Nexera Introduction presentation Apr19.ppt

Background of Development
How maximize sample throughput ?
 Rethinking of efficiency and productivity
 Consideration of efficiency/cost
Many UHPLCs have been commercialized during this 6 years.
 Ultra-high pressure delivery and smaller column particles are the
key for speed-up of analysis.
 All focus on pressure.
The performance is compromised in exchange for pressure ?
Flow rate limitation
Slow injection speed
Poor reproducibility
Poor durability
Limited expandability
Etc…..
?

Development Concept of Nexera
Expandability
Performance
UHPLC
UFLC
HPLC
Maximizing Throughput
Maximizing Expandability
Maximizing Performance
•Highest pressure range with
widest flow rate range.
•Fastest cycle time for maximization of sample
throughput
•Solvent consumption reduction for environment
To produce next generation UHPLC without compromise of performance
•Highest accuracy and precision in ultra fast analysis
•Fastest injection with minimized carryover
•Versatile system configuration
•Pretreatment, multi-dimensional LC with valve
control, etc.

CTO-30A
• Minimized temperature
distribution in the column
-Usable up to 150℃
LC-30AD
• World’s highest pressure
Specification 130MPa
• Ultra Low volume high
efficiency Mixer（20μL）
SIL-30AC
• 130MPa Max pressure
• 4 liquid rinsing
• AutoPretreatment
• Loop injection （optional）
Rack Changer II
• High sample capacity (4600
samples )
• front window to support
easier maintenance.
Nexera New Component Detectors • Shimadzu high-sensitivity
detector lineup, UV, PDA, RF, MS
spec…..
• High speed sampling

Nexera Features,
Ultra-high Speed/Ultra-high Resolution Analysis
•World’s highst pressure 130MPa
•Minimized system delay volume
Ultra-Fast Injection
•World’s fastest autosampler 10sec/ injection
•Minimized injection time by overlap injection
Maximized Sample Capacity
•Nexera Rack changer  4600 samples
•Multiplexed system

S4-Plunger surface(x50)
World’s Highest Pressure 130MPa
S4–Plunger （Super Smooth Surface Sapphire）
Minimized frictional heat on plunger surface
Optimized material for UHPLC
Plunger, Plunger seal, Needle seal, High-pressure valve, etc.
New high pressure valve design
High-strength
seal material
S4-Plunger
High-pressure valve

Vendor W
Vendor A
Vendor T
Vendor D
Vendor A’
MPa
bar
1200
1000
800
600
400
200
mL/min
1400
Shimadzu Nexera
Power Range

Nexera
Ultra-High
Speed
High Speed
and
High
Resolution
Ultra-High
Resolution
Enhanced Balance between Speed and Resolution
Nexera meets customers’ diverse needs in speed/resolution thanks
to the widest pressure range .

Ultra-high Speed Analysis
0.0 2.5 5.0 7.5 10.0 12.5 15.0 min
0
25
50
75
100
125
mAU
Shim-pack VP-ODS
(4.6mmi.d.150mm, 5um)
1.0 mL/min
Water/acetonitrile = 45/55
40℃
245nm
5 um
1.0 mL /min
5.4 MPa
TPN at peak 5 = 11352
Rs (4,5 = 10.849)
Zorbax eclipese plus C18 ODS
(2.1mmi.d. 50mm, 1.8 um)
0.6 mL/min
Water/acn = 45/55
40C
245nm
1. Acetophenone
2. Propiophenone
3. Butyrophenone
4. Valerophenone
5. Hexanophenone
25 times faster
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 min
1050
1075
1100
1125
1150
1175
mAU
45.0
46.0
47.0
48.0
49.0
50.0
51.0
52.0
MPa
1.8 um, 50mm
1.8mL /min
105 MPa
Rs (4,5 = 8.40)

Ultra-High Resolution
0.0 1.0 2.0 3.0 4.0 5.0 min
0
25
50
75
100
125
mAU
Shim-pack VP-ODS
(4.6mmi.d. 150mm, 5 um)
1.0 mL/min
Water/acn = 30/70
40C
245nm
1.8 um, 300 mmL
115 MPa
* 130 MPa allows to use longer columns with sub-2 um particles.
In this example, two 150mm columns with sub 1.8 um were
connected in series.
0.0 2.5 5.0 7.5 10.0 12.5 min
1
2
3
4
5
6
7
5 um, 150 mmL
4.7 MPa
(2.1mmi.d. 300mm, 1.8 um)
0.5 mL/min
Water/acn = 20/80
50C
245nm
5 times higher separation efficiency
1. Acetophenone
2. Propiophenone
3. Butyrophenone
4. Valerophenone
5. Hexanophenone
6. Heptanophenone
7. Octanophenone

Speed and Resolution
0.0 2.5 5.0 7.5 10.0 12.5 15.0 min
0
25
50
75
100
125
mAU
Shim-pack VP-ODS
(4.6mmi.d.150mm, 5um)
1.0 mL/min
Water/acetonitrile = 45/55
40℃
245nm
5 um, 150mm
1.0 mL /min
5.4 MPa
Rs (4,5 = 10.849)
(2.1mmi.d. 50mm, 1.8 um)
0.6mL/min
Water/acn = 45/55
40C
245nm
0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 min
0
25
50
75
100
125
150
175
mAU
14 times faster
1.5 times higher separation efficiency
1.8 um, 100mm
1.4 mL /min
100 MPa
Rs (4,5 = 12.21)
1. Acetophenone
2. Propiophenone
3. Butyrophenone
4. Valerophenone
5. Hexanophenone

Area
%RSD(n=6)
Peak1 0.29
peak2 0.05
peak3 0.15
Peak4 0.15
Peak5 0.26
0.0 0.1 0.2 0.3 0.4 0.5m in
0.0
1.0
2.0
3.0
4.0
5.0
6.0
m V(x100)
25.0
50.0
75.0
%
B.Conc.(Method)
Detector A:254nm
t0
1
2
3
4
5
Maximized Peak Capacity
Peak capacity =
W
tg
+1
tg : gradient time
W : peak width at 50 % of peak height
Peak capacity at peak 4 : 190 in 24 sec
Peak width at t0 : 80 msec
Reproducibility
Phenomenex Kinetex 2.1mm 30mm (2.6 um)
Column Temp : 70C
Flow : 3.0 mL/min
A: water, B : acetornitrile
GE : 2%(0min) 98% (0.40 min)
UV 254nm
t0 :Uracil
Peak 1 :Methylparaben
Peak 2 :acetophenone
Peak 3: Propyl paraben
Peak 4 :Butyl paraben
Peak 5 : Naphthalene

Peak capacity : 244 in 8 minutes
Ultra High Resolution Application
For improvement of separation efficiency in proteomic, food, pharama and
various application fields
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 min
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
mAU
BSA triptic digest
Column : Zorbax Eclipse RRHD
C18
2.1mm, 100mm, 1.8um
Mobile phase : A : 0.03 % TFA in water
B : 0.03 % TFA in acetonitrile
Gradient : B 5% → 40 % (8 min)
Mixer: 180 uL
Flow rate : 0.9 mL/min
Column temp.: 40C
Detection : 214 nm
Sample : Trypsin digested BSA
(total of 1 pmol / uL)

Minimized Delay Volume
MiRC Mixer incorporating micro reactor
technology (20 uL)
Loop injection (delay volume: 18 uL)
Low volume injection valve and injection port
High-pressure valve
MiRC mixer
Injection port

System Delay Volumes
Nexera Nexera
with 5uL fixed loop
injection
ProminenceXR
Inlet tube OD1.6xID0.3
x400mm
28.27 OD0.8xID0.1x
600mm
4.71 OD1.6xID0.3
x400mm
28.27
Auto-sampler Sample loop
(50uL)
84.82 Sample loop
(5uL)
5.00 Sample loop
(50uL)
84.82
Others 14.84 Others 1.57 Others 14.92
Outlet tube OD0.8xID0.1
x800mm
6.28 OD0.8xID0.1
X800mm
6.28 OD0.8xID0.1
x600mm
4.71
Total 134.21 17.56 132.72
Unit: mL
*Plus the selected mixer volume

Incorporating micro reactor technology (MiRC)
Split / merge flow mechanism
Multi layer structure
Lowest delay volume
standard : 20 mL
TFA applications : 180 mL
17
A
A+B
A
A
B
B
MiRC Gradient Mixer

①
④
①
②
③
④
⑤
⑥
⑦
⑥
⑤
③
②
Loop
injection
A
B
C
D
E
①
④
①
②
③
④
⑤
⑥
⑦
⑥
⑤
③
②
Direct
injection
B
C
D
E
Tube B LPV① - HPV②
Tube C Pump - HPV⑥
Tube D HPV⑤ - column
Tube E HPV③ - drain valve
Tube A Sample loop (1.5uL/5uL/20uL)
Tube B LPV① - sample loop
Tube C Pump – HPV②
Tube D HPV① - column
Tube E HPV⑤ - drain valve
Flow Diagram for Fixed Loop Injection
HPV
LPV
Drain
valve
Column
Pump
HPV
LPV
Drain
valve
Column
Pump

Column :Kinetex 2.1mm, 50mm, 2.6um
Mobile phase :A: water :B: acetonitrile
Gradient :0min (B.15%), 1min (B 80%), 1.01min(B15%),
Flow rate :0.8mL/min
Methylparaben:50ppm, Acetophenone :50ppm
Propylparaben:50ppm, Butylparaben :50ppm
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1
min
-25000
0
25000
50000
75000
100000
125000
150000
175000
200000
225000
250000
275000
300000
325000
uV
Direct inj
Fixed loop inj
Minimized Gradient Delay
Minimized internal volume in mobile phase path
254nm

Column :Kinetix 2.1mm, 50mm, 2.6um
Mobile phase :A: water :B: acetonitrile, A/B =7/3
Flow rate :0.8mL/min
Methylparaben:50ppm、Acetophenone:50ppm
Propylparaben :50ppm、Butylparaben:50ppm
When isocratic conditions are used, width and
theoretical plates # of early eluted peaks are
improved.
Rt k'
Peak width50% (sec) Theoretical plates
Direct inj Loop inj Direct inj Loop inj
uracil 0.15min - 0.610 0.495 - -
methy paraben 0.42min 1.8 0.777 0.649 5073 7006
acetophenone 0.61min 3 0.966 0.865 7346 8880
propylparaben 1.32min 7.8 2.033 1.926 8173 9036
butylparaben 2.75min 17.2 4.237 4.150 8808 9232
0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 min
0
25000
50000
75000
100000
125000
150000
uV
0.30 0.35 0.40 0.45 min
0
25000
50000
75000
100000
125000
150000
175000
uV
Total inj
Loop inj
Loop inj
Total inj
Methl Paraben
254nm
Improved Peak Width

World Fastest Autosampler
Reliable X-Y-Z needle carrying mechanism to enable high
speed motion
Ultra-low carryover design eliminates extra rinse time.

Overlap Injections
Injection Analysis Injection Analysis Injection Analysis
Start
0 min 0 sec
Injection Analysis Injection Analysis Injection Analysis
Other UHPLC
30sec for injection
Simadzu UFLC
10sec for injection
Injection Analysis
Injection Analysis
Injection Analysis
Finish
3 min 0 sec
Finish
2 min 0 sec
Finish
1 min 40 sec
33% Faster
44% Faster
Nexera with
overlapping
injection

24
Example of Overlap Injections
30 % cycle time reduction (in case of 0.7 min/1 cycle)
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 min
0
10
20
30
40
50
60
70
80
90
mV
0
50
100
150
200
250
300
350
400
450
kgf/cm2
ŒŸ
oŠíA 245nm
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 min
0
10
20
30
40
50
60
70
80
90
mV
0
50
100
150
200
250
300
350
400
450
kgf/cm2
ŒŸ
oŠíA 245nm

25
Mobile phase
Flow amount (L)
100MPa 130MPa
Water 176.4 25.2
100 mM phosphate
buffer
80.6 20.2
Methanol 156.2 22.0
Acetonitrile 141.1 25.2
Longer seal life supports UHPLC analysis.
At even 100 MPa, seal life of about half year

Nexera Features,
Ultra-high Speed/Ultra-high Resolution Analysis
•World’s highst pressure 130MPa
•Minimized system delay volume
Ultra-Fast Injection
•World’s fastest autosampler 10sec/ injection
•Minimized injection time by Overlap injection
Maximized Sample Capacity
•Nexera Rack changer  4600 samples
•Multiplexed system

Rack Changer for More Sample Capacity
Provides over 4600 consecutive sample analysis
96 well, 384 well, and vial plates
coolable rack changer (4 to 40 ºC)

Multiplexed System for Higher Throughput
The multiplexed system (MPX) is a dual stream multiplexing solution to
increase LCMS/MS throughput. The samples are alternately injected from the
two UPLC lines without dead time.

Nexera Features,
Minimized carry over for trace analysis
•Flow-through needle with minimized contact area
•Thorough rinse of sample path and needle surface by multiple
solvents (up to 4)
Precise and accurate injection at lower volume
•Direct injection with high-resolution metering pump
•Metering pump isolated from flow path
Highly-reproducible gradient at ultra high speed conditions
•High-resolution gradient control
•Efficient mixing mechanism by MiRC mixer
Uniform column heating for UHPLC
•Minimized temperature distribution across column by IHB
•Efficient preheating by small volume preheater

Nexera Features,
solvents (up to 4)
•Minimized temperature distriution across column by IHB

Minimized Carryover
Flow-through needle design
to prevent carryover
Thorough rinse of sample path
by multiple rinse
Dip wash and active wash of needle surface

Carryover of neutral compounds (Caffeine ) @ 100 MPa
Specification : < 0.0015%
Results : 0.0004 %
Without injection port rinse, needle internal rinse and needle outer
surface rinse
Carryover
Caffeine 2000 ng/mL －
Post-blank 1 0.0004%
Post-blank 2 N.D.
Column : ODS (2.0 mmI.D.×100 mmL., 1.8 um)
Mobile Phase: : Methanol, Water= 2/8
Temperature : 40 ℃
Injection volume : 5 mL
Carryover - Caffeine -
Caffeine (4 g/L)
0 1.0 2.0 3.0 4.0 5.0 min
0.0
1.0
2.0
3.0
4.0
5.0
mAU
Blank
0.0004%

Carryover
Chlorhexidine 2,000 ng/uL －
Carryover of chlorohexidine @ 100 MPa
Specification : < 0.0015%
Results : 0.0006 %
Without injection port rinse, needle internal rinse
With needle outer surface rinse
Carryover
Rinse solution : 0.05 % formic acid in methanol
Needle wash : outer surface flush by rinse pump (1 second flush)
: needle soak wash (0 second)
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min
-1.0
-0.5
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0mV
50
60
70
80
90
100
110
MPa
Detection :260nm
Carryover - Chlorohexidine -

Carryover
Chlorhexidine 2,000 ng/uL －
Post-blank 1 N.D.
Carryover
Carryover of chlorohexidine @ 100 MPa
Specification : < 0.0015%,
Results : Not detected
With injection port rinse, needle internal and outer surface rinse
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min
-1
0
1
2
3
4mV
50
60
70
80
90
100
110
MPa
Detection :260nm
Rinse solution : 0.05 % formic acid in methanol
Needle wash : outer surface flush by rinse pump (1 second flush)
: needle soak wash (0 second)
: needle internal rinse
Injection port rinse : performed
Carryover - Chlorohexidine -

Nexera Features,
solvents (up to 4)
•Minimized temperature distriuition across column by IHB

Injection volume precision & linearity in the range of 1 to 50 mL @ 100 MPa
N=6 x 5set %RSD (Area) Spec.
1 mL x 6 0.17 %
2 mLx 6 0.23 % －
5 mLx 6 0.06 % < 0.25 %
10 mLx 6 0.11 % －
20 mLx 6 0.12 % －
50 mLx 6 0.10 % －
Injection
volume
Peak Area
(n=6)
Linearity
1 mL 63857 3.4 %
2 mL 129668 1.9 %
5 mL 329103 0.4 %
10 mL 664574 - 0.5 %
20 mL 1328747 - 0.5 %
50 mL 3305754 ±0.0 %
Correlation
Coefficient
0.999996 > 0.999
Sample : Caffeine 20 mg/L
Precision Linearity
Injection Precision and Linearity in Direct mode

Nexera Features,
•Through rinse of sample path and needle surface by multiple
solvents (up to 4)
•Minimized temperature distribution across column by IHB

Time
%B
High
resolution
gradient
control
Conventional gradient control
1/6
High Resolution Gradient Control

Gradient Reproducibility-
Zorbax Eclipse plus C18, 2.1mm, 50 mm,
Water / Acetonitrile 15 → 95% (0.80min)
1.2 mL/min
20 uL mixer
40C
245nm
2 uL injection, 25ppm
R.T.
%RSD
Area R.T.
Acetophenone 0.51 min 0.156 0.068
Propiophenone 0.62 min 0.278 0.039
Butyrophenone 0.70 min 0.108 0.034
Valerophenone 0.77 min 0.259 0.045
Hexanophenone 0.84 min 0.196 0.042
Heptanophenone 0.90 min 0.260 0.034
Octanophenone 0.96 min 0.220 0.033 0.45 0.50 0.55 0.60 0.65 0.70 0.75 0.80 0.85 0.90 0.95 1.00 min
0
25000
50000
75000
100000
125000
150000
175000
200000
225000
uV
N=6 (overlay)
Excellent gradient reproducibility
even in the ultra fast analysis.

Uniformed Column Heating
IHB (Intelligent Heat Balancer )
Independently controllable two heaters to minimized temperature distribution
across column
Low volume preheater (< 3uL)

Nexera Features,
Scalable modular UHPLC design
•Wide range-configurable system by combination of conventional
HPLC units
Auto sample pretreatment by autosampler
•Precolumn derivatization
•Internal standard addition, sample dilution
High temperature analysis150℃ Column oven
•Wide temperature range control by Column oven CTO-30A (up to
150 ℃)
•Use of new technology area such as green chromatography
High speed LCMS analysis
•Combination with ultra fast LCMS, LCMS-2020

Multi Dimensional Configuration with valve control

1ppmX1μL (n=6)
1
2
3
4
5
6
7
8
9
10
11
1.Norfloxacin 2.Ofloxacin 3.Ciproploxacin
4.Danofloxacin 5.Enrofloxacin 6.Orbifloxacin
7.Sarafloxacin 8.Difloxacin 9.Oxolinic acid
10.Nalidixic acid 11.Flumequine
Use of High Sensitive RF Detector
Ultra-high sensitive RF detector, RF-20A/RF-20AX
water/raman S/N ratio : >2000 (RF-20AXS)
Stable analysis by temperature controlled cell
Quinolones analysis by Nexera-RF
Ex.295nm Em.455nm (0-1.05min)
Ex.325nm Em.365nm, (1.05-3min)

Scalable Pump Configuration
Isocratic, binary and ternary gradient are available.
MiRC mixer allows gradient elution by combination of various mobile phases.
Analysis of DNPH-aldehydes/ketones by ternary GE
0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 min
-5000
0
5000
10000
15000
20000
25000
30000
35000
40000
45000
50000
55000
60000
65000
70000
75000
80000
uV
1
2
3 4
5 6 7 8
9 10 11 12 13
1. Formaldehyde
2. Acetaldehyde
3. Acetone
4. Acrolein
5. Propionaldehyde
6. Crotonaldehyde
7. 2-Butanone
8. Methacrolein
9. N-Butylaldehyde
10. Benzaldehyde
11. Valeraldehyde
12. m-Tolualdehyde
13. Hexaldehyde
Column : Phenomenex Kinetex , 2.1mm, 50mm, 2.6 um
Mobile phase : A: water / B :acetonitrile / C : THF (Ternary gradient)
Flow rate : 0.9mL/min
Column temp. : 50C
Detection : 360 nm

0.0 0.5 1.0 1.5 2.0 2.5 min
0.0
2.5
5.0
7.5
10.0
12.5
mAU
Higher pressure capability allows alternate solvent usage for selectivity purpose.
Analysis of cortisone and cortisol in Urine (each 5 ppm, spiked; UV @ 254nm; 40C)
Mobile phase
: A :0.1 % formic acid in water
: B : acetonitrile
Mobile phase
: A : 0.1 % formic acid in water
: B : methanol
Cortisone
Cortisol
0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 min
0
5
10
15
20
25
30
mAU
Cortisol
Cortisone
Methanol
ACN : 69 MPa
Methanol : 108 MPa
Use of Alternate Solvents for Separation

49
Prelable derivatization, internal standard addition, sample dilution, etc.
0.0 1.0 2.0 3.0 4.0 5.0 min
Asp
Ser
Gly
Thr
Arg
His
Ala
Tyr
Val
Met
Ile
Phe
Leu
Lys
Glu
Arg
Val
Ile
Leu
Amino acid standard
Analysis of BCAA in
supplement
[Conditions]
Column : ODS column (3.0mm i.d. x 50mm, 2.2μm)
Mobile phase : A :20mmol/L Potassium Phosphate Buffer
: B :aceotnitrile
Column temp. : 40 ℃
Detection : Fluorescence detection
(Ex. 350nm, Em 450nm)
Auto Sample Pretreatment by SIL-30AC
Analysis of OPA derivatized amino acids

Uniform temperature control at high temperature conditions by CTO-30A oven
Optional post-column cooler minimize noise increase at high temperature conditions.
Reliable High-Temperature Control

Mobile phase: water/acetonitrile (3/7, v/v),
Detection: absorbance at 245 nm.
Peaks; 1: actophenone
2: propiophenone
3: butyrophenone
4: balenophenone
5: hexanophenone
6: heptanophenone
7: octanophenone
Improved Column Efficiency by Elevated Temperature
Column efficiency improves as column temperature increases.
Increased theoretical plate number
Decreased column pressure
4 5
6
7
0.00 0.25 0.50 0.75 1.00 1.25 min
-300
-250
-200
-150
-100
-50
0
50
100
150
mAU
0.00 0.25 0.50 0.75 1.00 1.25 min
-150
-100
-50
0
50
100
150
200
250
300
350
mAU
0.00 0.25 0.50 0.75 1.00 1.25 min
0
50
100
150
200
250
300
350
400
450
500
mAU
1
2 3
Shim-pack XR-ODS (3.0 mmi.d. ´ 50 mm , 2.2mm)
Temperature : 80°
C (Pressure : 11.8MPa)
N for Peak 7 : 9,886
Temperature : 60°
Temperature : 40°
4 5
6
7
0.00 0.25 0.50 0.75 1.00 1.25 min
-300
-250
-200
-150
-100
-50
0
50
100
150
mAU
0.00 0.25 0.50 0.75 1.00 1.25 min
-150
-100
-50
0
50
100
150
200
250
300
350
mAU
0.00 0.25 0.50 0.75 1.00 1.25 min
0
50
100
150
200
250
300
350
400
450
500
mAU
1
2 3
Shim-pack XR-ODS (3.0mm i.d., 50mmL, 2.2 um)
Temperature : 80℃ (Pressure : 11 MPa)

High temperature analysis (mobile phase : only water)
Green HPLC
1.0 2.0 3.0 4.0 5.0 6.0 min
Conventional LC
Green LC 1 : Theophylline
2 : Caffeine
1
2
1
2
150℃
Water only
40℃
30% methanol
Green LC
Column : Shodex ET-RP1 (3.0mm,150mm)
Mobile phase : water
Column temp. : 150C
Conventional LC
Column : ODS (4.6mm, 150mm, 5 um)
Mobile phase : methanol / water = 3/7
Column temp. : 40C
Green HPLC

Nexera Features,
Scalable modular UHPLC design
•Wide range-configurable system by combination of conventional
HPLC unit
Auto sample pretreatment by autosampler
•Precolumn derivatization
•Internal standard addition, sample dilution
High temperature analysis150℃ Column oven
•Wide temperature range control by Column oven CTO-30A (up to
150 ℃)
•Use of new technology area such as green chromatogaphy
High speed LCMS analysis
•Combination with ultra fast LCMS, LCMS-2020

UF scanning technology
Fastest scan speed of 15,000 u/sec
UF switching technology
High-speed polarity switching between +/- ion modes
-15 msec interval -
UF sensitivity
Superior sensitivity for ultra fast analysis
UF switching UF sensitivity UF scanning
LCMS-2020

Positive
1. Atenolol (267)
2. Procaine (237)
3. Lidocaine (237)
4. Atropine (290)
5. Yohimbine (355)
6. Chlorpheniramine (275)
7. Propranolol (260)
8. Alprenolol (250)
9. Tetracaine (265)
10. Diphenhydramine (256)
11. Doxepin (280)
12. Dipyridamol (505)
13. Desipramine (267)
14. Imipramine (281)
15. Nortriptyline (264)
16. Amitriptyline (278)
17. Dibucaine (344)
18. Verapamil (455)
19. Reserpine (609)
20. Carbamazepine (237)
21. Isopropylantipyrine (231)
22. Alprazolam (309)
23. Trizolam (343)
24. Cilostazol (370)
25. Nifedipine (347)
26. Diazepam (285)
27. Warfarin (309)
Negative
1. Cefuroxime (423)
2. Chloramphenicol (321)
3. Nitrendipine (359)
Column : Zorbax Eclipse RRHD C18 2.1mm, 50mm, 1.8um
Mobile phase : A : 0.1 % formic acid in water / B : 0.1 % formic acid in acetonitrile
Gradient : B 3% → 95 % (0.50 min)
Detection : ESI (+/-), LCMS-2020 , Scan speed : 15000 u/sec
Ultra-Fast Analysis by Nexera-LCMS-2020
Nexera-LCMS-2020
Analysis of 30 pharmaceuticals by high-speed polarity switching
(15 msec) and high-speed scanning technology (15000 u/sec)

0.0 0.1 0.2 0.3 0.4 0.5 0.6 min
0
25000
50000
75000
100000
125000
150000
175000
200000
225000
250000
275000
300000
0.0 0.1 0.2 0.3 0.4 0.5 0.6 min
0
25000
50000
75000
100000
125000
150000
175000
200000
225000
250000
275000
300000
Injection 1 Injection 300
Highly-Reproducibe High Speed Scan
LCMS-2020 offers stable high-speed scan and catches any UHPLC peaks.
The reproducible high speed scan provided very reproducible LCMS
chromatogram during 300 injections.

Minimizing Operation Cost
•Solvent Saving
・Flow Rate : 3/5 reduction x Analysis time : 1/25 reduction
= Mobile phase consumption/analysis is reduced to 1/40
•Cost for power
・Analysis Time : 1/25 reduction
= Power consumption is reduced to 1/25
•Cost for performance
・Initial cost + 5 years maintenance cost
> up to x 1.3 only
・Sample / System
> up to x 5
= Cost / performance is reduced to 1/15
•Cost saving for method migration
・Method migration （conventional LC ↔ UHPLC) tool
available

• Maximized Throughput
⇒ 25 times faster analysis cycle, 10 sec injection speed , 2300 samples/day
⇒ Solvent consumption reduced to 1/40
– Cost saving and environmentally-friendly UHPLC
• Maximized Performance
⇒ 5 times higher separation efficiency
⇒ Carryover reduced to one/third
• Maximized Expandability
⇒ Expanded configurations by more than 100 optional units
⇒ World’s highest sensitivity detectors
– PDA : 6x10-6Au, RF-20AXS : water/raman S/N>2000
Nexera will improve lab productivity by,

Nexera Introduction presentation Apr19.ppt

Nexera Introduction presentation Apr19.ppt

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Nexera Introduction presentation Apr19.ppt