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Describe the basic properties of DNA that are responsible for fiber formation when alcohol is
added to a solution that contains native (double-stranded) DNA. Describe the type of precipitate
that is formed when alcohol is added to denatured (single stranded) DNA. Offer an explanation
as to why this precipitate is different from that which is observed with native DNA. Describe the
action of DNAse I on DNA and relate this effect to the results of your experiment in Section III.
Solution
1).
The highly polar phosphate backbone of the DNA and the sugar molecules makes the DNA to
dissolve in water because water is highly polar, “like dissolves like.”
In contrast to water, ethanol is less polar and the polarity decreases with increasing carbon
number. So, when ethanol and positive ions are added to the solution, the charges are disrupted
and the phosphate ions become free. The negatively charged phosphate ions combine with the
positive ions and form ionic bonds, which is visible as DNA precipitation.

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