler meat and quail eggs (Ciftci et al., 2010; Santos et al., 2019). This paper aimed to explore the effects of Cinnamon powder on egg quality parameters of commercial laying birds fed varying levels of cinnamon powder inclusion in their diets. II. MATERIALS AND METHODS The experiment was carried out using battery cages at the Poultry Unit of the Teaching and Research Farm at Ladoke Akintola University of Technology Ogbomoso, Oyo state. One hundred and fifty (150) point of lay birds (Lohmann Brown) were used for this experiment, and the birds were
  • 2. Usman Sulaiman et al. International Journal of Forest, Animal and Fisheries Research (IJFAF) 5(6)-2021 Int. J. Forest Animal Fish. Res. www.aipublications.com/ijfaf Page | 5 purchased at 18 weeks old from Global West Farms at Ewura in Oyo State. Before starting the experiment, the birds were allowed to acclimatize for three weeks and randomly divided into five treatments of 30 birds per treatment comprising fifteen replicates of 2 birds per replicates. They were offered adequate feed, clean and fresh water daily throughout the experiment and were submitted to a light regimen of 15 hours daily. Diet was fed to each treatment group at 7.00 am and 12.00 pm daily. The experiment started when the birds have started laying fully at 21 weeks old and lasted till 33 weeks old translating to twelve (12) weeks of data collection. The Cinnamon powder (Cinnamomum zeylanicum) used was procured from Gel Spice Company in its finished fine powder form. Layer mash and Cinnamomum zeylanicum was introduced to the commercial layers when they started laying at an inclusion level of 4litres of water in 0g, 0.1g, 0.2g, 0.3g and 0.4g of cinnamon powder. Cinnamon powder was adequately mixed to dissolve in drinking water fully to avoid settling of the powder. Five experimental diets (Cinnamomum zeylanicum + 4 litres of water) was formulated: Diet 1: Control without Cinnamon, Diet 2: 4 litres of water + 0.1g of cinnamon, Diet 3: 4 litres of water + 0.2g of cinnamon, Diet 4: 4 litres of water + 0.3g of cinnamon, and Diet 5: 4 litres of water + 0.4g of cinnamon. Eggs were collected and weighed from each replicate daily. At the end of each week, one egg was randomly selected from each replicate. It was subjected to the following measurements: Egg weight (g), egg mass (g), egg breadth (mm), egg length (mm), albumen height (cm), albumen weight (g), yolk height (cm), yolk length (mm), yolk colour, yolk weight (g), shell weight (g), shell thickness (mm). Weight measurements were carried out using an electric weighing balance. Length and breadth measurements using a vernier calliper. Height was measured using a ruler while the thickness with a micrometre screw gauge. Yolk colours were measured by comparing with an egg yolk colour fan. Data obtained from this study were subjected to analysis of variance using SAS. Significantly different means were separated using Duncan’s Multiple Range Test (DMRT) of the same package. *Premix composition per kg diet: Vitamin A 10,000,000iu, Vitamin B 13750 Mg, Vitamin B2 5000Mg, Vitamin B6 3750Mg, Vitamin B12 2000 Mg, Vitamin D3 2,500,000iu, Vitamin E30,000iu, Vitamin k3 5000Mg, Antioxidant 1250 Mg, Biotin 1500 Mg, Choline chloride 500,000Mg, Copper 6500 Mg, Cobalt 200 Mg, Folic 125 Mg, Iron 70,200 Mg, Iodine 400 Mg, manganese 75,000 Mg, Niacin (PP) 30,000Mg, Selenium 80 Mg, Zinc 85,500Mg. Table 1: Gross composition and nutrient content of experimental diets Feed Ingredients Quantity (%) Maize 35.00 Groundnut Cake 12.000 Soybean Meal 9.000 Palm Kernel Cake 15.000 Corn Bran 15.00 Fish Meal 1.200 Oyster Shell 8.000 Bone Meal 4.000 Lysine 0.150 Methionine 0.200 Premix* 0.250 Salt 0.200 TOTAL 100.000 Crude Protein (g kg-1 ) 17.890 ME (Kcal kg-1 ) 2497.270 Crude Fiber (%) 5.347 Calcium (g kg-1 ) 29.22 Available Phosphorus (g kg-1 ) 3.04 Sodium (g kg-1 ) 1.46 Chlorine (g kg-1 ) 2.42 Potassium (g kg-1 ) 7.25
  • 3. Usman Sulaiman et al. International Journal of Forest, Animal and Fisheries Research (IJFAF) 5(6)-2021 Int. J. Forest Animal Fish. Res. www.aipublications.com/ijfaf Page | 6 III. RESULTS The results of the supplementation of cinnamon powder on external egg quality characteristics of layers is shown in table 2. There were significant (p<0.05) differences among the parameters measured across the treatment except for egg length, similar all through. The highest value of the egg weight was recorded in T5 (61.32g), with the lowest value in T2 (58.24g). Egg mass was highest in T5 (54.08g), similar to T3 (53.47g), while the lowest value of 51.31g was observed in T2, similar to T1 with the value 51.87g. The birds on T5 have the highest egg breath value, 43.63g, while T1, T3 and T4 were observed to be similar with the values of T1 (43.30g), T3 (43.57g) and T4 (43.40g) with the lowest value (43.04g) recorded in T2. Table 2: Mean values of cinnamon powder on external egg quality characteristics of layers chicken Parameters T1 T2 T3 T4 T5 ±SEM Shell weight 4.63ab 4.48ab 4.39b 4.78a 4.48ab 0.47 Shell thickness 0.33b 0.32b 0.50a 0.34b 0.33b 0.13 Egg weight 59.22bc 58.24c 60.34ab 59.65abc 61.32a 2.43 Egg mass 51.87b 51.31b 53.47a 52.62ab 54.08a 2.24 Egg length 55.16a 55.43a 54.82a 55.64a 55.50a 1.67 Egg breadth 43.30ab 43.04b 43.57ab 43.40ab 43.63a 0.81 T1: Control without Cinnamon; T2: 4 litres of water + 0.1g of cinnamon; T3: 4 litres of water + 0.2g of cinnamon; T4: 4 litres of water + 0.3g of cinnamon; T5: 4 litres of water + 0.4g of cinnamon. Means along the same row with different superscripts are significantly (p<0.05) different, SEM=Standard Error Mean. Table 3 shows the mean values of cinnamon powder on internal egg quality characteristics of layers. There were significant (p<0.05) differences among the parameters measured across the dietary treatments. The value of the albumin weight was observed to be highest in T5 (38.95g), the lowest value was in T2 (36.64g). Yolk height was highest in T5 (2.24g), while the lowest was observed in chickens fed on diet T2 (2.07g). The result revealed an increase in the albumen weight, yolk weight, albumen height, and yolk height as the inclusion levels of cinnamon powder increase. Table 3: Mean values of cinnamon on internal egg quality characteristics of layers T1: Control without Cinnamon; T2: 4 litres of water + 0.1g of cinnamon; T3: 4 litres of water + 0.2g of cinnamon; T4: 4 litres of water + 0.3g of cinnamon; T5: 4 litres of water + 0.4g of cinnamon. Means along the same row with different superscripts are significantly (p<0.05) different, SEM=Standard Error Mean. Parameters T1 T2 T3 T4 T5 ±SEM Albumen weight 36.80b 36.64b 37.80ab 37.61ab 38.95a 2.14 Yolk weight 14.38a 13.93b 14.39a 13.93b 14.53a 0.15 Albumen height 1.62a 1.61a 1.63a 1.78a 1.67a 0.27 Yolk length 37.05a 36.90a 37.20a 37.04a 37.01a 1.42 Yolk height 2.11b 2.07b 2.10b 2.11b 2.24a 0.12 Yolk colour 7.45a 6.85b 7.20a 6.92a 6.63a 1.38
  • 4. Usman Sulaiman et al. International Journal of Forest, Animal and Fisheries Research (IJFAF) 5(6)-2021 Int. J. Forest Animal Fish. Res. www.aipublications.com/ijfaf Page | 7 IV. DISCUSSION In the present article, we investigated whether cinnamon affects the egg qualities of laying commercial birds. Our findings converge with Suwarta & Suryani (2019) research on quail that cinnamon powder significantly increased egg weight and egg shell weight. In addition, our study revealed for the first time that shell weight, shell thickness, egg weight, egg mass and egg breadth were significantly influenced by cinnamon powder supplementation in the diet of layer birds. Vali et al. (2013) reported that supplementation of Cinnamon zeylanicum bloom and Thymus vulgaris on the egg quality of Japanese quail significantly improved egg quality parameters, supporting the present result on egg quality and cinnamon powder. Ulku et al. (2015) on the effect of cinnamon and rosemary oils on egg quality in laying quails indicated that cinnamon oil added a positive effect on egg shell quality. Our finding revealed a significant interaction between cinnamon powder and egg weight, in line with Torki et al. (2015) and Hesham et al. (2019), who studied the effect of cinnamon essential oil on the egg quality of laying hens. Abo Ghanima et al. (2020) reported on the effect of housing system, rosemary and cinnamon essential oils on egg quality traits, reviewed that supplementation of rosemary and cinnamon essential oils in laying hens had a significant effect on egg quality characteristics. The study of Vali & Mottaghi (2016) reported the effect of using different levels of cinnamon and thyme powder on egg quality characteristics in Japanese quails reviewed that egg shell and egg shell thickness increases significantly compared to control. However, the result of this study contradicted the findings of Santos et al. (2019), who reported that the use of cinnamon in Japanese laying quail had no significant effect on performance or the egg quality variable. Except for the specific gravity and colour of the yolk, which increases linearly as the inclusion of cinnamon powder increases. The findings of Soomro et al. (2018) also disagreed with the current study that the alternative use of turmeric in induced moulting on the performance of layers had non-significant differences among the groups for egg width, yolk weight, and albumin weight. V. CONCLUSION In conclusion, the result of the study indicated that the inclusion of cinnamon powder at 0.4g/4liters to the drinking water of layers has improved egg quality compared to other dietary treatments. Thus, cinnamon can be used as a natural egg booster for layer birds. Future research may extend this work by exploring other natural AGP alternatives on different breeds of laying birds. CONFLICT OF INTEREST The authors declare that there is no conflict of interest. ETHICAL APPROVAL The two authors have declared that, “principle of laboratory animal care” (NIH publication 8023 revised 1978) were followed as well as the University law. AUTHORS' CONTRIBUTIONS This work was conducted in collaboration by both authors. Both authors have read and approved the final version of the manuscript. REFERENCES [1] Abo Ghanima, M. M., Elsadek, M. F., Taha, A. E., Abd El- Hack, M. E., Alagawany, M., Ahmed, B. M., Elshafie, M. M., & El-Sabrout, K. (2020). Effect of housing system and rosemary and cinnamon essential oils on layers performance, egg quality, haematological traits, blood chemistry, immunity, and antioxidant. Animals, 10(2), 245. https://doi.org/10.3390/ani10020245 [2] Ali, A., Ponnampalam, E. N., Pushpakumara, G., Cottrell, J. J., Suleria, H. A. R., & Dunshea, F. R. (2021). Cinnamon: A natural feed additive for poultry health and production—A review. Animals, 11(7), 1–16. https://doi.org/10.3390/ani11072026 [3] Barceloux, D. G. (2009). Cinnamon (Cinnamomum Species). Disease-a-Month, 55(6), 327–335. https://doi.org/10.1016/j.disamonth.2009.03.003 [4] Beg, M. A. H., Hossain, M. M., & Alam, M. J. (2016). Use of Cinnamon and Black Cumin as Effective Alternative of Antibiotics on Growth Performance of Broiler. International Journal of Animal Resources, 1(2), 17–26. [5] Ciftci, M., Simsek, U. G., Yuce, A., Yilmaz, O., & Dalkilic, B. (2010). Effects of dietary antibiotic and cinnamon oil supplementation on antioxidant enzyme activities, cholesterol levels and fatty acid compositions of serum and meat in broiler chickens. Acta Veterinaria Brno, 79(1), 33– 40. https://doi.org/10.2754/avb201079010033 [6] Heise, H., Crisan, A., & Theuvsen, L. (2015). The poultry market in Nigeria: Market structures and potential for investment in the market. International Food and Agribusiness Management Review, 18(SpecialIssueA), 197–222. 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  • 5. Usman Sulaiman et al. International Journal of Forest, Animal and Fisheries Research (IJFAF) 5(6)-2021 Int. J. Forest Animal Fish. Res. www.aipublications.com/ijfaf Page | 8 M., Santana, C. C. S., & Souza, D. M. (2019). The use of cinnamon powder in the diet of Japanese laying quail. Acta Scientiarum - Animal Sciences, 41(1), 1–7. https://doi.org/10.4025/actascianimsci.v41i1.42963 [10] Soomro, H., Rajput, N., Soomro, N. M., & Kalhoro, N. H. (2018). Alternative Use of Turmeric in Induce Molting on Performance of Layers. International Journal of Scientific & Engineering Research, 9, 1150–1179. [11] Suwarta, F. X., & Suryani, C. L. (2019). The effects of supplementation of cinnamon and turmeric powder mixture in ration of quail on performance and quality of eggs. Journal of World’s Poultry Research, 9(4), 249–254. https://doi.org/10.36380/SCIL.2019.WVJ31 [12] Torki, M., Akbari, M., & Kaviani, K. (2015). Single and combined effects of zinc and cinnamon essential oil in diet on productive performance, egg quality traits, and blood parameters of laying hens reared under cold stress condition. International Journal of Biometeorology, 59(9), 1169– 1177. https://doi.org/10.1007/s00484-014-0928-z [13] Ülkü Gülcihan; ÇİFTÇİ, Ş. (2015). Effects of cinnamon and rosemary oils on egg production, egg quality, hatchability traits and blood serum mineral contents in laying quails (Coturnix coturnix Japonica). Ankara Üniversitesi Veteriner Fakültesi Dergisi, 62(3), 229–236. https://doi.org/10.1501/vetfak_0000002685 [14] Vali, N., & Mottaghi, S. (2016). The effect of using different levels of cinnamon and thyme powder on egg characteristics and fatty acids profile in japanese quail. CIBTech Journal of Zoology, 5(3), 2319–3883. [15] Vali, N., Shahin, H., & Vatankhah, M. (2013). RESEARCH OPINIONS IN ANIMAL & VETERINARY SCIENCES Determination of the effects of Cinnamomum zeylanicum Blume and Thymus. Research Opinions in Animal and Veterinary Sciences, 3(9), 280–284.