Biostimulation with diode laser

1. BIOSTIMULATION WITH DIODE
LASER POSITIVELY REGULATES
CEMENTOBLAST FUNCTIONS, IN
VITRO
BOZKURT ET AL.
LASERS MED SCI 2017

2. INTRODUCTION
◦DIODE Laser
◦Low level laser light
◦Affect cell mitochondria
◦ ATP
◦Diminished inflammation modulating Reactive oxygen metabolites and cytokines
◦Improved wound healing

3. Periodontal repair/regeneration
Requires the bioactivity of periodontal cells within the periodontium
◦ cementoblasts, periodontal ligament cells, and osteoblasts
Diode lasers are not used for application on dental hard tissues, including enamel,
dentin, cementum, and bone, but the cells/stem cells of these tissues are affected
by laser light.

4. AIM OF THE STUDY
To assess whether biostimulation with a diode laser regulates cementoblasts
functions, such as proliferation and biomineralization.

5. MATERIAL AND METHODS
Sample Size
40 Healthy 3rd
molars
Root plate
1. Laser group
2. Control group
Day 2 and 5 :
Cell survival and proliferation------------ WST assay

6. DAY 3
1. SEM
3. Total RNA isolation
using
spectrophotometer.
4. Mineralization
assay
2. Laser application (Ezlase,
Biolase, USA) (InGaAsP, Indium
Gallium Arsenide Phosphate,
940 nm).
Settings:
Power: 0.3 W in continuous
wave
Irradiation time: 60 s/cm2
Energy: 18 J/cm2

7. Statistical Analysis
One-way analysis of variance (ANOVA) and
Tukey HSD multiple comparison tests

8. RESULTS:

12. Discussion
In this study, cementoblast proliferation was not changed at the beginning, but at a
later phase, cells survived longer than the untreated control group.
Considering cementoblasts remain longer within the periodontal area, this may lead
to a better attachment gain.
PDL fibroblasts, cementoblasts, and osteoblasts play a critical role in reconstructing
new attachments.

13. An animal study revealed that low-level laser irradiation improved bone healing in
tibial defects of rats.
Tim CR et al,2014
Another animal study related to orthodontics showed that low-level laser
irradiation during orthodontic force load increased both the reparative and
resorptive processes, but laser therapy has significant reparative effects on
orthodontically induced inflammatory root resorption in rats.
Altan AB et al, 2015
For von Kossa staining experiment for mineralization, we observed more
mineralized nodules in the laser-treated groups.

14. Fujji et al. Scanning Electron Microscopic Study of the Effects of Er:YAG Laser on
Root Cementum and found that the specimens showed a 15 pm layer of damaged
tissue within the laser-irradiated cementum. The tissue presented an amorphous
appearance and the Sharpey's and matrix fiber bundles were not clearly
distinguishable. These observations indicate that cementum tissue could be
damaged by Er:YAG laser irradiation.

15. Hatit et al. The Effects of a Pulsed Nd:YAG Laser on Subgingival Bacterial Flora
and on Cementum: An in Vivo Study. SEM examination of the specimens treated
with Nd:YAG laser at different levels exhibited different features of root surface
alterations.
Kriesler et al. Effect of low‐level GaAlAs laser irradiation on the proliferation rate
of human periodontal ligament fibroblasts: an in vitro study. The irradiated cells
revealed a considerably higher proliferation activity than the controls. The
differences were significant up to 72 h after irradiation.

16. LIMITATIONS
Variations in the laser used for this purpose
Set-up of lasers, and
Methodology of the evaluation of cell function.
Cells may not receive homogenous stable energy due to experimental
conditions

17. CONCLUSION
Biostimulation of cementoblasts might positively affect attachment gain since
cementoblasts play an important role in establishing the new periodontal
attachment apparatus after regenerative periodontal therapies.
Further studies are required to determine the effects of low-level laser irradiation
on different cell types and to generate established treatment protocols in
periodontology/peri-implantology.