Stem Cells: The Game Changer in Skin Repair and Regeneration | Dr David Greene Arizona

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Dr. David Greene Arizona as he explores groundbreaking developments in stem cell therapy, a transformative approach to skin repair and regeneration. Discover how these potent cells are revolutionizing dermatological treatments, offering hope and effective solutions for various skin conditions and aesthetic concerns.

Healthcare

Stem Cells: The Game
Changer in Skin Repair
and Regeneration

Epidermal Stem Cells
Adult stem cells reside in specific
microenvironments called niches
that are important for modulating
stem cell fate and activity. Three
distinct epidermal stem cell
niches have been identified in the
skin.

Epidermal Cell
Clonal Conversion
Over the past decade, significant
progress has been made in identifying
specific markers for stem
cell/progenitor cell isolation and
enrichment. This has been made
possible through use of mouse models,
techniques such as transplantation,
lineage-tracing, label retaining, and
fate-mapping studies, and functional
assays like in vitro colony formation.

Epidermal Stem Cells
Engage in Tissue Repair
Following Injury
In response to injury, stem cells
from the hair follicle and IFE
contribute towards re-
epithelialization of wounds. In full-
thickness wounds, cells from hair
follicles and IFE have been shown
to migrate to the wound site.

Other Hair Follicle
Stem Cells
In humans, other subpopulations
of stem cells reside within the hair
follicle such as melanocyte stem
cells, mesenchymal-like stem
cells derived from the dermal
sheath (DS) and dermal papilla
(DP), and nestin-positive stem
cells.

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Tissue regeneration is an emerging and exciting biomedical field. As a scientist, choose either an adult stem cell population or embryonic stem cells as a model system to develop a technique for growing artificial limbs in culture. Be sure to explain why you chose your particular stem cell and the drawbacks associated with the one that you did not choose for your experiments. Solution In tissue regeneration scientists choose only embryonic stem cells as a models system to develop a technique for growing artificial limbs in culture. In case of salamanders have unusual and very desirable characteristics of being able replicate limb. When salamanders loses a limb does not form scab. Instead a wound epidermis forms it known as apical epithelial cells they form blastema. This knob of stem cells is then able completely regenerate the limb, these blastemata like MRL mice act as embryonic stem cells. Embryonic stem cells: 1. It is pluripotent i.e., differentiate into 3 of the primary germ layers (ectoderm, endoderm, mesoderm) this indicates that embryonic stem cells can potentially differentiate into adult human cell if stimulated right way Drawbacks of adult stem cells: 1.It is multipotent meaning that each can only produced limited cell type. 2. Unlike embryonic stem cells, they are much scarcer and harder to culture so its not choosen by scientist to develop for growing artificial limbs in culture..

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The skin is the largest organ of the body, accounting for about 15% of the total adult body weight. It performs many vital functions, including protection against external physical, chemical, and biologic assailants, as well as prevention of excess water loss from the body and a role in thermoregulation. The skin is continuous, with the mucous membranes lining the body’s surface (Kanitakis, 2002). The integumentary system is formed by the skin and its derivative structures (see Figure 1-1). The skin is composed of three layers: the epidermis, the dermis, and subcutaneous tissue (Kanitakis, 2002). The outermost level, the epidermis, consists of a specific constellation of cells known as keratinocytes, which function to synthesize keratin, a long, threadlike protein with a protective role. The middle layer, the dermis, is fundamentally made up of the fibrillar structural protein known as collagen. The dermis lies on the subcutaneous tissue, or panniculus, which contains small lobes of fat cells known as lipocytes. The thickness of these layers varies considerably, depending on the geographic location on the anatomy of the body. The eyelid, for example, has the thinnest layer of the epidermis, measuring less than 0.1 mm, whereas the palms and soles of the feet have the thickest epidermal layer, measuring approximately 1.5 mm. The dermis is thickest on the back, where it is 30–40 times as thick as the overlying epidermis

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