Where does the Calvin cycle take place? stroma of the chloroplast thylakoid membrane thylakoid space outer membrane of the chloroplast cytoplasm In a plant cell, where are ATP synthase complexes located? A. thylakoid membrane B. Inner mitochondrial membrane C. plasma membrane D. A & B E. A, B, and C Solution 9. Stroma of the chloroplasts Calvin cycle takes place inside the stroma of the chloroplasts. 10. The answer is D. Both Aand B. In plants ATP synthase complexes are present in the thylakoids membrane and in the inner mitochondrial membrane..