Need a strong biologist/geneticist to answer this P element question for Drosophila... PLEASE What do you know about P-elements in Drosophila? How might P-elements have entered Drosophila melanogaster? What do you know about P-element structure? How big are the native elements? How do P-elements move around the genome? Connect your ideas to the special P- elements used in this lab. What is the connection between transposition of mobile DNA elements and DNA replication? Why are P-elements important for biologists and Drosophila researchers in particular? What do you know about the Gene Disruption Project (GDP) in Drosophila? Solution Ans.) P element is a type of transposon found particularly in Drosophila and is used for mutagenesis and for the production of genetically modified flies that is extensively used in various genetic and medical researches. It has ability to initiate a phenotype known as hybrid dysgenesis. It encodes the protein P transposase and is flanked by terminal inverted repeats which are important for its mobility. The P element enters in Drosophila by the horizontal gene transfer method. It has a sequence of 4 exons with 3 introns. The absolute splicing of the intron produces the transposase enzyme, whereas the alternative partial splicing of intron 1 and 2 produce the P element repressor. The P element is a class II transposon and it is moved by DNA-based \"cut and paste\" mechanism. The P element encodes the transposase enzyme which recognizes the 31 bp terminal inverted repeats of the P element and catalyzes P element excision and re-insertion. P element is important for biologist and genetics because nowadays it is widely used to solve many puzzles of genetics through its use in fly transformation, insertional mutagenesis as well as reverse genetics. The Gene Disruption Project (GDP) is a public collection of mutant that is created by applying a non-targeted transposon mutagenesis strategy. Its main objective is to acquire a transposable element insertion in each gene and this collection is available online and new mutants may be requested while they are being balanced. These balanced stocks are available at the Bloomington Drosophila Stock Center..

1. Need a strong biologist/geneticist to answer this P element question for Drosophila... PLEASE
What do you know about P-elements in Drosophila? How might P-elements have entered
Drosophila melanogaster? What do you know about P-element structure? How big are the native
elements? How do P-elements move around the genome? Connect your ideas to the special P-
elements used in this lab. What is the connection between transposition of mobile DNA elements
and DNA replication? Why are P-elements important for biologists and Drosophila researchers
in particular? What do you know about the Gene Disruption Project (GDP) in Drosophila?
Solution
Ans.) P element is a type of transposon found particularly in Drosophila and is used for
mutagenesis and for the production of genetically modified flies that is extensively used in
various genetic and medical researches. It has ability to initiate a phenotype known as hybrid
dysgenesis. It encodes the protein P transposase and is flanked by terminal inverted repeats
which are important for its mobility.
The P element enters in Drosophila by the horizontal gene transfer method. It has a sequence of 4
exons with 3 introns. The absolute splicing of the intron produces the transposase enzyme,
whereas the alternative partial splicing of intron 1 and 2 produce the P element repressor.
The P element is a class II transposon and it is moved by DNA-based "cut and paste"
mechanism. The P element encodes the transposase enzyme which recognizes the 31 bp terminal
inverted repeats of the P element and catalyzes P element excision and re-insertion.
P element is important for biologist and genetics because nowadays it is widely used to solve
many puzzles of genetics through its use in fly transformation, insertional mutagenesis as well as
reverse genetics.
The Gene Disruption Project (GDP) is a public collection of mutant that is created by applying a
non-targeted transposon mutagenesis strategy. Its main objective is to acquire a transposable
element insertion in each gene and this collection is available online and new mutants may be
requested while they are being balanced. These balanced stocks are available at the Bloomington
Drosophila Stock Center.